FastQCFastQC Report
Thu 2 Feb 2017
SRR4063056_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063056_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101060
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24610.22351188854376694No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22110.20080649555882513No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19560.17764699471418452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT19390.17610302799120847No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19230.1746498828401722No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19040.1729242729733166No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18450.16756580022887035No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17790.16157157648084572No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17100.15530488801700182No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16900.15348845657820645No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15180.1378671462045665No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15150.1375946814887472No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14560.13223620874430095No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14440.13114634988102375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT14370.13051059887744537No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14190.12887581058252956No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14020.1273318438595535No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13000.11806804352169727No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT12740.11570668265126333No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT12710.11543421793544402No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC12650.11488928850380542No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12610.11452600221604635No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT12570.11416271592828728No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG12530.11379942964052824No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG12500.11352696492470891No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12430.11289121392113054No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12280.11152889034203403No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12080.1097124589032387No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT11840.1075327411766843No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG11830.10744191960474453No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC11260.10226509000417779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATGC350.0020666922.8567543
TAGACCG400.004482626420.0005685
CAAGACG4700.019.4039254
CTAGCGG4100.019.12162629
TAACCCG1450.018.758328
AAGACGG4900.018.6127725
GCGCAAG4750.018.5302091
TCTAGCG4150.018.50570928
GACGGAC4850.018.474757
CGCCCCT2350.018.3835015
AACCCGT1500.018.13302429
TACGCTA4100.017.9517299
TATTGGG450.00885091217.7774752
TAGCGGC4450.017.61767830
CGCAAGA5000.017.5996992
AGGGGCG3100.017.54808832
GTACTAA550.001362676617.4582141
ACGGACC5250.017.3719228
ATATACG4350.017.2878466
CGGACCA5200.017.2312569
Produced by FastQC (version 0.11.5)