##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063052_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1328970
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206547175632256	32.0	32.0	32.0	32.0	32.0
2	31.273020459453562	32.0	32.0	32.0	32.0	32.0
3	31.3679481101906	32.0	32.0	32.0	32.0	32.0
4	31.474572789453486	32.0	32.0	32.0	32.0	32.0
5	31.389795104479408	32.0	32.0	32.0	32.0	32.0
6	34.91772726246642	36.0	36.0	36.0	36.0	36.0
7	34.95141124329368	36.0	36.0	36.0	36.0	36.0
8	34.87786556506166	36.0	36.0	36.0	32.0	36.0
9	35.00708292888478	36.0	36.0	36.0	36.0	36.0
10	34.844408075426834	36.0	36.0	36.0	32.0	36.0
11	35.021318765660624	36.0	36.0	36.0	36.0	36.0
12	34.91932323528748	36.0	36.0	36.0	32.0	36.0
13	34.97015433004507	36.0	36.0	36.0	36.0	36.0
14	34.9136744998006	36.0	36.0	36.0	32.0	36.0
15	34.88839401942858	36.0	36.0	36.0	32.0	36.0
16	34.891294762109	36.0	36.0	36.0	32.0	36.0
17	34.8566927771131	36.0	36.0	36.0	32.0	36.0
18	34.8578199658382	36.0	36.0	36.0	32.0	36.0
19	34.85950999646343	36.0	36.0	36.0	32.0	36.0
20	34.84692731965357	36.0	36.0	36.0	32.0	36.0
21	34.830419798791546	36.0	36.0	36.0	32.0	36.0
22	34.82185451891314	36.0	36.0	36.0	32.0	36.0
23	34.76589539267252	36.0	36.0	36.0	32.0	36.0
24	34.735091838039985	36.0	36.0	36.0	32.0	36.0
25	34.717191509213905	36.0	36.0	36.0	32.0	36.0
26	34.64841719527153	36.0	36.0	36.0	32.0	36.0
27	34.63620472997886	36.0	36.0	36.0	32.0	36.0
28	34.61383552676133	36.0	36.0	36.0	32.0	36.0
29	34.574508830146655	36.0	36.0	36.0	32.0	36.0
30	34.54606951247959	36.0	36.0	36.0	32.0	36.0
31	34.550630939750334	36.0	36.0	36.0	32.0	36.0
32	34.53383372085148	36.0	36.0	36.0	32.0	36.0
33	34.4951752108776	36.0	36.0	36.0	32.0	36.0
34	34.48604784156151	36.0	36.0	36.0	32.0	36.0
35	34.457145759497955	36.0	36.0	36.0	32.0	36.0
36	34.42283422500132	36.0	36.0	36.0	32.0	36.0
37	34.42536325123968	36.0	36.0	36.0	32.0	36.0
38	33.987835692303065	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	26.0
22	108.0
23	285.0
24	869.0
25	2136.0
26	4465.0
27	8905.0
28	14964.0
29	23954.0
30	36348.0
31	53116.0
32	76106.0
33	124202.0
34	306537.0
35	676941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.14911697154502	18.379243187270664	11.851349602208249	26.620290238976068
2	16.21497566910891	20.16081639225595	36.14960168371121	27.474606254923927
3	18.21147204225829	23.680218515090633	28.431341565272355	29.676967877378722
4	12.047769397005798	15.592023284232578	35.80417286195002	36.5560344568116
5	14.33960134540283	36.71715689594197	33.0789257846302	15.864315974024997
6	35.182487463956996	35.675953070352335	15.896771028346807	13.244788437343862
7	30.859902901809228	30.29021102151751	20.179402903314163	18.6704831733591
8	28.691610374893056	32.66952057242658	18.834047311426634	19.804821741253736
9	27.24548014393708	13.905564995176512	18.324135346606326	40.52481951428008
10	15.842736704601206	26.359864434939904	31.029093514006483	26.768305346452404
11	38.47536716540108	20.913057972137796	21.52055426417548	19.091020598285645
12	24.77830492149906	23.71093294405797	27.977110006659316	23.533652127783654
13	29.289666334829985	18.81077558735469	25.399803004890266	26.499755072925062
14	23.474420039579524	19.65040595348277	24.555407571277005	32.3197664356607
15	25.29169206227379	27.1518544436669	21.792967486098256	25.76348600796105
16	26.355994759847672	25.71519726946227	23.010393771412275	24.918414199277787
17	24.290164563534166	25.953031294912606	24.60424238319902	25.152561758354214
18	25.62232405547153	24.435239320676917	25.67304002347683	24.269396600374726
19	25.75280104140801	24.864293400151997	25.13909268079791	24.243812877642085
