FastQCFastQC Report
Thu 2 Feb 2017
SRR4063054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185354
Sequences flagged as poor quality0
Sequence length38
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36621.975678971049991No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27351.475554884167593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17340.9355071916440973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.6053281828285335No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9060.48879441501127574No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8710.46991162855940527No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7800.420816383784542No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA6560.3539173689264866No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA5750.3102172059950149No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5580.3010455668612493No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC5270.2843208131467354No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG5200.28054425585636134No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.2681355676165607No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.25464786300808184No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4250.22929097834414147No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3880.2093291755235927No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA3750.20231556912718365No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3730.20123655275850535No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA3690.19907852002114873No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG3490.18828835633436558No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA3240.17480065172588669No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA3080.16616852077646016No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2960.1596944225643903No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2710.1462067179559114No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA2410.1300214724257367No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG2280.12300786602932766No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA2240.12084983329197103No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT2190.11815229237027525No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2180.1176127841859361No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC2160.11653376781725777No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.11491524326424031No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA2100.11329671871122284No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA1930.1041250795774572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGA200.003745464632.06
AGTGCAA200.003745464632.029
GAGCGAA200.003745464632.016
GACGGAC200.003745464632.07
CAAGACG200.003745464632.04
GGAGAGT404.9313676E-628.08
GTCCTAT356.4020554E-527.4285721
TAGGACC1400.024.04
GTATAGC350.002060048722.8571431
TTGCCAA350.002060048722.85714328
TGACTAA350.002060048722.85714325
GGACCTG3100.020.645166
GTGTAGA707.7121695E-720.5714281
AGGACCT3300.020.3636365
GTCGTAA555.799556E-520.36363630
GTGCAAG400.00446955120.01
GCCCAGT400.00446955120.019
GAACATA400.00446955120.06
TTCCTCG1052.746674E-1019.80952528
GAGGTCG507.164833E-419.227
Produced by FastQC (version 0.11.5)