FastQCFastQC Report
Thu 2 Feb 2017
SRR4063053_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063053_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244202
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21290.17111369375712304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20080.16138858481179102No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17820.1432243317403444No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16900.13583003402984403No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16470.1323740035781971No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15820.12714977150012619No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15770.12674790749412074No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14970.12031808339803343No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14930.11999659219322908No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14660.1178265265607996No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14170.11388825930194615No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13820.11107521125990796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT13250.10649396159144577No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13220.10625284318784248No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13030.10472575996502174No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA12920.10384165915180976No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12820.10303793113979884No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT12590.10118935671217374No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG12550.1008678655073694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA2750.020.94466630
GTATCAA14200.019.9693981
GTTCTAA902.00871E-819.5807931
ACGATTT507.198218E-419.19927631
CATCGTT3050.018.88377628
ATCGTTT3150.018.79294229
CGACGCT701.7874134E-518.2857615
TCTATAC1159.440555E-1018.0833663
CCGATAA2250.017.7785389
TAACGAA2250.017.77853813
CGCACGA450.008854642517.77639428
TCTAGCG3650.017.5328828
GTACGCC550.001366915417.450384
ATAACGA2300.017.39204812
CTAGCGG3800.017.26250629
CTATACA1404.0017767E-1117.1387654
CGTTTAT3400.016.94053831
ACGAACG2450.016.3265715
TAGTACG600.002434817616.0071182
GGTCGTA703.6977633E-415.99939729
Produced by FastQC (version 0.11.5)