##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063054_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	185354
Sequences flagged as poor quality	0
Sequence length	38
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.783371278742298	32.0	32.0	32.0	32.0	32.0
2	30.62694088069316	32.0	32.0	32.0	32.0	32.0
3	30.765643039804914	32.0	32.0	32.0	32.0	32.0
4	30.68582819901378	32.0	32.0	32.0	32.0	32.0
5	30.832525869417438	32.0	32.0	32.0	32.0	32.0
6	34.37069067837759	36.0	36.0	36.0	32.0	36.0
7	34.11710025141081	36.0	36.0	36.0	32.0	36.0
8	34.08598141933813	36.0	36.0	36.0	32.0	36.0
9	34.070211595109896	36.0	36.0	36.0	32.0	36.0
10	34.02572914531113	36.0	36.0	36.0	32.0	36.0
11	34.28074387388457	36.0	36.0	36.0	32.0	36.0
12	34.14517086224198	36.0	36.0	36.0	32.0	36.0
13	34.18526711050207	36.0	36.0	36.0	32.0	36.0
14	34.13179645435221	36.0	36.0	36.0	32.0	36.0
15	34.19228071689847	36.0	36.0	36.0	32.0	36.0
16	34.12359053486841	36.0	36.0	36.0	32.0	36.0
17	34.09762940103801	36.0	36.0	36.0	32.0	36.0
18	34.14442634094759	36.0	36.0	36.0	32.0	36.0
19	34.040873140045534	36.0	36.0	36.0	32.0	36.0
20	33.94201905542907	36.0	36.0	36.0	32.0	36.0
21	33.89035035661491	36.0	36.0	36.0	32.0	36.0
22	33.86891030136927	36.0	36.0	36.0	32.0	36.0
23	33.88472328625225	36.0	36.0	36.0	32.0	36.0
24	33.88715107308178	36.0	36.0	36.0	32.0	36.0
25	33.86561390636296	36.0	36.0	36.0	32.0	36.0
26	33.87886962245217	36.0	36.0	36.0	32.0	36.0
27	33.83707392341142	36.0	36.0	36.0	32.0	36.0
28	33.80521596512619	36.0	36.0	36.0	27.0	36.0
29	33.718937816286676	36.0	36.0	36.0	27.0	36.0
30	33.66838050433225	36.0	36.0	36.0	27.0	36.0
31	33.65322032435232	36.0	36.0	36.0	27.0	36.0
32	33.605932431994994	36.0	36.0	36.0	27.0	36.0
33	33.54770331365927	36.0	36.0	36.0	21.0	36.0
34	33.418771647765894	36.0	36.0	36.0	21.0	36.0
35	33.36844632433074	36.0	36.0	36.0	21.0	36.0
36	33.2822652869644	36.0	36.0	36.0	21.0	36.0
37	33.23271146023285	36.0	36.0	36.0	21.0	36.0
38	32.55023360704382	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	0.0
10	2.0
11	4.0
12	1.0
13	1.0
14	139.0
15	243.0
16	280.0
17	329.0
18	395.0
19	434.0
20	526.0
21	629.0
22	787.0
23	1004.0
24	1201.0
25	1644.0
26	2119.0
27	2821.0
28	3962.0
29	5212.0
30	6870.0
31	8901.0
32	11687.0
33	16637.0
34	35908.0
35	83616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.04010631631264	18.802536897378825	12.932990470428074	21.22436631588046
2	12.032153403477706	22.1332671118621	45.78836841451761	20.046211070142576
3	18.564922731589398	31.13776655443322	29.352823102823102	20.944487611154276
4	9.818499654696131	17.516078211325965	38.426385531767956	34.23903660220994
5	9.577349288388705	43.030093766522434	33.75379004499498	13.638766900093874
6	26.624189388953027	39.04960238246814	20.877348209372336	13.44886001920649
7	25.841363013476915	34.40551593167668	21.89216310411429	17.86095795073211
8	26.985265951994304	36.621473620605	20.17447788813777	16.218782539262918
9	30.230425188694003	13.589744973105372	20.61525845279008	35.564571385410545
10	16.707579591374298	29.631028362098267	32.674949960345934	20.986442086181505
11	33.02328592701297	23.42782225867018	25.113300386300363	18.435591428016487
12	25.815223255713576	26.939594708332432	31.949090359755704	15.296091676198287
13	31.348236398659697	24.089332059936005	26.193135525087545	18.369296016316753
14	21.682430573165927	23.851968554918283	29.77948515962641	24.68611571228937
15	21.58564159017446	33.90048188740132	27.480532936167457	17.033343586256766
16	18.269178887845726	28.73406930190899	33.14448509178025	19.852266718465042
17	17.6028103196145	30.108408834591536	31.384060825504406	20.90472002028956
18	17.917051089061903	26.5901598657624	37.8223078292679	17.670481215907806
19	21.957602015765705	26.574007629263136	32.051537992543395	19.41685236242777
20	23.612152734689033	25.795695501804783	33.934747303618735	16.657404459887452
21	23.346156543883975	25.1417657183246	29.58708542632229	21.92499231146913
