##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063051_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	953458
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17467261274225	32.0	32.0	32.0	32.0	32.0
2	30.754977146345198	32.0	32.0	32.0	32.0	32.0
3	30.814397697643734	32.0	32.0	32.0	32.0	32.0
4	30.88140956392416	32.0	32.0	32.0	32.0	32.0
5	30.78693660339522	32.0	32.0	32.0	32.0	32.0
6	34.44038436931674	36.0	36.0	36.0	32.0	36.0
7	34.33572427941241	36.0	36.0	36.0	32.0	36.0
8	34.32642969066283	36.0	36.0	36.0	32.0	36.0
9	34.471401991487824	36.0	36.0	36.0	32.0	36.0
10	34.164651195962485	36.0	36.0	36.0	32.0	36.0
11	34.48357242794124	36.0	36.0	36.0	32.0	36.0
12	34.27995360047323	36.0	36.0	36.0	32.0	36.0
13	34.37899414552083	36.0	36.0	36.0	32.0	36.0
14	34.26674169182072	36.0	36.0	36.0	32.0	36.0
15	34.205061995389414	36.0	36.0	36.0	32.0	36.0
16	34.22280268244642	36.0	36.0	36.0	32.0	36.0
17	34.12631704805036	36.0	36.0	36.0	32.0	36.0
18	34.167992717036306	36.0	36.0	36.0	32.0	36.0
19	34.15195635256089	36.0	36.0	36.0	32.0	36.0
20	34.12618175105773	36.0	36.0	36.0	32.0	36.0
21	34.10507961546287	36.0	36.0	36.0	32.0	36.0
22	34.08223225354447	36.0	36.0	36.0	32.0	36.0
23	34.030257232096226	36.0	36.0	36.0	32.0	36.0
24	34.01043989352441	36.0	36.0	36.0	32.0	36.0
25	33.99273171969819	36.0	36.0	36.0	32.0	36.0
26	33.940456737475586	36.0	36.0	36.0	32.0	36.0
27	33.945457482133456	36.0	36.0	36.0	32.0	36.0
28	33.92255663070633	36.0	36.0	36.0	32.0	36.0
29	33.89168479366684	36.0	36.0	36.0	32.0	36.0
30	33.851041157554924	36.0	36.0	36.0	32.0	36.0
31	33.88327959910138	36.0	36.0	36.0	32.0	36.0
32	33.8165718888509	36.0	36.0	36.0	32.0	36.0
33	33.77388516326886	36.0	36.0	36.0	32.0	36.0
34	33.78007001881572	36.0	36.0	36.0	32.0	36.0
35	33.7239060346654	36.0	36.0	36.0	27.0	36.0
36	33.67571408494134	36.0	36.0	36.0	27.0	36.0
37	33.67126921164855	36.0	36.0	36.0	27.0	36.0
38	33.052673531503224	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	2.0
10	5.0
11	4.0
12	3.0
13	9.0
14	199.0
15	347.0
16	426.0
17	566.0
18	643.0
19	845.0
20	1118.0
21	1486.0
22	2236.0
23	3239.0
24	4764.0
25	6931.0
26	10018.0
27	14199.0
28	19833.0
29	26391.0
30	35744.0
31	48132.0
32	65121.0
33	98114.0
34	212304.0
35	400775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.418861362433525	17.519972781572115	11.629157320833018	25.432008535161337
2	16.872815343463948	20.41485781565303	36.75763652562088	25.954690315262134
3	18.23163389039705	25.1198952725808	28.690005160846376	27.95846567617577
4	12.554605648885547	15.540990553026084	35.81711027692734	36.08729352116104
5	14.835220157448603	36.41329314261622	32.80095316606779	15.950533533867391
6	33.510478743599585	35.807495694600895	16.90275566519203	13.779269896607493
7	29.764184683541384	30.85316815213675	21.428946004962988	17.953701159358882
8	28.76216367019279	31.23244540376358	19.970863827725303	20.034527098318325
9	28.248153823402628	13.856838850624623	18.77693488012569	39.118072445847055
10	16.669078948748446	25.70307534500878	30.35930417198942	27.268541534253355
11	37.3986698732908	21.275105066585	22.214122920824842	19.11210213929936
12	25.564087208149832	23.818833427902874	27.77344843897328	22.843630924974015
13	29.608401760576676	19.027004460183527	25.37353512551871	25.991058653721083
14	24.659682349214474	19.77545013814096	24.733735134375387	30.831132378269178
15	25.497185839501057	26.275394323421263	22.843429866294592	25.383989970783087
16	26.154548124527988	25.299257363430392	23.312075186708064	25.23411932533356
17	24.087012349408965	25.34471761689557	25.0612644821263	25.50700555156917
18	25.682340231379996	23.61428975991441	26.398821073829726	24.304548934875868
19	25.988797751159034	24.595334494115672	25.525708531750197	23.8901592229751
20	26.196599574160118	23.191806253343263	25.055117945060363	25.556476227436253
21	28.11810362911685	23.53303964757709	24.037654709460877	24.311202013845186
22	26.517019631864517	23.322869019161967	24.73224746407328	25.427863884900237
23	24.30239644377687	23.221599985734827	25.744332479171277	26.731671091317022
24	24.974903521479124	24.91846922495151	25.027456648914686	25.079170604654678
25	25.2021151943841	23.74317235099807	25.17924709402041	25.87546536059742
26	24.91694387010619	24.844351711108384	25.764866444064698	24.473837974720723
27	25.835452119625412	24.3163243043668	24.537433289698924	25.310790286308865
28	24.523126752940534	23.967010629348916	26.048827457158502	25.461035160552047
29	24.421911204302273	24.55239747544729	25.85967271441863	25.166018605831812
30	24.562029134777454	24.628525818858417	26.113583468546754	24.695861577817375
