##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063055_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800299
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229716643404526	32.0	32.0	32.0	32.0	32.0
2	30.886716089861416	32.0	32.0	32.0	32.0	32.0
3	30.940076146540232	32.0	32.0	32.0	32.0	32.0
4	30.994400842685046	32.0	32.0	32.0	32.0	32.0
5	30.93624882700091	32.0	32.0	32.0	32.0	32.0
6	34.583185784313116	36.0	36.0	36.0	32.0	36.0
7	34.51241348545981	36.0	36.0	36.0	32.0	36.0
8	34.49074533393144	36.0	36.0	36.0	32.0	36.0
9	34.60473897880667	36.0	36.0	36.0	32.0	36.0
10	34.36606693248398	36.0	36.0	36.0	32.0	36.0
11	34.60960466025823	36.0	36.0	36.0	32.0	36.0
12	34.442973188770694	36.0	36.0	36.0	32.0	36.0
13	34.522879573759305	36.0	36.0	36.0	32.0	36.0
14	34.42271950858367	36.0	36.0	36.0	32.0	36.0
15	34.37887339606822	36.0	36.0	36.0	32.0	36.0
16	34.39222840463377	36.0	36.0	36.0	32.0	36.0
17	34.318321027515964	36.0	36.0	36.0	32.0	36.0
18	34.344832368902125	36.0	36.0	36.0	32.0	36.0
19	34.3236515352387	36.0	36.0	36.0	32.0	36.0
20	34.30983419946795	36.0	36.0	36.0	32.0	36.0
21	34.28915692759831	36.0	36.0	36.0	32.0	36.0
22	34.264813525944675	36.0	36.0	36.0	32.0	36.0
23	34.204549799512435	36.0	36.0	36.0	32.0	36.0
24	34.19051004686998	36.0	36.0	36.0	32.0	36.0
25	34.174256121774484	36.0	36.0	36.0	32.0	36.0
26	34.12256669069935	36.0	36.0	36.0	32.0	36.0
27	34.12482334727395	36.0	36.0	36.0	32.0	36.0
28	34.10153954959334	36.0	36.0	36.0	32.0	36.0
29	34.067782166415306	36.0	36.0	36.0	32.0	36.0
30	34.039732649922094	36.0	36.0	36.0	32.0	36.0
31	34.05304392483309	36.0	36.0	36.0	32.0	36.0
32	34.005100593653125	36.0	36.0	36.0	32.0	36.0
33	33.96625261308586	36.0	36.0	36.0	32.0	36.0
34	33.96472943237465	36.0	36.0	36.0	32.0	36.0
35	33.91644372915623	36.0	36.0	36.0	32.0	36.0
36	33.876411191317246	36.0	36.0	36.0	32.0	36.0
37	33.867629473484286	36.0	36.0	36.0	32.0	36.0
38	33.27037019913807	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	0.0
9	3.0
10	11.0
11	8.0
12	5.0
13	1.0
14	98.0
15	214.0
16	339.0
17	354.0
18	473.0
19	604.0
20	808.0
21	1018.0
22	1640.0
23	2336.0
24	3448.0
25	5190.0
26	7417.0
27	10707.0
28	15195.0
29	20415.0
30	27478.0
31	37160.0
32	50096.0
33	74561.0
34	169109.0
35	371608.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.49978981503723	17.262065617743612	11.355492833693651	25.882651733525503
2	16.200098268793905	20.294006228691234	38.2200595863959	25.28583591611896
3	18.699927644626396	24.871378638544293	28.921280102772123	27.507413614057185
4	12.22361201530966	15.81960791174078	36.67308526151505	35.283694811434515
5	14.063528914890346	37.08823099424339	34.05373255179015	14.794507539076118
6	33.176600245658186	36.328547205481954	17.304657384302615	13.190195164557247
7	29.081916985922746	30.967214712519585	21.78013689900512	18.170731402552548
8	27.633990880885474	33.46682544105501	20.011320797154056	18.887862880905466
9	27.767850895168788	13.958835230964542	18.72832052140708	39.54499335245959
10	15.51900796705219	27.132585943201015	32.40800903665644	24.940397053090358
11	37.332761867394076	21.145322705399728	22.571071910991265	18.95084351621493
12	24.327573215640165	24.087280215626418	29.75759522063096	21.82755134810246
13	29.817389717736393	19.629730533608942	25.33661428639271	25.216265462261955
14	23.31959551325629	20.020644219618507	25.72054824649098	30.939212020634223
15	24.96481716172607	27.375622729988276	23.05694495479349	24.602615153492163
16	25.093881958043358	25.795700362531193	24.825326446781894	24.285091232643556
17	23.632798342285955	26.009138423621923	25.824420287500406	24.533642946591712
