FastQCFastQC Report
Thu 2 Feb 2017
SRR4063052_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063052_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328970
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28720.21610721084749843No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22730.17103471109204874No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22520.1694545399820914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18550.13958178137956462No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18260.13739964032295687No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17710.13326109693973529No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17530.13190666455977185No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17460.13137994085645274No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16950.12754238244655636No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16910.12724139747323115No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16440.12370482403665996No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15880.11949103441010707No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14740.11091296267033868No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14620.11001000775036306No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14270.10737638923376751No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13400.10082996606394425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA350.00206627122.85797511
CTAGCGG4050.020.9366729
TAGCGGC4100.020.68134530
TCTAGCG4250.019.95066528
CATCGTT2850.019.64688328
AGCGGCG4700.018.72197331
ATCGTTT3050.018.35925329
TATTCCC3000.017.5953455
AACGGAC854.983378E-616.94179315
GCGGCGC5150.016.77541732
AAGACGG5650.016.7035455
CGTTTAT3400.016.4693331
GCATCGT3400.016.4687127
CAAGACG5850.016.4059144
CGCAAGA5500.016.0054022
GTTATTC2600.015.9981753
TCGTTTA3550.015.77344330
AAACGCT3150.015.74482729
TTTCGTT4950.015.51338128
CCGACTT4850.015.505136523
Produced by FastQC (version 0.11.5)