##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063052_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1328970
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271440288343605	32.0	32.0	32.0	32.0	32.0
2	30.887247266680212	32.0	32.0	32.0	32.0	32.0
3	30.9395306139341	32.0	32.0	32.0	32.0	32.0
4	31.00590306778934	32.0	32.0	32.0	32.0	32.0
5	30.911469032408558	32.0	32.0	32.0	32.0	32.0
6	34.582019910155985	36.0	36.0	36.0	32.0	36.0
7	34.51826527310624	36.0	36.0	36.0	32.0	36.0
8	34.49712408857988	36.0	36.0	36.0	32.0	36.0
9	34.640223631835184	36.0	36.0	36.0	32.0	36.0
10	34.350401438708175	36.0	36.0	36.0	32.0	36.0
11	34.618747601525996	36.0	36.0	36.0	32.0	36.0
12	34.435288983197516	36.0	36.0	36.0	32.0	36.0
13	34.53234610262083	36.0	36.0	36.0	32.0	36.0
14	34.43154548259178	36.0	36.0	36.0	32.0	36.0
15	34.362346027374585	36.0	36.0	36.0	32.0	36.0
16	34.384953761183475	36.0	36.0	36.0	32.0	36.0
17	34.30813562382898	36.0	36.0	36.0	32.0	36.0
18	34.33257861351272	36.0	36.0	36.0	32.0	36.0
19	34.32441590103614	36.0	36.0	36.0	32.0	36.0
20	34.300566604212285	36.0	36.0	36.0	32.0	36.0
21	34.290171335696066	36.0	36.0	36.0	32.0	36.0
22	34.274064124848564	36.0	36.0	36.0	32.0	36.0
23	34.218443606702934	36.0	36.0	36.0	32.0	36.0
24	34.20299028570999	36.0	36.0	36.0	32.0	36.0
25	34.173191268425924	36.0	36.0	36.0	32.0	36.0
26	34.12850478189876	36.0	36.0	36.0	32.0	36.0
27	34.12786217898071	36.0	36.0	36.0	32.0	36.0
28	34.12213970217537	36.0	36.0	36.0	32.0	36.0
29	34.08441424561879	36.0	36.0	36.0	32.0	36.0
30	34.05564986418053	36.0	36.0	36.0	32.0	36.0
31	34.07697991677765	36.0	36.0	36.0	32.0	36.0
32	34.03536121959111	36.0	36.0	36.0	32.0	36.0
33	33.99599389000504	36.0	36.0	36.0	32.0	36.0
34	34.007413259893	36.0	36.0	36.0	32.0	36.0
35	33.969000805134804	36.0	36.0	36.0	32.0	36.0
36	33.9271006869982	36.0	36.0	36.0	32.0	36.0
37	33.92253775480259	36.0	36.0	36.0	32.0	36.0
38	33.33449513533037	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	5.0
9	7.0
10	13.0
11	10.0
12	2.0
13	6.0
14	113.0
15	282.0
16	397.0
17	480.0
18	565.0
19	746.0
20	1104.0
21	1545.0
22	2401.0
23	3608.0
24	5473.0
25	8234.0
26	12248.0
27	17628.0
28	24901.0
29	33501.0
30	45721.0
31	62016.0
32	85016.0
33	128611.0
34	290012.0
35	604321.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.824107356095475	17.1542528145038	11.148019205602864	25.873620623797862
2	17.215132120332385	19.190932834219605	36.88971940663489	26.704215638813118
3	19.281060753722745	22.895850438481297	28.10780626900694	29.71528253878902
4	12.563678858393267	14.709082618098416	35.813290953501074	36.91394757000724
5	14.9147004734515	36.134537880634845	33.433712275125586	15.517049370788072
6	35.06763905171389	35.15560217492396	16.30214768474137	13.474611088620778
7	30.97995513815597	30.091145843131027	20.305590273362284	18.62330874535072
8	28.711355177950914	32.474668199692694	19.071576162448586	19.74240045990781
9	27.373838006389928	14.070563129530777	18.479855767566665	40.075743096512625
10	16.15720852605908	26.002945157909075	31.003317629336003	26.83652868669585
11	38.297978007986835	21.004080705104908	21.55775631199974	19.140184974908518
12	24.92890902537453	23.584369692632652	28.102437222098064	23.384284059894757
13	29.342891889776578	18.806630383084787	25.280254546987035	26.570223180151597
14	23.598648465602096	19.65308610387851	24.60838613548455	32.13987929503484
15	25.382380611899297	26.922440070748504	22.02175140179882	25.673427915553383
16	26.3549294757129	25.596895922963984	23.22737319136415	24.820801409958964
17	24.327231073058535	25.854677697096683	24.700456390062982	25.1176348397818
18	25.583546103133344	24.35441014793427	25.80493204535176	24.257111703580627
19	25.803033712177363	24.818586058649963	25.126066039716537	24.252314189456133
