##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063055_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	800299
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192386845416525	32.0	32.0	32.0	32.0	32.0
2	31.307150202611773	32.0	32.0	32.0	32.0	32.0
3	31.38071270862515	32.0	32.0	32.0	32.0	32.0
4	31.48936709904673	32.0	32.0	32.0	32.0	32.0
5	31.40234962182884	32.0	32.0	32.0	32.0	32.0
6	34.928662912236554	36.0	36.0	36.0	36.0	36.0
7	34.959823765867505	36.0	36.0	36.0	36.0	36.0
8	34.898429212081986	36.0	36.0	36.0	36.0	36.0
9	35.01381983483673	36.0	36.0	36.0	36.0	36.0
10	34.86226147977194	36.0	36.0	36.0	32.0	36.0
11	35.03324507465335	36.0	36.0	36.0	36.0	36.0
12	34.9370985094321	36.0	36.0	36.0	36.0	36.0
13	34.99623390757704	36.0	36.0	36.0	36.0	36.0
14	34.939443882848785	36.0	36.0	36.0	32.0	36.0
15	34.905649013681135	36.0	36.0	36.0	32.0	36.0
16	34.9111119719005	36.0	36.0	36.0	32.0	36.0
17	34.88875532769627	36.0	36.0	36.0	32.0	36.0
18	34.880704586660734	36.0	36.0	36.0	32.0	36.0
19	34.86966621225317	36.0	36.0	36.0	32.0	36.0
20	34.85937630810485	36.0	36.0	36.0	32.0	36.0
21	34.842218970659715	36.0	36.0	36.0	32.0	36.0
22	34.82338351041298	36.0	36.0	36.0	32.0	36.0
23	34.770738186602756	36.0	36.0	36.0	32.0	36.0
24	34.748450266712815	36.0	36.0	36.0	32.0	36.0
25	34.72923994656997	36.0	36.0	36.0	32.0	36.0
26	34.66448789764826	36.0	36.0	36.0	32.0	36.0
27	34.64086797559412	36.0	36.0	36.0	32.0	36.0
28	34.60070173772552	36.0	36.0	36.0	32.0	36.0
29	34.57486639368536	36.0	36.0	36.0	32.0	36.0
30	34.54974578251379	36.0	36.0	36.0	32.0	36.0
31	34.54495757210743	36.0	36.0	36.0	32.0	36.0
32	34.517798972633976	36.0	36.0	36.0	32.0	36.0
33	34.47259461776161	36.0	36.0	36.0	32.0	36.0
34	34.45780889392589	36.0	36.0	36.0	32.0	36.0
35	34.42112385495921	36.0	36.0	36.0	32.0	36.0
36	34.38389401961017	36.0	36.0	36.0	32.0	36.0
37	34.36650052043049	36.0	36.0	36.0	32.0	36.0
38	33.94731469113419	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	6.0
21	16.0
22	69.0
23	175.0
24	517.0
25	1210.0
26	2696.0
27	5177.0
28	9221.0
29	14795.0
30	22053.0
31	32045.0
32	46611.0
33	73138.0
34	180071.0
35	412496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.796143147413645	18.49049907652473	12.072783346954282	26.64057442910734
2	15.257047678430185	21.163215248300947	37.4874890509672	26.092248022301668
3	17.61829016405119	25.487474056571358	29.43999680119555	27.454238978181905
4	11.788873380757394	16.628076826478168	36.8215799884293	34.76146980433514
5	13.486959248980693	37.63968216878941	33.80574010463589	15.067618477594
6	33.125494346488	36.72477411567427	16.99627264309964	13.15345889473809
7	28.773317905727495	31.229780943824604	21.76854342825226	18.228357722195636
8	27.38865202852987	33.71378160752933	19.995151721138996	18.902414642801805
9	27.641171104752143	13.81971084758894	18.555737438614468	39.98338060904445
10	15.287140738260149	27.291834054532938	32.598555268297496	24.822469938909418
11	37.43391218614927	20.97703124355679	22.533148560282207	19.05590801001173
12	24.110518831735178	24.18486613736383	29.63333658212316	22.071278448777832
13	29.76166378024186	19.723028172005925	25.29457776978076	25.220730277971455
14	23.271206475587842	20.00942149049479	25.800639261874952	30.91873277204241
15	24.9248093525045	27.430747758025436	22.97091462066053	24.673528268809534
16	25.073628731261692	25.804480575384947	24.627545454886235	24.494345238467123
