##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063054_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	185354
Sequences flagged as poor quality	0
Sequence length	38
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.802836734033257	32.0	32.0	32.0	32.0	32.0
2	31.35596210494513	32.0	32.0	32.0	32.0	32.0
3	31.429534836043462	32.0	32.0	32.0	32.0	32.0
4	31.527277533800188	32.0	32.0	32.0	32.0	32.0
5	31.455911391175803	32.0	32.0	32.0	32.0	32.0
6	34.943535073427064	36.0	36.0	36.0	36.0	36.0
7	34.99091468217573	36.0	36.0	36.0	36.0	36.0
8	34.95213483388543	36.0	36.0	36.0	36.0	36.0
9	35.03001284029479	36.0	36.0	36.0	36.0	36.0
10	34.942283414439395	36.0	36.0	36.0	32.0	36.0
11	35.06703928698598	36.0	36.0	36.0	36.0	36.0
12	35.01728584222623	36.0	36.0	36.0	36.0	36.0
13	35.022999233898375	36.0	36.0	36.0	36.0	36.0
14	34.96789926303182	36.0	36.0	36.0	36.0	36.0
15	34.95253946502368	36.0	36.0	36.0	36.0	36.0
16	34.95317068959936	36.0	36.0	36.0	36.0	36.0
17	34.9381561768292	36.0	36.0	36.0	36.0	36.0
18	34.897056443346244	36.0	36.0	36.0	36.0	36.0
19	34.86974114397315	36.0	36.0	36.0	32.0	36.0
20	34.836642316863944	36.0	36.0	36.0	32.0	36.0
21	34.806699612633125	36.0	36.0	36.0	32.0	36.0
22	34.77935194276897	36.0	36.0	36.0	32.0	36.0
23	34.74289737475318	36.0	36.0	36.0	32.0	36.0
24	34.742228384604594	36.0	36.0	36.0	32.0	36.0
25	34.69936985444069	36.0	36.0	36.0	32.0	36.0
26	34.67420719272312	36.0	36.0	36.0	32.0	36.0
27	34.60178900913927	36.0	36.0	36.0	32.0	36.0
28	34.54596070222385	36.0	36.0	36.0	32.0	36.0
29	34.49995683934525	36.0	36.0	36.0	32.0	36.0
30	34.44076200135956	36.0	36.0	36.0	32.0	36.0
31	34.39196888116793	36.0	36.0	36.0	32.0	36.0
32	34.313130550190444	36.0	36.0	36.0	32.0	36.0
33	34.26547579226777	36.0	36.0	36.0	32.0	36.0
34	34.153921684991964	36.0	36.0	36.0	32.0	36.0
35	34.06505389686762	36.0	36.0	36.0	32.0	36.0
36	34.0021634278192	36.0	36.0	36.0	32.0	36.0
37	33.92559642629779	36.0	36.0	36.0	32.0	36.0
38	33.53734475651996	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	1.0
21	10.0
22	19.0
23	47.0
24	135.0
25	294.0
26	671.0
27	1339.0
28	2442.0
29	3870.0
30	5854.0
31	8215.0
32	11441.0
33	16918.0
34	38819.0
35	95276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.563024031250336	20.08341336556204	13.533359950793669	21.820202652393952
2	11.90802464473386	22.956073243631106	44.46680406141761	20.66909805021742
3	18.197611057759747	31.2256546931817	30.040894720372908	20.53583952868565
4	9.655577975117883	18.145818272063188	38.521963378184445	33.67664037463448
5	9.33726814635778	43.13961392794329	33.73814430764915	13.784973618049786
6	26.994977151705125	39.214363943394495	20.119447756443112	13.67121114845727
7	25.218770568749527	34.49615330664566	22.360995716305016	17.924080408299794
8	26.9521070833176	36.624026846361765	20.047369585273348	16.37649648504729
9	29.314809819260855	13.343943889937956	21.16374426760183	36.17750202319935
10	16.654617650549756	29.60713014016423	32.88949793368366	20.84875427560236
11	32.740388246846436	22.890162183160143	25.646088936367654	18.72336063362576
12	25.480432038154017	26.999147577068744	32.07430106714719	15.446119317630046
13	31.29471174077711	23.490725854311208	26.871823645564703	18.34273875934698
14	21.570076718063813	23.75346634008438	29.722584891612808	24.953872050239
15	21.80152572914531	33.800727257032484	27.491718549370393	16.906028464451804
16	18.36863515219526	28.398092299060178	32.971503177703205	20.261769371041357
17	17.54318763015635	29.889832428757945	31.631904355989082	20.935075585096627
18	18.174412205833164	25.642284493455765	38.18477076297248	17.998532537738598
