##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063056_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1101060
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166724792472706	32.0	32.0	32.0	32.0	32.0
2	31.21068879080159	32.0	32.0	32.0	32.0	32.0
3	31.32834722903384	32.0	32.0	32.0	32.0	32.0
4	31.43926216554956	32.0	32.0	32.0	32.0	32.0
5	31.34941238442955	32.0	32.0	32.0	32.0	32.0
6	34.876530797595045	36.0	36.0	36.0	36.0	36.0
7	34.89911993896791	36.0	36.0	36.0	36.0	36.0
8	34.83175485441302	36.0	36.0	36.0	32.0	36.0
9	34.9601929050188	36.0	36.0	36.0	32.0	36.0
10	34.777642453635586	36.0	36.0	36.0	32.0	36.0
11	34.97763972898843	36.0	36.0	36.0	36.0	36.0
12	34.85959529907544	36.0	36.0	36.0	32.0	36.0
13	34.92370443027628	36.0	36.0	36.0	32.0	36.0
14	34.86062975677983	36.0	36.0	36.0	32.0	36.0
15	34.830641381941035	36.0	36.0	36.0	32.0	36.0
16	34.83482371532887	36.0	36.0	36.0	32.0	36.0
17	34.79323742575337	36.0	36.0	36.0	32.0	36.0
18	34.803898061867656	36.0	36.0	36.0	32.0	36.0
19	34.79707736181498	36.0	36.0	36.0	32.0	36.0
20	34.784128930303524	36.0	36.0	36.0	32.0	36.0
21	34.77195339036928	36.0	36.0	36.0	32.0	36.0
22	34.75898225346484	36.0	36.0	36.0	32.0	36.0
23	34.684351443154775	36.0	36.0	36.0	32.0	36.0
24	34.661893993061234	36.0	36.0	36.0	32.0	36.0
25	34.63912684140737	36.0	36.0	36.0	32.0	36.0
26	34.57367990845186	36.0	36.0	36.0	32.0	36.0
27	34.55257933264309	36.0	36.0	36.0	32.0	36.0
28	34.52458539952409	36.0	36.0	36.0	32.0	36.0
29	34.48067044484406	36.0	36.0	36.0	32.0	36.0
30	34.4552158828765	36.0	36.0	36.0	32.0	36.0
31	34.45807040488257	36.0	36.0	36.0	32.0	36.0
32	34.43263219079796	36.0	36.0	36.0	32.0	36.0
33	34.39214665867437	36.0	36.0	36.0	32.0	36.0
34	34.381280765807496	36.0	36.0	36.0	32.0	36.0
35	34.34784934517646	36.0	36.0	36.0	32.0	36.0
36	34.31074873303907	36.0	36.0	36.0	32.0	36.0
37	34.318010825931374	36.0	36.0	36.0	32.0	36.0
38	33.831096398016456	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	20.0
22	88.0
23	243.0
24	653.0
25	1752.0
26	3821.0
27	7569.0
28	13132.0
29	21320.0
30	32563.0
31	47134.0
32	68200.0
33	110636.0
34	262397.0
35	531527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15609056484495	18.35230622086058	11.854686422812154	26.636916791482317
2	16.059615279821262	20.304797195429856	36.137903474833344	27.497684049915538
3	18.000653915317965	24.01767387789948	28.52850889143189	29.45316331535066
4	11.8777683625825	15.31455683039116	36.03773464952314	36.7699401575032
5	14.283884878103716	36.818462952484836	33.09059732493899	15.807054844472457
6	35.22572359882622	35.79544783703689	15.78380450093955	13.195024063197339
7	30.749955497106402	30.203932239349278	20.384050889512938	18.66206137403138
8	28.82225681584105	32.606140750818774	18.920146261981877	19.6514561713583
9	27.24940192148051	13.579969955296242	18.22636341012837	40.944264713094874
10	15.910572960090969	25.987826210158634	31.017915560908005	27.0836852688424
11	39.00239052798698	20.68306249159864	21.18659923779214	19.127947742622244
12	24.57084419789348	23.69668418619563	27.99119391639125	23.741277699519646
13	29.521114275749827	18.564178388746804	25.296624331550806	26.61808300395257
14	23.640673532777505	19.55261293662471	24.13719506657221	32.66951846402558
15	25.570268650209798	27.00116251612083	21.68510344577044	25.743465387898933
16	26.621098415252952	25.355407839180284	22.99649700878881	25.026996736777956
17	24.475777886763662	25.837465714856595	24.583855557371987	25.10290084100776
18	25.9594390859717	23.955279457976857	25.782791128548855	24.30249032750259
19	25.944998456033275	24.841970464824804	24.736708262946618	24.476322816195303
20	26.00748369752784	23.624416471400288	24.55188636404919	25.816213467022685
21	27.543573959251955	23.91197928539706	23.594103494908083	24.9503432604429