18	24.545581777785554	24.956201406053577	27.103144751541386	23.395072064619484
19	25.32927621713486	25.25779766070179	25.765270418874337	23.647655703289015
20	25.314842260216285	24.473221850390257	25.86929953689009	24.34263635250337
21	26.740219233133892	24.44004578603526	24.67284978081685	24.146885200013994
22	25.428405405912013	24.845201721994165	25.260704668003775	24.46568820409005
23	24.00045487832005	24.560430084952277	25.91019402309882	25.528921013628853
24	24.52667254863244	25.418590177783667	25.55068446586169	24.504052807722204
25	24.702965285649476	24.66522278697109	25.772127789601818	24.859684137777617
26	23.97643424183647	25.702623527765	26.422493951088803	23.898448279309726
27	24.939641598560396	25.178325960360905	25.407263002674263	24.47476943840444
28	23.89435380678183	25.2166806621881	26.06615882917466	24.822806701855406
29	24.00418894229207	25.32860452735684	26.10190926790985	24.565297262441234
30	24.0929334026431	25.449972009516763	26.27769058520103	24.179404002639103
31	24.524925553863014	25.202844635941833	25.335679287416447	24.936550522778706
32	24.15891173056184	25.61509441507142	25.10337484988935	25.122619004477393
33	23.894391418552978	24.934332036219224	25.97777161294515	25.193504932282647
34	24.608811168361473	25.322642525703305	26.037223705899482	24.031322600035743
35	25.310189513044957	25.13697329020841	25.970935667544833	23.581901529201808
36	24.09783249852534	25.839306959399337	25.454520460294134	24.60834008178119
37	24.828952219091118	25.75882731515905	25.29532688090076	24.116893584849073
38	24.160256121882824	25.67379449267973	25.612938053893565	24.55301133154388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	236.0
1	213.0
2	190.0
3	190.0
4	446.0
5	702.0
6	702.0
7	850.5
8	999.0
9	939.0
10	879.0
11	879.0
12	1146.5
13	1414.0
14	1750.0
15	2086.0
16	2086.0
17	3159.5
18	4233.0
19	4233.0
20	5105.5
21	5978.0
22	6421.5
23	6865.0
24	6865.0
25	8287.5
26	9710.0
27	9710.0
28	12508.5
29	15307.0
30	18652.0
31	21997.0
32	21997.0
33	26895.0
34	31793.0
35	31793.0
36	34516.5
37	37240.0
38	42279.5
39	47319.0
40	47319.0
41	49220.5
42	51122.0
43	56870.5
44	62619.0
45	62619.0
46	65191.5
47	67764.0
48	67764.0
49	70558.0
50	73352.0
51	72549.5
52	71747.0
53	71747.0
54	68251.5
55	64756.0
56	64756.0
57	62488.0
58	60220.0
59	53954.0
60	47688.0
61	47688.0
62	44770.0
63	41852.0
64	34734.0
65	27616.0
66	27616.0
67	22737.5
68	17859.0
69	17859.0
70	14241.0
71	10623.0
72	8285.5
73	5948.0
74	5948.0
75	4362.0
76	2776.0
77	2776.0
78	2739.5
79	2703.0
80	2095.0
81	1487.0
82	1487.0
83	1512.0
84	1537.0
85	1537.0
86	1022.0
87	507.0
88	454.0
89	401.0
90	401.0
91	294.0
92	187.0
93	157.0
94	127.0
95	127.0
96	120.0
97	113.0
98	113.0
99	230.0
100	347.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12532815860072297
2	0.05647889101448334
3	0.01024617049377795
4	0.0024990659740921828
5	2.499065974092183E-4
6	0.0
7	1.2495329870460914E-4
8	2.499065974092183E-4
9	3.7485989611382747E-4
10	3.7485989611382747E-4
11	0.002249159376682965
12	0.003248785766319838
13	0.015619162338076144
14	0.009371497402845687
15	0.024115986649989568
16	0.011495703480824042
17	0.020117481091442076
18	0.005622898441707412
19	0.007372244623571941
20	0.007122338026162722
21	0.006872431428753503
22	0.007997011117094985
23	0.010621030389891779
24	0.014119722753620835
25	0.017493461818645282
26	0.01936776129921442
27	0.009871310597664123
28	0.0053729918442981936
29	0.012120469974347087
30	0.0033737390650244474
31	0.0069973847274581134
32	0.0069973847274581134
33	0.007622151220981158
34	0.013744862857507006
35	0.01786832171475911
36	0.014369629351030052
37	0.010496077091187168
