##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063053_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1244202
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.746609473381334	32.0	32.0	32.0	32.0	32.0
2	30.122355533908483	32.0	32.0	32.0	21.0	32.0
3	30.183743475737863	32.0	32.0	32.0	21.0	32.0
4	30.052124976490955	32.0	32.0	32.0	21.0	32.0
5	30.077439997685264	32.0	32.0	32.0	21.0	32.0
6	33.39677238904937	36.0	36.0	36.0	21.0	36.0
7	33.421149459653655	36.0	36.0	36.0	21.0	36.0
8	33.30601783311713	36.0	36.0	36.0	21.0	36.0
9	33.57399682688181	36.0	36.0	36.0	21.0	36.0
10	32.894755031739216	36.0	32.0	36.0	21.0	36.0
11	33.70769055185573	36.0	36.0	36.0	27.0	36.0
12	33.233983710040654	36.0	32.0	36.0	21.0	36.0
13	33.48926139003152	36.0	36.0	36.0	21.0	36.0
14	33.15683948426381	36.0	32.0	36.0	21.0	36.0
15	33.066256926126144	36.0	32.0	36.0	21.0	36.0
16	33.13921372896041	36.0	32.0	36.0	21.0	36.0
17	32.97739514966219	36.0	32.0	36.0	21.0	36.0
18	33.06059064364147	36.0	32.0	36.0	21.0	36.0
19	32.99975647041236	36.0	32.0	36.0	21.0	36.0
20	32.9742638253274	36.0	32.0	36.0	21.0	36.0
21	32.96168226702738	36.0	32.0	36.0	21.0	36.0
22	32.90938690019788	36.0	32.0	36.0	21.0	36.0
23	32.87366440497604	36.0	32.0	36.0	21.0	36.0
24	32.85828024709814	36.0	32.0	36.0	21.0	36.0
25	32.83739376725001	36.0	32.0	36.0	21.0	36.0
26	32.786466345496954	36.0	32.0	36.0	21.0	36.0
27	32.79741312102054	36.0	32.0	36.0	21.0	36.0
28	32.75292918673977	36.0	32.0	36.0	21.0	36.0
29	32.718985341608516	36.0	32.0	36.0	21.0	36.0
30	32.68110322921841	36.0	32.0	36.0	21.0	36.0
31	32.69878685293867	36.0	32.0	36.0	21.0	36.0
32	32.683699270697204	36.0	32.0	36.0	21.0	36.0
33	32.59934881956467	36.0	32.0	36.0	14.0	36.0
34	32.64393804221501	36.0	32.0	36.0	14.0	36.0
35	32.642165821948524	36.0	32.0	36.0	14.0	36.0
36	32.581722260533255	36.0	32.0	36.0	14.0	36.0
37	32.63659357564126	36.0	32.0	36.0	14.0	36.0
38	31.59661132195576	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	4.0
9	2.0
10	7.0
11	5.0
12	8.0
13	7.0
14	191.0
15	426.0
16	648.0
17	1061.0
18	1680.0
19	2535.0
20	3443.0
21	4710.0
22	6348.0
23	8583.0
24	11545.0
25	16078.0
26	22136.0
27	30982.0
28	41790.0
29	56357.0
30	74743.0
31	99100.0
32	133729.0
33	184926.0
34	285193.0
35	257958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.066544138651935	17.273553180994103	11.343363571037147	26.31653910931682
2	17.12229348022758	19.252206429303794	36.888442130234615	26.73705796023401
3	18.900856871855055	22.7519573010948	28.629165795861937	29.718020031188207
4	12.761830970180144	14.705936724131972	35.658362074023586	36.8738702316643
5	14.94702222550858	35.875393123779844	33.7571662456468	15.420418405064776
6	35.228580614049186	35.13237421636393	16.154476772223113	13.48456839736377
7	30.817182418931978	30.033949471227338	20.544011342209707	18.604856767630977
8	28.514788300528938	32.64983808782078	19.20409454160247	19.631279070047814
9	27.210737823501045	13.920511171837324	18.53504259765311	40.333708407008515
10	16.002568731242878	26.041999881047488	31.324616579086538	26.630814808623093
11	38.380156600883154	20.756931059925314	21.65768352218566	19.205228817005874
12	24.467377981393295	23.526915425884624	28.35477324331384	23.65093334940824
13	29.34959976978121	18.704874625613126	25.277283418298857	26.668242186306806
14	23.503756533844975	19.45962499768909	24.519190193063416	32.51742827540252
15	25.262069675162223	26.946229090698615	21.82083759397529	25.970863640163866
16	26.315006820295462	25.413055902308614	23.14198191275327	25.129955364642655
17	24.44047157783719	25.73830741948916	24.684202656926	25.13701834574765
18	25.616306780962027	24.308340265805032	25.556345924309465	24.51900702892348
19	25.748903440908787	24.756233125609366	24.994232866791684	24.500630566690166
20	25.882501402582037	23.854424041140263	24.484179356885992	25.778895199391705
