FastQCFastQC Report
Thu 2 Feb 2017
SRR4063054_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063054_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185354
Sequences flagged as poor quality0
Sequence length38
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31911.7215706162262483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23541.2700022659343744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13220.7132298196963648No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.5492193316572612No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9300.5017426114354154No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8620.46505605490035284No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC7180.3873668763555143No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA6540.3528383525578083No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA6520.35175933618912997No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC5710.3080591732576583No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5000.26975409216957824No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG4890.2638195021418475No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4400.23738360110922885No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.20771065097057526No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3840.20717114278623605No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.20609212641755775No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA3430.18505130722833066No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA3190.1721031108041909No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG3110.16778704532947764No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3100.1672475371451385No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2980.1607734389330686No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA2810.15160179979930294No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.1510622916149638No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA2620.141351144296859No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA2620.141351144296859No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT2170.11707327600159695No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2060.11113868597386622No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA1990.10736212868349214No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT1960.10574360413047466No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC1950.1052040959461355No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA1870.10088803047142225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATGT200.0037470131.9973034
ATGGCCG200.0037470131.9973035
CTACACT350.00206105922.8552154
ATTATAC350.00206105922.85521529
AATCTGA453.5233411E-421.33153726
GAAATGT1550.020.643426
AAATGTC1600.019.9983147
TGTCCAC1600.019.99831410
TAGGACC1450.019.8603944
AATGTCC1750.019.1983838
ACTCATC507.168899E-419.19838110
AACGAAT751.4958823E-619.1983831
ACTGTTC1105.11136E-1018.9074958
TAGAAAT1700.018.8219434
ATGTCCA1700.018.8219439
GATATAC1207.8216544E-1118.6953531
TTTAGAA1750.018.3039252
ATTTAGA1700.017.9098341
TCCACTG1700.017.88084612
GTCCTAA2700.017.8050981
Produced by FastQC (version 0.11.5)