38	0.006872431428753503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	800299.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.35192010293904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.8588517964574	50.83549655237521
2	11.28579207572589	13.848100274566347
3	2.801403142304457	5.156143852883562
4	1.092237490004461	2.6804346888075345
5	0.49485223458505784	1.5180067379511657
6	0.3068586858464476	1.1295821746166474
7	0.2020366861735188	0.8676737039586204
8	0.1256947454953036	0.6169291186389699
9	0.09600362602417165	0.5301006114084676
>10	0.5752327759009216	6.819936765065641
>50	0.07643186963838484	3.2883495951326838
>100	0.0774522860651499	9.227107882067157
>500	0.0065395069978499264	2.818760215753292
>1k	6.130787810484307E-4	0.6633778267747829
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2467	0.3082597879042708	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1702	0.21267051439524476	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1122	0.14019760114657148	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	960	0.11995516675642479	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	956	0.11945535356160636	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	905	0.11308273532767128	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	903	0.11283282873026206	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	879	0.10983394956135145	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	829	0.10358628462612098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4990659740921833E-4
2	0.0	0.0	0.0	0.0	2.4990659740921833E-4
3	0.0	0.0	0.0	0.0	2.4990659740921833E-4
4	0.0	0.0	0.0	0.0	2.4990659740921833E-4
5	0.0	0.0	0.0	0.0	2.4990659740921833E-4
6	0.0	0.0	0.0	0.0	3.7485989611382747E-4
7	0.0	0.0	0.0	0.0	3.7485989611382747E-4
8	0.0	0.0	0.0	0.0	3.7485989611382747E-4
9	0.0	0.0	0.0	0.0	3.7485989611382747E-4
10	0.0	0.0	0.0	1.2495329870460916E-4	4.998131948184367E-4
11	0.0	0.0	0.0	1.2495329870460916E-4	4.998131948184367E-4
12	0.0	0.0	0.0	1.2495329870460916E-4	7.497197922276549E-4
13	0.0	0.0	0.0	1.2495329870460916E-4	8.746730909322641E-4
14	0.0	0.0	0.0	1.2495329870460916E-4	8.746730909322641E-4
15	0.0	0.0	0.0	1.2495329870460916E-4	0.0011245796883414825
16	0.0	0.0	0.0	2.4990659740921833E-4	0.0011245796883414825
17	0.0	0.0	0.0	2.4990659740921833E-4	0.0011245796883414825
18	0.0	0.0	0.0	2.4990659740921833E-4	0.0011245796883414825
19	0.0	0.0	0.0	3.7485989611382747E-4	0.0011245796883414825
20	0.0	0.0	0.0	3.7485989611382747E-4	0.0013744862857507007
21	0.0	0.0	0.0	3.7485989611382747E-4	0.0017493461818645281
22	0.0	0.0	0.0	6.247664935230458E-4	0.0018742994805691373
23	0.0	0.0	0.0	8.746730909322641E-4	0.0018742994805691373
24	0.0	0.0	0.0	0.0017493461818645281	0.0018742994805691373
25	0.0	0.0	0.0	0.002624019272796792	0.0018742994805691373
26	0.0	0.0	0.0	0.0037485989611382746	0.0019992527792737466
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAACG	45	3.535426E-4	21.333374	15
TAACGAA	65	9.427784E-6	19.692345	13
CGAACGA	50	7.193228E-4	19.200039	16
CCGATAA	50	7.193228E-4	19.200039	9
CGATAAC	50	7.193228E-4	19.200039	10
GTCCTAG	115	9.313226E-10	18.099432	1
TTATACC	45	0.008857452	17.774479	4
GATAACG	55	0.0013641369	17.454578	11
CGTCTTA	110	1.0617441E-8	17.454578	15
ATAACGA	65	2.0993676E-4	17.230803	12
TCTTATA	75	3.229921E-5	17.067766	2
TCTAGCG	175	0.0	16.457174	28
GTGTTAG	110	2.0011612E-7	16.011038	1
CTAGCGG	180	0.0	16.00003	29
GACCGTG	70	3.6963468E-4	15.999032	7
TTCCGAT	70	3.6963468E-4	15.999032	7
ACCGTGC	70	3.6963468E-4	15.999032	8
GGTTCCG	80	5.620344E-5	15.999031	8
ATTATAC	80	5.6263554E-5	15.997033	3
TACCGTC	175	3.6379788E-12	15.541917	7
>>END_MODULE