##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1244202
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.917169398538178	32.0	32.0	32.0	32.0	32.0
2	30.64501343029508	32.0	32.0	32.0	32.0	32.0
3	31.006932957831605	32.0	32.0	32.0	32.0	32.0
4	31.215943231083056	32.0	32.0	32.0	32.0	32.0
5	31.001825266315276	32.0	32.0	32.0	32.0	32.0
6	34.55752683245968	36.0	36.0	36.0	32.0	36.0
7	34.59806928456955	36.0	36.0	36.0	32.0	36.0
8	34.49922199128437	36.0	36.0	36.0	32.0	36.0
9	34.72024639085936	36.0	36.0	36.0	32.0	36.0
10	34.40508615160561	36.0	36.0	36.0	32.0	36.0
11	34.74570929800788	36.0	36.0	36.0	32.0	36.0
12	34.56862551257754	36.0	36.0	36.0	32.0	36.0
13	34.67992174904075	36.0	36.0	36.0	32.0	36.0
14	34.60365278306899	36.0	36.0	36.0	32.0	36.0
15	34.525726529936456	36.0	36.0	36.0	32.0	36.0
16	34.572328287528876	36.0	36.0	36.0	32.0	36.0
17	34.51002007712574	36.0	36.0	36.0	32.0	36.0
18	34.53249633098162	36.0	36.0	36.0	32.0	36.0
19	34.52728656600777	36.0	36.0	36.0	32.0	36.0
20	34.51561241663331	36.0	36.0	36.0	32.0	36.0
21	34.51459489697011	36.0	36.0	36.0	32.0	36.0
22	34.49696512302665	36.0	36.0	36.0	32.0	36.0
23	34.41931454860224	36.0	36.0	36.0	32.0	36.0
24	34.3863022242369	36.0	36.0	36.0	32.0	36.0
25	34.36639950747547	36.0	36.0	36.0	32.0	36.0
26	34.281430185773694	36.0	36.0	36.0	32.0	36.0
27	34.27549706558903	36.0	36.0	36.0	32.0	36.0
28	34.23783517467421	36.0	36.0	36.0	32.0	36.0
29	34.19174298064141	36.0	36.0	36.0	32.0	36.0
30	34.1695994701825	36.0	36.0	36.0	32.0	36.0
31	34.17679122843397	36.0	36.0	36.0	32.0	36.0
32	34.130441841437325	36.0	36.0	36.0	32.0	36.0
33	34.09422907212816	36.0	36.0	36.0	32.0	36.0
34	34.09908037440866	36.0	36.0	36.0	32.0	36.0
35	34.07187900357016	36.0	36.0	36.0	32.0	36.0
36	34.02112357961167	36.0	36.0	36.0	32.0	36.0
37	34.04180993118481	36.0	36.0	36.0	32.0	36.0
38	33.48586403172475	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	26.0
22	73.0
23	318.0
24	833.0
25	2136.0
26	4807.0
27	9642.0
28	17152.0
29	28501.0
30	44930.0
31	68018.0
32	102809.0
33	167327.0
34	348141.0
35	449481.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.75320004018889	18.974299206269464	12.29185170300412	26.980649050537526
2	16.214328541506926	20.55405794235984	35.6802995012064	27.551314014926838
3	18.129612394128927	23.813898386274897	28.83462653170466	29.221862687891516
4	12.469619032309918	15.899212345282109	35.852274553930236	35.77889406847773
5	15.033447221867242	36.228529359049475	32.69083161134191	16.047191807741363
6	35.47690445514832	35.805066866205706	15.539129127849922	13.178899550796054
7	30.68364575706259	30.328235801886677	20.336329375492788	18.651789065557946
8	28.506509111631047	33.02265992272816	18.62850924183739	19.84232172380341
9	27.26862814126089	13.647375028734107	18.02130905361503	41.062687776389964
10	15.710191241502477	26.38295238814775	31.191477801550572	26.715378568799196
11	38.84518190820355	20.557588189148525	21.32606836507671	19.271161537571214
12	24.516333854419926	23.496236522410072	28.077029726047765	23.910399897122236
13	29.564997938435994	18.582769182792813	25.069502435699697	26.782730443071497
14	23.5490700063173	19.185711001911265	24.37015854338765	32.89506044838378
15	25.440643882584986	26.966682258990097	21.491365549966968	26.10130830845795
16	26.43919556470734	25.53178663914702	22.763024010570632	25.265993785575013
17	24.667457535030486	25.898929595033604	24.220584760352416	25.21302810958349