22	22.465738642494873	29.954677889284557	30.15431099600734	17.42527247221323
23	19.09343031035525	30.057301333793756	32.517697587085074	18.331570768765918
24	20.91123449395946	28.200355040441156	33.41373002897519	17.4746804366242
25	20.25803457728087	28.626081889015992	32.99248883037275	18.123394703330383
26	16.789967569434328	29.960770347347005	34.569040411398724	18.680221671819943
27	18.427853824613276	30.62550245765867	33.04377384144729	17.90286987628076
28	17.3236503328981	30.19660951106603	31.847071899515488	20.63266825652038
29	18.941450069875735	29.429824798061826	31.834197562173216	19.794527569889226
30	17.874234535595782	29.560009711618875	33.38207127249184	19.183684480293508
31	20.226931834122865	28.676716556420022	31.897249409200292	19.199102200256824
32	17.300543322848156	31.703724486217293	32.576709956242816	18.41902223469173
33	16.906043961973	31.38036710513537	34.18976810436922	17.523820828522407
34	17.517913411317824	31.209263176069406	34.24752881253507	17.025294600077697
35	17.965942568551913	31.873266642925746	32.96912601033809	17.19166477818425
36	17.11829836829837	31.516014849348185	33.65600448933782	17.70968229301563
37	17.955206405559544	31.643636795277843	30.672983020303334	19.72817377885928
38	17.73414695996072	34.560787297065446	30.118752798864808	17.586312944109032
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	581.0
1	447.5
2	314.0
3	314.0
4	610.5
5	907.0
6	907.0
7	1084.0
8	1261.0
9	1181.5
10	1102.0
11	1102.0
12	1332.0
13	1562.0
14	1886.5
15	2211.0
16	2211.0
17	3204.0
18	4197.0
19	4197.0
20	4655.0
21	5113.0
22	4550.0
23	3987.0
24	3987.0
25	4177.0
26	4367.0
27	4367.0
28	5844.0
29	7321.0
30	9870.5
31	12420.0
32	12420.0
33	14113.0
34	15806.0
35	15806.0
36	16257.0
37	16708.0
38	17265.5
39	17823.0
40	17823.0
41	16564.5
42	15306.0
43	14778.5
44	14251.0
45	14251.0
46	15007.5
47	15764.0
48	15764.0
49	14275.5
50	12787.0
51	10642.5
52	8498.0
53	8498.0
54	7372.5
55	6247.0
56	6247.0
57	5655.5
58	5064.0
59	4367.5
60	3671.0
61	3671.0
62	3194.5
63	2718.0
64	2320.0
65	1922.0
66	1922.0
67	1618.5
68	1315.0
69	1315.0
70	1077.5
71	840.0
72	683.0
73	526.0
74	526.0
75	433.0
76	340.0
77	340.0
78	248.0
79	156.0
80	124.0
81	92.0
82	92.0
83	71.5
84	51.0
85	51.0
86	39.0
87	27.0
88	19.5
89	12.0
90	12.0
91	8.0
92	4.0
93	4.0
94	4.0
95	4.0
96	4.5
97	5.0
98	5.0
99	39.5
100	74.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13271901334743247
2	0.0631224575676813
3	0.014027212792818067
4	0.005395081843391564
5	0.0
6	0.0
7	0.0
8	5.395081843391564E-4
9	5.395081843391564E-4
10	5.395081843391564E-4
11	0.0032370491060349383
12	0.0032370491060349383
13	0.012408688239800599
14	0.008092622765087345
15	0.022119835557905414
16	0.010790163686783129
17	0.019961802820548786
18	0.0059345900277307205
19	0.008092622765087345
20	0.0070136063964090336
21	0.0070136063964090336
22	0.00755311458074819
23	0.009711147318104817
24	0.012408688239800599
25	0.01618524553017469
26	0.018882786451870474
27	0.008092622765087345
28	0.006474098212069877
29	0.013487704608478911
30	0.004855573659052408
31	0.006474098212069877
32	0.0070136063964090336
33	0.006474098212069877
34	0.009711147318104817
35	0.010790163686783129
36	0.014027212792818067
37	0.008092622765087345
38	0.0059345900277307205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	185354.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.903257066386224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.79140090634019	44.04695939487832
2	13.048851778717758	14.5894663081366
3	3.796781958217178	6.367574335056965
4	1.5199699661245716	3.398850869977932
5	0.7274881285800773	2.0334477932378143
6	0.4386905032564267	1.4714536785675818
7	0.28779691941152324	1.1262149618143495
8	0.20985191831337252	0.9385124588277399
9	0.14090057362264452	0.7089120889225178
>10	0.895368184104958	9.414173776081908
>50	0.0789442930181348	3.0356408912421373
>100	0.0539619218098643	6.013183392221267
>500	0.005995769089984921	2.4507465200366685