17	23.508463711687757	25.955674066817526	25.919312656894483	24.61654956460023
18	24.628545081275874	24.81060203748849	27.125111989393965	23.435740891841675
19	25.23306914040877	25.259934099630264	25.827347029047893	23.67964973091307
20	25.418124975790295	24.33053146386538	25.905067980842162	24.346275579502162
21	26.656787025849088	24.461357567609106	24.75824660533126	24.123608801210548
22	25.377140293815188	24.851711672762306	25.386261884620627	24.384886148801886
23	23.921494145298556	24.49753029187914	25.9527400452582	25.6282355175641
24	24.376514277788676	25.477977605869807	25.68739933449873	24.458108781842785
25	24.786236144241087	24.56306955275466	25.83584385335981	24.814850449644446
26	24.027144854610587	25.662533628056515	26.38901210672511	23.921309410607787
27	24.92487164140313	24.970104848574966	25.519151015185614	24.58587249483629
28	23.891570525516087	25.082875275365836	26.17896561160266	24.846588587515416
29	24.177838532848348	24.939303935154236	26.20957916978529	24.673278362212123
30	24.162844137003795	25.15572304851062	26.401507436595573	24.279925377890013
31	24.497344117636032	25.046888725338906	25.421998528050143	25.033768628974922
32	24.188834423134352	25.43574339090765	25.27855214113725	25.096870044820747
33	23.955921474348962	24.679026213952536	26.0903737228211	25.274678588877407
34	24.51946085150675	25.060508634897705	26.242191980747197	24.177838532848348
35	25.329658040307436	24.910814583049586	25.962046685051458	23.79748069159152
36	24.161719557315454	25.585437442755772	25.689523540576708	24.56331945935207
37	24.9572972101677	25.449238347167746	25.45211227303795	24.1413521696266
38	24.19745620074497	25.372142161867	25.700519430862716	24.72988220652531
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	127.0
2	102.0
3	102.0
4	385.0
5	668.0
6	668.0
7	810.0
8	952.0
9	928.5
10	905.0
11	905.0
12	1163.5
13	1422.0
14	1747.5
15	2073.0
16	2073.0
17	3284.5
18	4496.0
19	4496.0
20	5236.5
21	5977.0
22	6600.5
23	7224.0
24	7224.0
25	8523.5
26	9823.0
27	9823.0
28	12454.5
29	15086.0
30	18577.5
31	22069.0
32	22069.0
33	26977.0
34	31885.0
35	31885.0
36	34623.5
37	37362.0
38	42232.5
39	47103.0
40	47103.0
41	49369.0
42	51635.0
43	57086.5
44	62538.0
45	62538.0
46	65525.0
47	68512.0
48	68512.0
49	71019.0
50	73526.0
51	72558.5
52	71591.0
53	71591.0
54	68313.0
55	65035.0
56	65035.0
57	62445.5
58	59856.0
59	53602.5
60	47349.0
61	47349.0
62	44689.0
63	42029.0
64	34684.5
65	27340.0
66	27340.0
67	22677.0
68	18014.0
69	18014.0
70	14331.5
71	10649.0
72	8305.0
73	5961.0
74	5961.0
75	4368.5
76	2776.0
77	2776.0
78	2726.0
79	2676.0
80	2055.5
81	1435.0
82	1435.0
83	1394.0
84	1353.0
85	1353.0
86	868.0
87	383.0
88	319.5
89	256.0
90	256.0
91	149.5
92	43.0
93	36.0
94	29.0
95	29.0
96	18.0
97	7.0
98	7.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007622151220981158
2	0.0
3	0.0
4	2.499065974092183E-4
5	0.0
6	0.0
7	2.499065974092183E-4
8	0.0018742994805691373
9	0.003623645662433665
10	0.0014994395844553099
11	0.004748225350775148
12	1.2495329870460914E-4
13	1.2495329870460914E-4
14	1.2495329870460914E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.499065974092183E-4
24	0.0
25	0.0
26	0.0
27	2.499065974092183E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	800299.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.619867359982216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74565955169687	48.73665379726431
2	12.017958329236928	14.330181630537982