18	25.84998255910214	24.38711720444108	25.170510897748116	24.592389338708667
19	25.92593485623717	24.795009170536616	24.667055671024478	24.612000302201732
20	26.126143504029088	23.825472069647855	24.152589370536294	25.895795055786763
21	27.293368568939297	24.02467135403104	23.766110805692993	24.915849271336672
22	26.18208297366505	23.951416249129963	24.436224985974945	25.430275791230038
23	24.603922199003375	23.5326314097412	25.18453624819161	26.678910143063817
24	25.082100816426916	24.671878039096544	24.552363683710524	25.69365746076602
25	25.347914042827487	24.084613338198572	24.67615763047932	25.891314988494624
26	25.275075912110733	25.04111068781436	24.780943930326426	24.90286946974848
27	26.082843527693676	24.108725197938274	24.106956994890698	25.70147427947735
28	24.987662775015632	24.19808037601611	25.258358369460908	25.55589847950735
29	24.894269580019966	24.501728818953836	25.14945322383343	25.454548377192772
30	24.883097760653015	24.65162409319387	25.264064838346183	25.20121330780693
31	25.622206040498245	24.433974547541315	24.061044749968254	25.882774661992187
32	25.45519135960238	24.321291880257387	24.037736637619936	26.185780122520296
33	24.844518816076487	23.992647496146123	24.803609060265135	26.35922462751225
34	25.757553837720888	24.09046119520785	24.86975587565363	25.28222909141763
35	26.316787788478074	23.93775287292578	24.844518816076487	24.900940522519655
36	25.044727463868405	24.796054016952233	24.395475975765994	25.763742543413372
37	26.248390534655947	24.41966818892752	24.28407927330128	25.047862003115252
38	25.086762438896574	23.89218149464476	24.981956306130353	26.039099760328305
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	12.0
2	18.0
3	18.0
4	77.5
5	137.0
6	137.0
7	204.5
8	272.0
9	294.0
10	316.0
11	316.0
12	493.0
13	670.0
14	1097.0
15	1524.0
16	1524.0
17	2412.0
18	3300.0
19	3300.0
20	4195.0
21	5090.0
22	6384.5
23	7679.0
24	7679.0
25	9539.5
26	11400.0
27	11400.0
28	14188.0
29	16976.0
30	20718.5
31	24461.0
32	24461.0
33	32166.5
34	39872.0
35	39872.0
36	44950.5
37	50029.0
38	57753.5
39	65478.0
40	65478.0
41	70425.5
42	75373.0
43	86275.5
44	97178.0
45	97178.0
46	98551.5
47	99925.0
48	99925.0
49	107228.5
50	114532.0
51	117224.0
52	119916.0
53	119916.0
54	114727.5
55	109539.0
56	109539.0
57	106768.0
58	103997.0
59	93390.5
60	82784.0
61	82784.0
62	78709.5
63	74635.0
64	62483.0
65	50331.0
66	50331.0
67	42752.0
68	35173.0
69	35173.0
70	28357.5
71	21542.0
72	16874.5
73	12207.0
74	12207.0
75	9309.0
76	6411.0
77	6411.0
78	6003.5
79	5596.0
80	4493.0
81	3390.0
82	3390.0
83	3067.0
84	2744.0
85	2744.0
86	1847.5
87	951.0
88	744.0
89	537.0
90	537.0
91	322.0
92	107.0
93	80.5
94	54.0
95	54.0
96	39.0
97	24.0
98	24.0
99	26.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006188705692484018
2	0.0
3	0.0
4	1.6074560240218226E-4
5	2.4111840360327343E-4
6	8.037280120109113E-5
7	4.018640060054557E-4
8	0.001848574427625096
9	0.002893420843239281
10	0.001285964819217458
11	0.004661622469663286
12	5.626096084076379E-4
13	8.037280120109113E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	4.8223680720654687E-4
22	0.0
23	1.6074560240218226E-4
24	0.0
25	8.037280120109113E-5
26	0.0
27	8.037280120109113E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1244202.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.898661026833786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.93477928922741	49.436461137183144
2	10.039828485414372	11.826649094599423