20	25.917439821816895	24.030188792824518	24.625010346358458	25.42736103900013
21	27.28259822659387	24.018035046743037	24.12992637896473	24.569440347698364
22	25.87522667930803	24.044335086570804	24.945258357976478	25.13517987614468
23	24.329780702018407	23.692895539997956	25.650053274420454	26.327270483563186
24	24.953234459769597	24.874602135488384	24.971519296899103	25.20064410784292
25	24.965367890447407	24.378973474926806	25.11187243645262	25.54378619817317
26	24.78887737618767	25.23817370935471	25.451045812273744	24.521903102183877
27	25.68067854154502	24.431363283389818	24.513080826626375	25.37487734843879
28	24.59513758775593	24.465638802982763	25.697043575099514	25.242180034161795
29	24.646004048247892	24.66383740791741	25.610660887755177	25.079497656079518
30	24.616206535888697	24.894692882457843	25.760250419497805	24.728850162155656
31	25.274159687577598	24.724937357502426	24.53779995033748	25.463103004582493
32	25.00026336185166	24.535392070550877	24.512592458821494	25.95175210877597
33	24.48828792222548	24.27210546513465	25.213435969209236	26.02617064343063
34	25.4604693860659	24.413643648840832	25.328186490289472	24.797700474803797
35	26.24664213639134	24.202653182539862	25.124795894564965	24.425908786503832
36	24.65706524601759	25.051656546046942	24.901013566897674	25.390264641037795
37	26.004123494134557	24.713951405976058	24.595363326485923	24.68656177340346
38	24.78026199256717	24.233371884521006	25.440473028340016	25.54589309457181
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	43.0
2	61.0
3	61.0
4	251.5
5	442.0
6	442.0
7	596.5
8	751.0
9	804.0
10	857.0
11	857.0
12	1159.5
13	1462.0
14	2058.0
15	2654.0
16	2654.0
17	3898.0
18	5142.0
19	5142.0
20	6215.0
21	7288.0
22	8134.0
23	8980.0
24	8980.0
25	10510.0
26	12040.0
27	12040.0
28	15680.0
29	19320.0
30	23483.0
31	27646.0
32	27646.0
33	36069.0
34	44492.0
35	44492.0
36	49580.5
37	54669.0
38	62550.0
39	70431.0
40	70431.0
41	75489.0
42	80547.0
43	92269.0
44	103991.0
45	103991.0
46	105987.0
47	107983.0
48	107983.0
49	115668.5
50	123354.0
51	125609.0
52	127864.0
53	127864.0
54	121288.5
55	114713.0
56	114713.0
57	111623.0
58	108533.0
59	97294.0
60	86055.0
61	86055.0
62	82497.0
63	78939.0
64	65299.0
65	51659.0
66	51659.0
67	43506.0
68	35353.0
69	35353.0
70	28404.5
71	21456.0
72	16807.5
73	12159.0
74	12159.0
75	9172.5
76	6186.0
77	6186.0
78	6094.5
79	6003.0
80	4693.0
81	3383.0
82	3383.0
83	3180.0
84	2977.0
85	2977.0
86	1932.0
87	887.0
88	697.0
89	507.0
90	507.0
91	302.0
92	97.0
93	63.5
94	30.0
95	30.0
96	25.5
97	21.0
98	21.0
99	17.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010082996606394426
2	7.524624333130169E-5
3	0.0
4	1.5049248666260337E-4
5	0.0
6	1.5049248666260337E-4
7	4.514774599878101E-4
8	0.002332633543270352
9	0.0042137896265528945
10	0.0010534474066382236
11	0.005492975763185023
12	3.762312166565084E-4
13	2.2573872999390505E-4
14	0.0
15	0.0
16	7.524624333130169E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5049248666260337E-4
22	0.0
23	1.5049248666260337E-4
24	0.0
25	7.524624333130169E-5
26	2.2573872999390505E-4
27	1.5049248666260337E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	7.524624333130169E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1328970.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.30815112789965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.15785434358503	42.73067013435998
2	12.436789174275251	13.259644736961828
3	3.3041331397929232	5.284116862883483
4	1.3510917294083462	2.8809680839582166
5	0.7135588987274568	1.9019252806010454
6	0.4262402969976124	1.363324930148973
7	0.28128686028985983	1.0496417721036968
8	0.20955954190921522	0.8936985384311891
9	0.14181944842632807	0.680411933062746