20	25.882049283088044	24.043076422950215	24.710398271043438	25.364476022918307
21	27.300700058469896	23.92916187883262	24.113375910252852	24.656762152444635
22	25.989162277622384	23.98009748080522	24.94113450680537	25.089605734767023
23	24.376098219896075	23.760003913143272	25.589925161134964	26.27397270582569
24	24.95036838025572	24.864350273557147	25.012831222390297	25.172450123796835
25	24.947676190691215	24.387824601409598	25.105042728288723	25.55945647961046
26	24.748613148354558	25.262863985056395	25.583094822194745	24.405428044394302
27	25.6079280103668	24.480644282534325	24.58208476752219	25.329342939576687
28	24.60702135392798	24.427096619807976	25.712004539130056	25.25387748713399
29	24.499239556030158	24.8155330746093	25.695631266325464	24.989596103035076
30	24.477547248751623	25.047933516137867	25.823297645313048	24.65122158979746
31	25.204908163818168	24.86003335119288	24.45894612638537	25.476112358603586
32	24.896359546773844	24.666239738485167	24.441688558650824	25.99571215609016
33	24.530217276626036	24.285044538209092	25.13351679899824	26.051221386166628
34	25.38844701715887	24.5380665659756	25.32312575772285	24.750360659142682
35	26.15052323021753	24.290224381286382	25.21935782474704	24.339894563749045
36	24.58698956937943	25.134860548774064	24.945363416065867	25.332786465780643
37	25.97428887278463	24.787540420561978	24.652460301872082	24.58571040478131
38	24.653827370081324	24.51310831830198	25.369914266729577	25.46315004488712
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	99.5
2	135.0
3	135.0
4	338.5
5	542.0
6	542.0
7	675.5
8	809.0
9	844.5
10	880.0
11	880.0
12	1183.0
13	1486.0
14	2032.5
15	2579.0
16	2579.0
17	3777.0
18	4975.0
19	4975.0
20	6185.5
21	7396.0
22	8191.5
23	8987.0
24	8987.0
25	10635.5
26	12284.0
27	12284.0
28	15761.5
29	19239.0
30	23593.5
31	27948.0
32	27948.0
33	36041.5
34	44135.0
35	44135.0
36	49331.5
37	54528.0
38	62599.0
39	70670.0
40	70670.0
41	75325.5
42	79981.0
43	91844.5
44	103708.0
45	103708.0
46	105893.5
47	108079.0
48	108079.0
49	115240.0
50	122401.0
51	125061.5
52	127722.0
53	127722.0
54	121329.0
55	114936.0
56	114936.0
57	111354.0
58	107772.0
59	96709.0
60	85646.0
61	85646.0
62	81899.5
63	78153.0
64	64913.0
65	51673.0
66	51673.0
67	43487.0
68	35301.0
69	35301.0
70	28488.5
71	21676.0
72	17055.5
73	12435.0
74	12435.0
75	9445.5
76	6456.0
77	6456.0
78	6152.0
79	5848.0
80	4673.5
81	3499.0
82	3499.0
83	3498.5
84	3498.0
85	3498.0
86	2321.5
87	1145.0
88	994.0
89	843.0
90	843.0
91	596.5
92	350.0
93	303.5
94	257.0
95	257.0
96	239.5
97	222.0
98	222.0
99	467.0
100	712.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12408105525331647
2	0.056359436255144967
3	0.013469077556303
4	0.0027088647599268607
5	4.514774599878101E-4
6	3.0098497332520675E-4
7	7.524624333130169E-5
8	6.019699466504135E-4
9	3.0098497332520675E-4
10	6.772161899817152E-4
11	0.0024831260299329554
12	0.0029346034899207655
13	0.0133938313129717
14	0.008277086766443184
15	0.02407879786601654
16	0.01030873533638833
17	0.021219440619427075
18	0.005191990789859816
19	0.007750363063124073
20	0.005718714493178928
21	0.006094945709835437
22	0.006997900629811057
23	0.008653317983099694
24	0.013544323799634302
25	0.01693040474954288
26	0.018134344642843706
27	0.008427579253105789
28	0.006094945709835437
29	0.010609720309713537
30	0.0034613271932398774
31	0.0061701919531667385
32	0.007298885603136263
33	0.008352333009774487
34	0.011512675229689158
35	0.015425479882916846
36	0.014296786232947322
37	0.009706765389737918
38	0.006094945709835437
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1328970.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.11364021040938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.39631096581465	44.86046997024508
2	11.578704550551365	12.762891134034357
3	3.2049639369429737	5.2991168792403665
