FastQCFastQC Report
Thu 2 Feb 2017
SRR4063052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328970
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30130.226716931157212No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23220.17472177701528252No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22640.17035749490206703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21220.15967252834902218No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19400.14597771206272528No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18620.14010850508288372No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18460.1389045651895829No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18320.1378511177829447No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17050.12829484487986936No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16230.12212465292670263No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15750.11851283324680016No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG15670.11791086330014974No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15410.1159544609735359No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15230.11460002859357246No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14680.11046148521035087No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13590.102259644687239No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13550.1019586597139138No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13420.10098045855060686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG5000.021.11977629
TCTAGCG5100.020.70566428
TAGCGGC5050.020.59384230
CATCGTT3350.019.58188228
ATCGTTT3450.019.01429229
TAAACGC3250.018.70749528
GCGGCGC5900.018.16929832
CGTTTAT3750.017.91981131
GAGTCGC450.00885152217.7775916
AGCGGCG5800.017.65498531
AAACGCT3450.017.62300129
AAGACGG4100.017.5614515
TCGTTTA3800.017.26297430
CGCTTCG3650.017.0957132
CAAGACG4350.016.9193614
AACGCTT3650.016.65735830
ACGCTTC3850.016.623231
GTAAACG3700.016.4322627
GGCATCG4000.016.39982626
GCATCGT4000.016.39982627
Produced by FastQC (version 0.11.5)