21	27.171217775648664	24.009327007086885	24.035851587737284	24.783603629527164
22	26.034326298368565	23.79306159619779	24.82101792513417	25.351594180299475
23	24.494471072500207	23.54992753757155	25.46127982393599	26.49432156599225
24	24.946284615576296	24.67362829838183	24.79508575571698	25.585001330324896
25	25.1456178005	24.150770492198614	24.892886713129318	25.810724994172073
26	24.903054038932726	25.126126213030307	25.31648113492691	24.654338613110053
27	25.782654127481713	24.17876376497066	24.468049192187124	25.5705329153605
28	24.75117250137646	24.248172425018186	25.509450340999972	25.491204732605382
29	24.611138038661533	24.644174525894254	25.45087570805391	25.293811727390302
30	24.625615668522784	24.803564768971594	25.563103612350663	25.007715950154964
31	25.46389846720198	24.509255463657333	24.27045405222927	25.756392016911416
32	25.085863420695798	24.38505072231372	24.266172183386423	26.26291367360406
33	24.6238046589465	24.050860822393037	24.988967937490607	26.336366581169855
34	25.647726286208528	24.19649144242487	25.010690940191825	25.14509133117478
35	26.109041625335006	23.92070109212043	25.239750418407684	24.730506864136874
36	24.618460207807157	24.833244643652364	24.775529364858627	25.77276578368185
37	25.919751528431313	24.448229463236466	24.640338368890895	24.991680639441324
38	24.70286489123734	24.21794810996591	25.331817951646574	25.74736904715018
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	38.0
2	60.0
3	60.0
4	215.0
5	370.0
6	370.0
7	434.5
8	499.0
9	454.5
10	410.0
11	410.0
12	625.0
13	840.0
14	1263.0
15	1686.0
16	1686.0
17	2684.0
18	3682.0
19	3682.0
20	4733.5
21	5785.0
22	6782.0
23	7779.0
24	7779.0
25	9416.0
26	11053.0
27	11053.0
28	13823.5
29	16594.0
30	20560.5
31	24527.0
32	24527.0
33	32248.0
34	39969.0
35	39969.0
36	45248.0
37	50527.0
38	58342.5
39	66158.0
40	66158.0
41	71193.5
42	76229.0
43	87120.0
44	98011.0
45	98011.0
46	99467.5
47	100924.0
48	100924.0
49	108512.5
50	116101.0
51	119013.5
52	121926.0
53	121926.0
54	115823.0
55	109720.0
56	109720.0
57	106142.0
58	102564.0
59	91744.5
60	80925.0
61	80925.0
62	76959.0
63	72993.0
64	60891.5
65	48790.0
66	48790.0
67	41351.0
68	33912.0
69	33912.0
70	27205.0
71	20498.0
72	16003.0
73	11508.0
74	11508.0
75	8873.0
76	6238.0
77	6238.0
78	5937.5
79	5637.0
80	4345.0
81	3053.0
82	3053.0
83	3129.0
84	3205.0
85	3205.0
86	1946.0
87	687.0
88	625.5
89	564.0
90	564.0
91	357.0
92	150.0
93	122.0
94	94.0
95	94.0
96	80.5
97	67.0
98	67.0
99	259.0
100	451.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11999659219322908
2	0.0544123864131387
3	0.011412937770554943
4	0.0024915568372338255
5	8.037280120109113E-5
6	1.6074560240218226E-4
7	0.0
8	7.233552108098203E-4
9	1.6074560240218226E-4
10	6.42982409608729E-4
11	0.0016074560240218229
12	0.002170065632429461
13	0.013502630601783312
14	0.008278398523712387
15	0.020736182709881514
16	0.009725108945332028
17	0.016717542649826958
18	0.004581249668462195
19	0.006670942499690564
20	0.005465350481674198
21	0.005867214487679653
22	0.006670942499690564
23	0.008760635330918934
24	0.011654056174158215
25	0.0154315778306095
26	0.016878288252229142
27	0.008198025722511297
28	0.005384977680473107
29	0.009885854547734212
30	0.0020896928312283698
31	0.005786841686478562
32	0.006027960090081836
33	0.008117652921310205
34	0.0126989025897724
35	0.01607456024021823
36	0.013181139396978948
37	0.009323244939326573
38	0.005706468885277471
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1244202.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.16016559271511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.24475977342357	49.83933938514243
2	9.892655278729958	11.70502248882623
3	2.5593674933261914	4.54237814153369
4	1.0583332268343804	2.504446758071779
5	0.552799756757562	1.6351862574695002