3	3.005916813346282	5.3763708512053725
4	1.125530639785543	2.6841594981443997
5	0.5358072143325139	1.5972377524513022
6	0.3373682855051912	1.2068311459970467
7	0.20866651390958676	0.8708468917231614
8	0.13815329912391763	0.6589345095289535
9	0.09916774496892422	0.5321131021292227
>10	0.6140575624818935	7.060368401568675
>50	0.08090637671724593	3.3949941184512813
>100	0.0834334962493374	9.888670325935047
>500	0.006110028763649769	2.497824135199049
>1k	0.0012641438821344349	1.1648138398642587
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2647	0.3307513816711004	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2011	0.251281083694969	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1320	0.16493835429008408	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1148	0.1434463869128913	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1088	0.13594918899061476	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1059	0.1323255433281811	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	902	0.11270787543155746	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	850	0.10621030389891778	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	841	0.1050857242105763	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	841	0.1050857242105763	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	840	0.10496077091187168	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	810	0.10121217195073341	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2495329870460916E-4
2	0.0	0.0	0.0	0.0	1.2495329870460916E-4
3	0.0	0.0	0.0	0.0	1.2495329870460916E-4
4	0.0	0.0	0.0	0.0	1.2495329870460916E-4
5	0.0	0.0	0.0	0.0	1.2495329870460916E-4
6	0.0	0.0	0.0	0.0	1.2495329870460916E-4
7	0.0	0.0	0.0	0.0	1.2495329870460916E-4
8	0.0	0.0	0.0	0.0	1.2495329870460916E-4
9	0.0	0.0	0.0	0.0	1.2495329870460916E-4
10	0.0	0.0	0.0	0.0	1.2495329870460916E-4
11	0.0	0.0	0.0	0.0	1.2495329870460916E-4
12	0.0	0.0	0.0	0.0	8.746730909322641E-4
13	0.0	0.0	0.0	0.0	8.746730909322641E-4
14	0.0	0.0	0.0	0.0	8.746730909322641E-4
15	0.0	0.0	0.0	0.0	9.996263896368733E-4
16	0.0	0.0	0.0	1.2495329870460916E-4	9.996263896368733E-4
17	0.0	0.0	0.0	1.2495329870460916E-4	9.996263896368733E-4
18	0.0	0.0	0.0	1.2495329870460916E-4	9.996263896368733E-4
19	0.0	0.0	0.0	2.4990659740921833E-4	9.996263896368733E-4
20	0.0	0.0	0.0	2.4990659740921833E-4	9.996263896368733E-4
21	0.0	0.0	0.0	2.4990659740921833E-4	0.0011245796883414825
22	0.0	0.0	0.0	7.497197922276549E-4	0.0011245796883414825
23	0.0	0.0	0.0	0.0011245796883414825	0.0012495329870460916
24	0.0	0.0	0.0	0.0019992527792737466	0.0012495329870460916
25	0.0	0.0	0.0	0.0029988791689106197	0.0012495329870460916
26	0.0	0.0	0.0	0.003873552259842884	0.0012495329870460916
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGGT	40	0.0044823615	19.99996	4
AAATCGC	120	8.185452E-11	18.66663	14
TTAGGAC	215	0.0	18.604614	3
TAGGACG	295	0.0	18.440641	4
GCACTAT	45	0.008844905	17.778856	1
GTATAGA	100	6.801747E-8	17.601065	1
CGGCATC	175	0.0	17.371395	25
CTAGGAC	120	1.6570993E-9	17.3333	3
ATATACT	95	7.685376E-7	16.842072	4
TTTATAC	95	7.685376E-7	16.842072	3
ATCGCTC	115	1.8382707E-8	16.69562	16
AATCGCT	125	2.8485374E-9	16.639967	15
TAGGACA	185	0.0	16.4324	4
TCGTTTA	180	0.0	15.9999695	30
ATCGTTT	180	0.0	15.9999695	29
CATCGTT	180	0.0	15.9999695	28
ATTTGCC	270	0.0	15.9999695	26
TCTAGAT	190	0.0	15.999968	2
GAGCGAA	255	0.0	15.686244	16
TCTAGCG	195	0.0	15.589713	28
>>END_MODULE