22	25.938005194993913	24.02212413492453	24.635351388661835	25.404519281419724
23	24.21343834748033	23.49667319977694	25.392576957798774	26.897311494943953
24	24.913719506657223	24.860770530216335	24.67177083900968	25.55373912411676
25	25.16740701451966	24.083450568952255	24.772514461077925	25.97662795545016
26	24.91149421738001	25.13900266834263	25.247353000477723	24.702150113799636
27	25.943659694893732	24.256738285595958	24.346651723477127	25.452950296033183
28	24.635987139665414	24.084791019562967	25.61122917915463	25.66799266161699
29	24.53499355166839	24.538626414545984	25.46891177592502	25.457468257860604
30	24.499209852324125	24.818720142408225	25.683977258278386	24.998092746989265
31	25.294534357800664	24.605743556209468	24.175067662071097	25.92465442391877
32	25.018164314387953	24.49212576971282	24.22438377563439	26.265326140264833
33	24.467059015857444	23.97280802136123	25.112527927633373	26.447605035147948
34	25.454289502842713	24.366792000435943	25.320872613663198	24.858045883058143
35	26.455415690334767	23.894156540061395	25.099994550705684	24.550433218898153
36	24.66813797613209	25.123517337838088	24.55542840535484	25.652916280674987
37	26.235899950956348	24.686211468948105	24.479501571213195	24.598387008882348
38	24.9303398543222	24.018672915190816	25.029153724592668	26.02183350589432
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	30.5
2	36.0
3	36.0
4	155.5
5	275.0
6	275.0
7	385.5
8	496.0
9	540.0
10	584.0
11	584.0
12	770.5
13	957.0
14	1371.0
15	1785.0
16	1785.0
17	2666.5
18	3548.0
19	3548.0
20	4389.5
21	5231.0
22	6078.0
23	6925.0
24	6925.0
25	8511.0
26	10097.0
27	10097.0
28	12946.0
29	15795.0
30	19335.0
31	22875.0
32	22875.0
33	29767.5
34	36660.0
35	36660.0
36	40983.0
37	45306.0
38	52345.0
39	59384.0
40	59384.0
41	61891.0
42	64398.0
43	74711.5
44	85025.0
45	85025.0
46	87469.0
47	89913.0
48	89913.0
49	95442.5
50	100972.0
51	102330.5
52	103689.0
53	103689.0
54	98532.0
55	93375.0
56	93375.0
57	91192.5
58	89010.0
59	80451.5
60	71893.0
61	71893.0
62	69958.0
63	68023.0
64	56987.0
65	45951.0
66	45951.0
67	38718.5
68	31486.0
69	31486.0
70	25316.5
71	19147.0
72	15063.5
73	10980.0
74	10980.0
75	8108.0
76	5236.0
77	5236.0
78	5242.5
79	5249.0
80	4062.0
81	2875.0
82	2875.0
83	2703.5
84	2532.0
85	2532.0
86	1621.5
87	711.0
88	583.5
89	456.0
90	456.0
91	276.0
92	96.0
93	67.0
94	38.0
95	38.0
96	25.5
97	13.0
98	13.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009354621909796015
2	0.0
3	0.0
4	2.724647158193014E-4
5	9.082157193976714E-5
6	9.082157193976714E-5
7	7.265725755181371E-4
8	0.001998074582674877
9	0.0030879334459520823
10	5.449294316386028E-4
11	0.004359435453108822
12	2.724647158193014E-4
13	3.6328628775906857E-4
14	0.0
15	0.0
16	9.082157193976714E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	9.082157193976714E-5
22	0.0
23	1.8164314387953429E-4
24	0.0
25	9.082157193976714E-5
26	1.8164314387953429E-4
27	9.082157193976714E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1101060.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.56663911616908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.51783796948546	36.87263023760183
2	13.39752274716195	12.745502591298372
3	3.995649775638146	5.701788927371449
4	1.63469112720328	3.1102705165632827
5	0.8453446400226778	2.0105101710373283
6	0.5236407859958049	1.4944699376381745
7	0.35350249178657744	1.1770447817435055
8	0.23216407532826677	0.8834611829503007
9	0.19088687688300696	0.8171862466235931
>10	1.064372798243827	9.458945135603368
>50	0.1055359887707502	3.5679543349161214
>100	0.11529398029365906	11.87336070838761
>500	0.01647056840689952	5.445612296493111
>1k	0.0070861747797125825	4.8412629317719755
