FastQCFastQC Report
Thu 2 Feb 2017
SRR4063051_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063051_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953458
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43170.4527729590605984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27580.28926287261735706No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16880.17703978570634468No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16260.17053713954888416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16130.1691736814836102No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15260.16004900058523816No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15060.15795137279250895No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14560.15270730331068594No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14460.15165848941432136No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14340.15039991273868383No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14110.14798764077704524No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13770.1444216735294056No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13210.13854831570976384No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13140.13781414598230862No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13120.1376043832030357No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12650.13267495789012207No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT12630.13246519511084914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12610.13225543233157622No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12540.131521262604121No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT12380.12984316036993765No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11720.12292098865393127No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11320.11872573306847287No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC11250.11799156334101764No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT11150.11694274944465304No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11110.1165232238861072No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT11050.11589393554828843No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG11030.11568417276901552No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT10950.11484512165192383No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT10400.10907664522191853No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA10300.10802783132555394No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10270.10771318715664456No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT10020.10509115241573305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAG908.6401997E-1021.3597011
ATCGTTT2500.017.91955829
GTATTAG2350.017.7240071
GTATTAT652.0801998E-417.2520661
CATCGTT2600.017.23034728
TGTTAGG753.22036E-517.0743082
TAACCCG957.690978E-716.84169228
CGGCATC2900.016.55131725
GTCTAGA908.477724E-616.0197751
GTGCTAA805.5601828E-516.0197751
GTATAAT703.6609746E-416.0197751
GTTATAT703.6609746E-416.0197751
GTTCTAA1203.0609954E-816.0197731
CGCAATC600.002441041616.00044412
AACCCGT908.579724E-615.99960729
CGTTTAT3000.015.99876831
CCCGTTG1001.3148492E-615.99876731
ACCGTCG2950.015.7267778
AGGCCCG4300.015.62834310
TCGTTTA3000.015.46547630
Produced by FastQC (version 0.11.5)