4	1.2925103882866302	2.8493981003298345
5	0.6691516649753303	1.8439692054823376
6	0.3889750484077542	1.2862698521262923
7	0.2533396763211468	0.9773730240249676
8	0.17864520212157156	0.7876629916035325
9	0.13978244444052124	0.6933527415563783
>10	0.719542572761569	7.2879347784768385
>50	0.07479085657328068	2.881775226932791
>100	0.08625205532306585	10.164509224094767
>500	0.011948915006302643	4.302363533014493
>1k	0.005081722473944802	4.002913338837967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2872	0.21610721084749843	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2273	0.17103471109204874	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2252	0.1694545399820914	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1855	0.13958178137956462	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1826	0.13739964032295687	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1771	0.13326109693973529	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1753	0.13190666455977185	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1746	0.13137994085645274	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1695	0.12754238244655636	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1691	0.12724139747323115	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1644	0.12370482403665996	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1588	0.11949103441010707	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1474	0.11091296267033868	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1462	0.11001000775036306	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1427	0.10737638923376751	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1340	0.10082996606394425	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.524624333130169E-5
2	0.0	0.0	0.0	0.0	7.524624333130169E-5
3	0.0	0.0	0.0	0.0	7.524624333130169E-5
4	0.0	0.0	0.0	0.0	7.524624333130169E-5
5	0.0	0.0	0.0	0.0	7.524624333130169E-5
6	0.0	0.0	0.0	0.0	7.524624333130169E-5
7	0.0	0.0	0.0	7.524624333130169E-5	7.524624333130169E-5
8	0.0	0.0	0.0	7.524624333130169E-5	7.524624333130169E-5
9	0.0	0.0	0.0	1.5049248666260337E-4	7.524624333130169E-5
10	0.0	0.0	0.0	1.5049248666260337E-4	7.524624333130169E-5
11	0.0	0.0	0.0	1.5049248666260337E-4	7.524624333130169E-5
12	0.0	0.0	0.0	1.5049248666260337E-4	2.2573872999390505E-4
13	0.0	0.0	0.0	1.5049248666260337E-4	3.0098497332520675E-4
14	0.0	0.0	0.0	1.5049248666260337E-4	3.0098497332520675E-4
15	0.0	0.0	0.0	1.5049248666260337E-4	5.267237033191118E-4
16	0.0	0.0	0.0	1.5049248666260337E-4	5.267237033191118E-4
17	0.0	0.0	0.0	1.5049248666260337E-4	5.267237033191118E-4
18	0.0	0.0	0.0	1.5049248666260337E-4	6.019699466504135E-4
19	0.0	0.0	0.0	2.2573872999390505E-4	6.019699466504135E-4
20	0.0	0.0	0.0	3.762312166565084E-4	8.277086766443185E-4
21	0.0	0.0	0.0	6.019699466504135E-4	8.277086766443185E-4
22	0.0	0.0	0.0	0.0010534474066382236	8.277086766443185E-4
23	0.0	0.0	0.0	0.0013544323799634303	9.78201163306922E-4
24	0.0	0.0	0.0	0.001956402326613844	9.78201163306922E-4
25	0.0	0.0	0.0	0.0022573872999390506	9.78201163306922E-4
26	0.0	0.0	0.0	0.0027841110032581625	9.78201163306922E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGA	35	0.002066271	22.857975	11
CTAGCGG	405	0.0	20.93667	29
TAGCGGC	410	0.0	20.681345	30
TCTAGCG	425	0.0	19.950665	28
CATCGTT	285	0.0	19.646883	28
AGCGGCG	470	0.0	18.721973	31
ATCGTTT	305	0.0	18.359253	29
TATTCCC	300	0.0	17.595345	5
AACGGAC	85	4.983378E-6	16.941793	15
GCGGCGC	515	0.0	16.775417	32
AAGACGG	565	0.0	16.703545	5
CGTTTAT	340	0.0	16.46933	31
GCATCGT	340	0.0	16.46871	27
CAAGACG	585	0.0	16.405914	4
CGCAAGA	550	0.0	16.005402	2
GTTATTC	260	0.0	15.998175	3
TCGTTTA	355	0.0	15.773443	30
AAACGCT	315	0.0	15.744827	29
TTTCGTT	495	0.0	15.513381	28
CCGACTT	485	0.0	15.5051365	23
>>END_MODULE