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2461	0.22351188854376694	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2211	0.20080649555882513	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1956	0.17764699471418452	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1939	0.17610302799120847	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1923	0.1746498828401722	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1904	0.1729242729733166	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1845	0.16756580022887035	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1779	0.16157157648084572	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1710	0.15530488801700182	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1690	0.15348845657820645	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1518	0.1378671462045665	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1515	0.1375946814887472	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1456	0.13223620874430095	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1444	0.13114634988102375	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1437	0.13051059887744537	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1419	0.12887581058252956	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1402	0.1273318438595535	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1300	0.11806804352169727	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1274	0.11570668265126333	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1271	0.11543421793544402	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1265	0.11488928850380542	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1261	0.11452600221604635	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1257	0.11416271592828728	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1253	0.11379942964052824	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1250	0.11352696492470891	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1243	0.11289121392113054	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1228	0.11152889034203403	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1208	0.1097124589032387	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1184	0.1075327411766843	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1183	0.10744191960474453	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1126	0.10226509000417779	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.082157193976713E-5	0.0
11	0.0	0.0	0.0	9.082157193976713E-5	0.0
12	0.0	0.0	0.0	9.082157193976713E-5	0.0
13	0.0	0.0	0.0	9.082157193976713E-5	0.0
14	0.0	0.0	0.0	9.082157193976713E-5	0.0
15	0.0	0.0	0.0	9.082157193976713E-5	0.0
16	0.0	0.0	0.0	9.082157193976713E-5	0.0
17	0.0	0.0	0.0	9.082157193976713E-5	0.0
18	0.0	0.0	0.0	1.8164314387953426E-4	0.0
19	0.0	0.0	0.0	1.8164314387953426E-4	0.0
20	0.0	0.0	0.0	3.632862877590685E-4	0.0
21	0.0	0.0	0.0	5.449294316386028E-4	0.0
22	0.0	0.0	0.0	9.990372913374385E-4	1.8164314387953426E-4
23	0.0	0.0	0.0	0.00127150200715674	1.8164314387953426E-4
24	0.0	0.0	0.0	0.0018164314387953426	1.8164314387953426E-4
25	0.0	0.0	0.0	0.0020888961546146442	1.8164314387953426E-4
26	0.0	0.0	0.0	0.0028154687301327813	2.724647158193014E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATGC	35	0.00206669	22.856754	3
TAGACCG	40	0.0044826264	20.000568	5
CAAGACG	470	0.0	19.403925	4
CTAGCGG	410	0.0	19.121626	29
TAACCCG	145	0.0	18.7583	28
AAGACGG	490	0.0	18.612772	5
GCGCAAG	475	0.0	18.530209	1
TCTAGCG	415	0.0	18.505709	28
GACGGAC	485	0.0	18.47475	7
CGCCCCT	235	0.0	18.383501	5
AACCCGT	150	0.0	18.133024	29
TACGCTA	410	0.0	17.951729	9
TATTGGG	45	0.008850912	17.777475	2
TAGCGGC	445	0.0	17.617678	30
CGCAAGA	500	0.0	17.599699	2
AGGGGCG	310	0.0	17.548088	32
GTACTAA	55	0.0013626766	17.458214	1
ACGGACC	525	0.0	17.371922	8
ATATACG	435	0.0	17.287846	6
CGGACCA	520	0.0	17.231256	9
>>END_MODULE