FastQCFastQC Report
Thu 2 Feb 2017
SRR4063055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800299
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26470.3307513816711004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20110.251281083694969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13200.16493835429008408No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11480.1434463869128913No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10880.13594918899061476No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10590.1323255433281811No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9020.11270787543155746No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8500.10621030389891778No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC8410.1050857242105763No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8410.1050857242105763No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8400.10496077091187168No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8100.10121217195073341No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGT400.004482361519.999964
AAATCGC1208.185452E-1118.6666314
TTAGGAC2150.018.6046143
TAGGACG2950.018.4406414
GCACTAT450.00884490517.7788561
GTATAGA1006.801747E-817.6010651
CGGCATC1750.017.37139525
CTAGGAC1201.6570993E-917.33333
ATATACT957.685376E-716.8420724
TTTATAC957.685376E-716.8420723
ATCGCTC1151.8382707E-816.6956216
AATCGCT1252.8485374E-916.63996715
TAGGACA1850.016.43244
TCGTTTA1800.015.999969530
ATCGTTT1800.015.999969529
CATCGTT1800.015.999969528
ATTTGCC2700.015.999969526
TCTAGAT1900.015.9999682
GAGCGAA2550.015.68624416
TCTAGCG1950.015.58971328
Produced by FastQC (version 0.11.5)