6	0.364828532892252	1.2949989851311765
7	0.24246181880137335	1.0040856945140093
8	0.16675347223855488	0.7892130424634504
9	0.12788174548497352	0.6808954715258833
>10	0.6400118496037125	6.8425579458838754
>50	0.06283898914268113	2.645460788253558
>100	0.07355126448482077	9.456590172052227
>500	0.010351650190907745	4.217780616175541
>1k	0.0034051480891143897	2.842044252956677
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2129	0.17111369375712304	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2008	0.16138858481179102	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1782	0.1432243317403444	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1690	0.13583003402984403	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1647	0.1323740035781971	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1582	0.12714977150012619	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1577	0.12674790749412074	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1497	0.12031808339803343	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1493	0.11999659219322908	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1466	0.1178265265607996	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1417	0.11388825930194615	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1382	0.11107521125990796	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1325	0.10649396159144577	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1322	0.10625284318784248	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1303	0.10472575996502174	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1292	0.10384165915180976	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1282	0.10303793113979884	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1259	0.10118935671217374	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1255	0.1008678655073694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.037280120109114E-5
2	0.0	0.0	0.0	0.0	8.037280120109114E-5
3	0.0	0.0	0.0	0.0	8.037280120109114E-5
4	0.0	0.0	0.0	0.0	8.037280120109114E-5
5	0.0	0.0	0.0	0.0	8.037280120109114E-5
6	0.0	0.0	0.0	0.0	8.037280120109114E-5
7	0.0	0.0	0.0	0.0	8.037280120109114E-5
8	0.0	0.0	0.0	0.0	8.037280120109114E-5
9	0.0	0.0	0.0	0.0	8.037280120109114E-5
10	0.0	0.0	0.0	0.0	8.037280120109114E-5
11	0.0	0.0	0.0	0.0	8.037280120109114E-5
12	0.0	0.0	0.0	0.0	2.4111840360327343E-4
13	0.0	0.0	0.0	0.0	2.4111840360327343E-4
14	0.0	0.0	0.0	0.0	2.4111840360327343E-4
15	0.0	0.0	0.0	0.0	2.4111840360327343E-4
16	0.0	0.0	0.0	0.0	3.214912048043646E-4
17	0.0	0.0	0.0	0.0	3.214912048043646E-4
18	0.0	0.0	0.0	0.0	3.214912048043646E-4
19	0.0	0.0	0.0	0.0	3.214912048043646E-4
20	0.0	0.0	0.0	0.0	3.214912048043646E-4
21	0.0	0.0	0.0	0.0	3.214912048043646E-4
22	8.037280120109114E-5	0.0	0.0	0.0	3.214912048043646E-4
23	8.037280120109114E-5	0.0	0.0	1.607456024021823E-4	3.214912048043646E-4
24	8.037280120109114E-5	0.0	0.0	6.429824096087292E-4	3.214912048043646E-4
25	8.037280120109114E-5	0.0	0.0	9.644736144130937E-4	3.214912048043646E-4
26	8.037280120109114E-5	0.0	0.0	0.0012055920180163672	3.214912048043646E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	275	0.0	20.944666	30
GTATCAA	1420	0.0	19.969398	1
GTTCTAA	90	2.00871E-8	19.580793	1
ACGATTT	50	7.198218E-4	19.199276	31
CATCGTT	305	0.0	18.883776	28
ATCGTTT	315	0.0	18.792942	29
CGACGCT	70	1.7874134E-5	18.28576	15
TCTATAC	115	9.440555E-10	18.083366	3
CCGATAA	225	0.0	17.778538	9
TAACGAA	225	0.0	17.778538	13
CGCACGA	45	0.0088546425	17.776394	28
TCTAGCG	365	0.0	17.53288	28
GTACGCC	55	0.0013669154	17.45038	4
ATAACGA	230	0.0	17.392048	12
CTAGCGG	380	0.0	17.262506	29
CTATACA	140	4.0017767E-11	17.138765	4
CGTTTAT	340	0.0	16.940538	31
ACGAACG	245	0.0	16.32657	15
TAGTACG	60	0.0024348176	16.007118	2
GGTCGTA	70	3.6977633E-4	15.999397	29
>>END_MODULE