>10	0.7894055814684753	7.6855136183734905
>50	0.07881408657169682	2.958418966636695
>100	0.09125314325650108	10.477463619739229
>500	0.01279248418918776	4.617667974944574
>1k	0.005401271102101498	4.216533547794881
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3013	0.226716931157212	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2322	0.17472177701528252	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2264	0.17035749490206703	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2122	0.15967252834902218	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1940	0.14597771206272528	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1862	0.14010850508288372	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1846	0.1389045651895829	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1832	0.1378511177829447	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1705	0.12829484487986936	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1623	0.12212465292670263	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1575	0.11851283324680016	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1567	0.11791086330014974	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1541	0.1159544609735359	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1523	0.11460002859357246	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1468	0.11046148521035087	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1359	0.102259644687239	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1355	0.1019586597139138	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1342	0.10098045855060686	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.0098497332520675E-4
2	0.0	0.0	0.0	0.0	3.0098497332520675E-4
3	0.0	0.0	0.0	0.0	3.0098497332520675E-4
4	0.0	0.0	0.0	0.0	3.762312166565084E-4
5	0.0	0.0	0.0	0.0	3.762312166565084E-4
6	0.0	0.0	0.0	0.0	3.762312166565084E-4
7	0.0	0.0	0.0	0.0	3.762312166565084E-4
8	0.0	0.0	0.0	0.0	3.762312166565084E-4
9	0.0	0.0	0.0	7.524624333130169E-5	4.514774599878101E-4
10	0.0	0.0	0.0	7.524624333130169E-5	4.514774599878101E-4
11	0.0	0.0	0.0	7.524624333130169E-5	4.514774599878101E-4
12	0.0	0.0	0.0	7.524624333130169E-5	5.267237033191118E-4
13	0.0	0.0	0.0	7.524624333130169E-5	5.267237033191118E-4
14	0.0	0.0	0.0	7.524624333130169E-5	5.267237033191118E-4
15	0.0	0.0	0.0	7.524624333130169E-5	5.267237033191118E-4
16	0.0	0.0	0.0	7.524624333130169E-5	5.267237033191118E-4
17	0.0	0.0	0.0	7.524624333130169E-5	5.267237033191118E-4
18	0.0	0.0	0.0	7.524624333130169E-5	5.267237033191118E-4
19	0.0	0.0	0.0	7.524624333130169E-5	5.267237033191118E-4
20	0.0	0.0	0.0	1.5049248666260337E-4	6.019699466504135E-4
21	0.0	0.0	0.0	2.2573872999390505E-4	6.772161899817152E-4
22	0.0	0.0	0.0	7.524624333130168E-4	6.772161899817152E-4
23	0.0	0.0	0.0	0.0010534474066382236	6.772161899817152E-4
24	0.0	0.0	0.0	0.001655417353288637	6.772161899817152E-4
25	0.0	0.0	0.0	0.001956402326613844	6.772161899817152E-4
26	0.0	0.0	0.0	0.0024831260299329554	7.524624333130168E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	500	0.0	21.119776	29
TCTAGCG	510	0.0	20.705664	28
TAGCGGC	505	0.0	20.593842	30
CATCGTT	335	0.0	19.581882	28
ATCGTTT	345	0.0	19.014292	29
TAAACGC	325	0.0	18.707495	28
GCGGCGC	590	0.0	18.169298	32
CGTTTAT	375	0.0	17.919811	31
GAGTCGC	45	0.008851522	17.77759	16
AGCGGCG	580	0.0	17.654985	31
AAACGCT	345	0.0	17.623001	29
AAGACGG	410	0.0	17.561451	5
TCGTTTA	380	0.0	17.262974	30
CGCTTCG	365	0.0	17.09571	32
CAAGACG	435	0.0	16.919361	4
AACGCTT	365	0.0	16.657358	30
ACGCTTC	385	0.0	16.6232	31
GTAAACG	370	0.0	16.43226	27
GGCATCG	400	0.0	16.399826	26
GCATCGT	400	0.0	16.399826	27
>>END_MODULE