>1k	0.003997179393323281	4.404863530998206
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3191	1.7215706162262483	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2354	1.2700022659343744	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1322	0.7132298196963648	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1018	0.5492193316572612	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	930	0.5017426114354154	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	862	0.46505605490035284	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	718	0.3873668763555143	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	654	0.3528383525578083	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	652	0.35175933618912997	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	571	0.3080591732576583	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	500	0.26975409216957824	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	489	0.2638195021418475	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	440	0.23738360110922885	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	385	0.20771065097057526	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	384	0.20717114278623605	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.20609212641755775	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	343	0.18505130722833066	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	319	0.1721031108041909	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	311	0.16778704532947764	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	310	0.1672475371451385	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	298	0.1607734389330686	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	281	0.15160179979930294	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	280	0.1510622916149638	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	262	0.141351144296859	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	262	0.141351144296859	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	217	0.11707327600159695	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	206	0.11113868597386622	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	199	0.10736212868349214	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	196	0.10574360413047466	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	195	0.1052040959461355	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	187	0.10088803047142225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	5.395081843391564E-4	0.0	0.0	0.0	0.0
13	5.395081843391564E-4	0.0	0.0	0.0	0.0
14	5.395081843391564E-4	0.0	0.0	0.0	0.0
15	5.395081843391564E-4	0.0	0.0	0.0	0.0
16	5.395081843391564E-4	0.0	0.0	0.0	0.0
17	5.395081843391564E-4	0.0	0.0	0.0	0.0
18	5.395081843391564E-4	0.0	0.0	5.395081843391564E-4	0.0
19	5.395081843391564E-4	0.0	0.0	5.395081843391564E-4	0.0
20	5.395081843391564E-4	0.0	0.0	5.395081843391564E-4	0.0
21	5.395081843391564E-4	0.0	0.0	0.0016185245530174694	0.0
22	5.395081843391564E-4	0.0	0.0	0.0021580327373566257	0.0
23	5.395081843391564E-4	0.0	0.0	0.009711147318104815	0.0
24	5.395081843391564E-4	0.0	0.0	0.013487704608478911	0.0
25	5.395081843391564E-4	0.0	0.0	0.01672475371451385	0.0
26	5.395081843391564E-4	0.0	0.0	0.01942229463620963	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATGT	20	0.00374701	31.997303	4
ATGGCCG	20	0.00374701	31.997303	5
CTACACT	35	0.002061059	22.855215	4
ATTATAC	35	0.002061059	22.855215	29
AATCTGA	45	3.5233411E-4	21.331537	26
GAAATGT	155	0.0	20.64342	6
AAATGTC	160	0.0	19.998314	7
TGTCCAC	160	0.0	19.998314	10
TAGGACC	145	0.0	19.860394	4
AATGTCC	175	0.0	19.198383	8
ACTCATC	50	7.168899E-4	19.198381	10
AACGAAT	75	1.4958823E-6	19.19838	31
ACTGTTC	110	5.11136E-10	18.907495	8
TAGAAAT	170	0.0	18.821943	4
ATGTCCA	170	0.0	18.821943	9
GATATAC	120	7.8216544E-11	18.695353	1
TTTAGAA	175	0.0	18.303925	2
ATTTAGA	170	0.0	17.909834	1
TCCACTG	170	0.0	17.880846	12
GTCCTAA	270	0.0	17.805098	1
>>END_MODULE