19	22.2450014566721	25.941172027579658	32.917012851084955	18.89681366466329
20	23.629379457686372	25.270023846261747	34.48536314295888	16.615233553093
21	23.236203352521944	24.751150507410184	30.35343371836442	21.65921242170345
22	22.386892109153294	29.02607982563096	31.179796497512868	17.407231567702883
23	18.944290384885136	29.329283425229562	33.3103143174682	18.416111872417105
24	20.991723944452236	27.68378346299513	33.74084184857084	17.583650743981785
25	20.31248314036924	27.87315083569818	33.54068431218102	18.273681711751564
26	16.82779977772263	29.268319000399234	35.13978657056228	18.76409465131586
27	18.867140714524638	29.674568663206617	33.663152670026	17.795137952242733
28	17.570702547557644	29.286122770482425	32.92510547385004	20.21806920810989
29	18.856890059022195	28.415896069143372	32.845258262567846	19.881955609266594
30	18.27206318719855	28.53404836151364	33.8999967629509	19.293891688336913
31	20.001186918005544	27.275375767450395	33.03192809434919	19.69150922019487
32	17.559372875686524	30.404523236617496	33.46569267455787	18.5704112131381
33	17.222180260474552	30.381324384690917	34.64236002460157	17.75413533023296
34	17.814560246878944	30.07164668688024	34.877585592973446	17.23620747326737
35	18.279616301779296	30.668342738759346	33.4840359528254	17.56800500663595
36	17.56153090842388	30.327373566257	34.1012333157094	18.00986220960972
37	18.18304433678259	30.700713229819698	31.395060263064188	19.721182170333524
38	18.256956958037055	32.83608662343408	31.12045059723556	17.786505821293307
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	329.0
1	242.0
2	155.0
3	155.0
4	523.0
5	891.0
6	891.0
7	1049.0
8	1207.0
9	1210.5
10	1214.0
11	1214.0
12	1446.5
13	1679.0
14	1966.5
15	2254.0
16	2254.0
17	3363.0
18	4472.0
19	4472.0
20	4901.0
21	5330.0
22	4738.0
23	4146.0
24	4146.0
25	4160.5
26	4175.0
27	4175.0
28	5686.5
29	7198.0
30	9583.5
31	11969.0
32	11969.0
33	13789.0
34	15609.0
35	15609.0
36	16014.5
37	16420.0
38	17017.5
39	17615.0
40	17615.0
41	16446.0
42	15277.0
43	14692.0
44	14107.0
45	14107.0
46	15145.5
47	16184.0
48	16184.0
49	14792.0
50	13400.0
51	11090.5
52	8781.0
53	8781.0
54	7598.5
55	6416.0
56	6416.0
57	5741.5
58	5067.0
59	4359.5
60	3652.0
61	3652.0
62	3160.0
63	2668.0
64	2252.5
65	1837.0
66	1837.0
67	1587.0
68	1337.0
69	1337.0
70	1076.5
71	816.0
72	644.0
73	472.0
74	472.0
75	396.0
76	320.0
77	320.0
78	253.5
79	187.0
80	140.0
81	93.0
82	93.0
83	68.0
84	43.0
85	43.0
86	31.5
87	20.0
88	13.0
89	6.0
90	6.0
91	3.0
92	0.0
93	2.0
94	4.0
95	4.0
96	3.5
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006474098212069877
2	0.0
3	0.0
4	0.0
5	0.0
6	5.395081843391564E-4
7	0.0
8	0.0016185245530174691
9	0.0021580327373566257
10	0.0
11	0.004316065474713251
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	5.395081843391564E-4
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	185354.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.46443803142385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16997074303762	42.57483527452388
2	13.376994993342898	14.571410297231827
3	3.8830356441690297	6.3446206264696245
4	1.6109997234651576	3.5096877842923617
5	0.7959997054980112	2.167683831656403
6	0.44299983423716693	1.4476642211844728
7	0.31099988360153025	1.1856903721736902
8	0.22499991578846337	0.980359517642908
9	0.13099995097016678	0.642135484056079
>10	0.9039996616567226	9.40872814848919
>50	0.07699997118093765	2.916024920811064
>100	0.05999997754358779	6.274300912914354
>500	0.007999997005811705	2.937266043693326