3	2.6152536512938154	4.6210481492023145
4	1.1071914741751026	2.6084838131695904
5	0.588335178851022	1.7326077134653988
6	0.37664871653333926	1.3310463048773493
7	0.24099336625123371	0.9935930611982915
8	0.17062841610311913	0.803982819328253
9	0.12837256440671643	0.680487494052292
>10	0.6452083209297906	6.863746953096738
>50	0.06604537749111954	2.77080579669866
>100	0.07285720787158069	9.318590459231173
>500	0.01029055800851827	4.1961521286925025
>1k	0.0035673934429529997	2.8163450752047936
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2098	0.1686221369198892	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2006	0.16122783920938882	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1722	0.13840196366827895	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1679	0.134945933216632	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1622	0.13036468354816982	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1587	0.12755163550613166	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1507	0.12112181141004436	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1476	0.11863025457281053	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1458	0.11718354415119088	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1454	0.1168620529463865	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1429	0.11485273291635924	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1344	0.1080210448142665	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1333	0.10713694400105449	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1264	0.1015912207181792	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.607456024021823E-4
2	0.0	0.0	0.0	0.0	1.607456024021823E-4
3	0.0	0.0	0.0	0.0	1.607456024021823E-4
4	0.0	0.0	0.0	0.0	1.607456024021823E-4
5	0.0	0.0	0.0	0.0	1.607456024021823E-4
6	0.0	0.0	0.0	0.0	2.4111840360327343E-4
7	0.0	0.0	0.0	0.0	2.4111840360327343E-4
8	0.0	0.0	0.0	0.0	2.4111840360327343E-4
9	0.0	0.0	0.0	0.0	3.214912048043646E-4
10	0.0	0.0	0.0	0.0	3.214912048043646E-4
11	0.0	0.0	0.0	0.0	3.214912048043646E-4
12	0.0	0.0	0.0	0.0	4.8223680720654687E-4
13	0.0	0.0	0.0	0.0	4.8223680720654687E-4
14	0.0	0.0	0.0	0.0	4.8223680720654687E-4
15	0.0	0.0	0.0	0.0	5.62609608407638E-4
16	0.0	0.0	0.0	0.0	5.62609608407638E-4
17	0.0	0.0	0.0	0.0	5.62609608407638E-4
18	0.0	0.0	0.0	0.0	5.62609608407638E-4
19	0.0	0.0	0.0	0.0	5.62609608407638E-4
20	0.0	0.0	0.0	0.0	5.62609608407638E-4
21	0.0	0.0	0.0	0.0	7.233552108098203E-4
22	0.0	0.0	0.0	0.0	8.037280120109114E-4
23	0.0	0.0	0.0	8.037280120109114E-5	8.037280120109114E-4
24	0.0	8.037280120109114E-5	0.0	3.214912048043646E-4	8.037280120109114E-4
25	0.0	8.037280120109114E-5	0.0	4.8223680720654687E-4	8.037280120109114E-4
26	0.0	8.037280120109114E-5	0.0	6.429824096087292E-4	8.037280120109114E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATCG	40	0.0044822507	20.001104	9
TCTAGCG	415	0.0	18.891092	28
GGTATCA	600	0.0	18.403976	1
AAGACGG	445	0.0	18.338093	5
CTAGCGG	440	0.0	18.18136	29
CAAGACG	465	0.0	17.205252	4
GTATCAA	1250	0.0	17.155706	1
CGCTTCG	355	0.0	17.12633	32
TAGCGGC	470	0.0	17.02085	30
TTTTCGG	395	0.0	17.012232	29
GCGCAAG	475	0.0	16.845745	1
TAAACGC	365	0.0	16.657116	28
GTTTTCG	410	0.0	16.389832	28
CGACCCG	265	0.0	16.302788	5
AACCGGT	60	0.002441157	16.000885	7
TAGGATA	100	1.3138233E-6	16.000885	4
ACGCTTC	390	0.0	15.999598	31
AACGCTT	375	0.0	15.78627	30
GCGTAAC	245	0.0	15.673076	11
ATAACGA	215	0.0	15.627515	12
>>END_MODULE