FastQCFastQC Report
Thu 2 Feb 2017
SRR4063053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244202
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20980.1686221369198892No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20060.16122783920938882No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17220.13840196366827895No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16790.134945933216632No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16220.13036468354816982No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15870.12755163550613166No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15070.12112181141004436No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14760.11863025457281053No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14580.11718354415119088No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14540.1168620529463865No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14290.11485273291635924No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13440.1080210448142665No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13330.10713694400105449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT12640.1015912207181792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG400.004482250720.0011049
TCTAGCG4150.018.89109228
GGTATCA6000.018.4039761
AAGACGG4450.018.3380935
CTAGCGG4400.018.1813629
CAAGACG4650.017.2052524
GTATCAA12500.017.1557061
CGCTTCG3550.017.1263332
TAGCGGC4700.017.0208530
TTTTCGG3950.017.01223229
GCGCAAG4750.016.8457451
TAAACGC3650.016.65711628
GTTTTCG4100.016.38983228
CGACCCG2650.016.3027885
AACCGGT600.00244115716.0008857
TAGGATA1001.3138233E-616.0008854
ACGCTTC3900.015.99959831
AACGCTT3750.015.7862730
GCGTAAC2450.015.67307611
ATAACGA2150.015.62751512
Produced by FastQC (version 0.11.5)