>1k	0.003999998502905853	5.0395925648608095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3662	1.975678971049991	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2735	1.475554884167593	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1734	0.9355071916440973	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1122	0.6053281828285335	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	906	0.48879441501127574	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	871	0.46991162855940527	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	780	0.420816383784542	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	656	0.3539173689264866	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	575	0.3102172059950149	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	558	0.3010455668612493	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	527	0.2843208131467354	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	520	0.28054425585636134	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	497	0.2681355676165607	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	472	0.25464786300808184	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	425	0.22929097834414147	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	388	0.2093291755235927	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	375	0.20231556912718365	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	373	0.20123655275850535	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	369	0.19907852002114873	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	349	0.18828835633436558	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	324	0.17480065172588669	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	308	0.16616852077646016	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	296	0.1596944225643903	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	271	0.1462067179559114	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	241	0.1300214724257367	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	228	0.12300786602932766	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	224	0.12084983329197103	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	219	0.11815229237027525	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	218	0.1176127841859361	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	216	0.11653376781725777	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	213	0.11491524326424031	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	210	0.11329671871122284	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	193	0.1041250795774572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	5.395081843391564E-4	0.0
19	0.0	0.0	0.0	5.395081843391564E-4	0.0
20	0.0	0.0	0.0	0.0010790163686783128	0.0
21	0.0	0.0	0.0	0.0021580327373566257	0.0
22	0.0	0.0	0.0	0.002697540921695782	0.0
23	0.0	0.0	0.0	0.009711147318104815	0.0
24	0.0	0.0	0.0	0.013487704608478911	0.0
25	0.0	0.0	0.0	0.01672475371451385	0.0
26	0.0	0.0	0.0	0.01942229463620963	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGGA	20	0.0037454646	32.0	6
AGTGCAA	20	0.0037454646	32.0	29
GAGCGAA	20	0.0037454646	32.0	16
GACGGAC	20	0.0037454646	32.0	7
CAAGACG	20	0.0037454646	32.0	4
GGAGAGT	40	4.9313676E-6	28.0	8
GTCCTAT	35	6.4020554E-5	27.428572	1
TAGGACC	140	0.0	24.0	4
GTATAGC	35	0.0020600487	22.857143	1
TTGCCAA	35	0.0020600487	22.857143	28
TGACTAA	35	0.0020600487	22.857143	25
GGACCTG	310	0.0	20.64516	6
GTGTAGA	70	7.7121695E-7	20.571428	1
AGGACCT	330	0.0	20.363636	5
GTCGTAA	55	5.799556E-5	20.363636	30
GTGCAAG	40	0.004469551	20.0	1
GCCCAGT	40	0.004469551	20.0	19
GAACATA	40	0.004469551	20.0	6
TTCCTCG	105	2.746674E-10	19.809525	28
GAGGTCG	50	7.164833E-4	19.2	27
>>END_MODULE