FastQCFastQC Report
Thu 2 Feb 2017
SRR4063055_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063055_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800299
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24670.3082597879042708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17020.21267051439524476No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11220.14019760114657148No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9600.11995516675642479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9560.11945535356160636No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9050.11308273532767128No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9030.11283282873026206No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8790.10983394956135145No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8290.10358628462612098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG453.535426E-421.33337415
TAACGAA659.427784E-619.69234513
CGAACGA507.193228E-419.20003916
CCGATAA507.193228E-419.2000399
CGATAAC507.193228E-419.20003910
GTCCTAG1159.313226E-1018.0994321
TTATACC450.00885745217.7744794
GATAACG550.001364136917.45457811
CGTCTTA1101.0617441E-817.45457815
ATAACGA652.0993676E-417.23080312
TCTTATA753.229921E-517.0677662
TCTAGCG1750.016.45717428
GTGTTAG1102.0011612E-716.0110381
CTAGCGG1800.016.0000329
GACCGTG703.6963468E-415.9990327
TTCCGAT703.6963468E-415.9990327
ACCGTGC703.6963468E-415.9990328
GGTTCCG805.620344E-515.9990318
ATTATAC805.6263554E-515.9970333
TACCGTC1753.6379788E-1215.5419177
Produced by FastQC (version 0.11.5)