31	25.31046519433443	24.517415419211922	24.31624236423441	25.855877022219236
32	24.722336141875246	24.553467009159842	24.451411315910093	26.272785533054822
33	24.23696990675184	24.062431165234905	25.221477495620796	26.47912143239246
34	25.45935915765536	24.15751374904415	25.457366162508826	24.92576093079167
35	26.552628073130997	23.905096281035515	25.308112950447025	24.23416269538646
36	24.83145725617553	25.10544738959362	24.582012946348495	25.481082407882354
37	26.03504429048685	24.903053919537637	24.671243437717976	24.390658352257528
38	24.760881855340735	24.534114258563307	25.332835465529126	25.372168420566837
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	806.0
1	602.5
2	399.0
3	399.0
4	821.5
5	1244.0
6	1244.0
7	1449.5
8	1655.0
9	1520.5
10	1386.0
11	1386.0
12	1792.0
13	2198.0
14	2752.5
15	3307.0
16	3307.0
17	4680.0
18	6053.0
19	6053.0
20	6938.0
21	7823.0
22	7529.5
23	7236.0
24	7236.0
25	7755.5
26	8275.0
27	8275.0
28	10261.0
29	12247.0
30	15196.5
31	18146.0
32	18146.0
33	23889.0
34	29632.0
35	29632.0
36	32809.5
37	35987.0
38	41829.0
39	47671.0
40	47671.0
41	51149.5
42	54628.0
43	64275.0
44	73922.0
45	73922.0
46	75927.5
47	77933.0
48	77933.0
49	83281.5
50	88630.0
51	88416.0
52	88202.0
53	88202.0
54	83912.0
55	79622.0
56	79622.0
57	78643.0
58	77664.0
59	69631.5
60	61599.0
61	61599.0
62	60457.5
63	59316.0
64	48760.0
65	38204.0
66	38204.0
67	32031.5
68	25859.0
69	25859.0
70	21172.5
71	16486.0
72	12821.5
73	9157.0
74	9157.0
75	6936.0
76	4715.0
77	4715.0
78	4695.5
79	4676.0
80	3703.5
81	2731.0
82	2731.0
83	2907.0
84	3083.0
85	3083.0
86	1998.0
87	913.0
88	823.0
89	733.0
90	733.0
91	535.5
92	338.0
93	300.0
94	262.0
95	262.0
96	233.0
97	204.0
98	204.0
99	360.0
100	516.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12187217475756666
2	0.052965101766412365
3	0.013215055094193975
4	0.0032513230787302636
5	4.195255585458405E-4
6	4.195255585458405E-4
7	0.0
8	4.195255585458405E-4
9	1.0488138963646013E-4
10	4.195255585458405E-4
11	0.001573220844546902
12	0.001573220844546902
13	0.014054106211285658
14	0.00881003672946265
15	0.025486177681659814
16	0.010278376184373092
17	0.022444617382202468
18	0.005034306702550086
19	0.00818074839164389
20	0.005034306702550086
21	0.006083120598914688
22	0.0061880019885511475
23	0.009124680898372031
24	0.014368750380195036
25	0.01730542929001592
26	0.019612819862018044
27	0.008600273950189731
28	0.006292883378187608
29	0.010593020353282473
30	0.0028317975201844234
31	0.0052440694818230065
32	0.005978239209278227
33	0.00922956228800849
34	0.012480885366738755
35	0.01625661539365132
36	0.014578513159467957
37	0.009753969236190792
38	0.0061880019885511475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	953458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.839372554117475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.95487309776796	39.84900707836842
2	12.09175146969304	12.05290612659658
3	3.519209321368795	5.26185553390867
4	1.462015120719725	2.9146366512521364
5	0.7710770824006282	1.9214998988853427
6	0.45809047522956203	1.369856511507532
7	0.30669342884334716	1.0699785642016308
8	0.21623839741360767	0.8621748839361685
9	0.1632764204921664	0.732383491519064
>10	0.8088107628569831	7.56042834300425
>50	0.10337264251575425	3.6355870607557748
>100	0.12281845701290962	13.132122718508166
>500	0.015008795938576425	4.849861645799198
>1k	0.006764527746964024	4.787701491757065
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4317	0.4527729590605984	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2758	0.28926287261735706	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1688	0.17703978570634468	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1626	0.17053713954888416	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1613	0.1691736814836102	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1526	0.16004900058523816	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1506	0.15795137279250895	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1456	0.15270730331068594	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1446	0.15165848941432136	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1434	0.15039991273868383	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1411	0.14798764077704524	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1377	0.1444216735294056	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1321	0.13854831570976384	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1314	0.13781414598230862	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1312	0.1376043832030357	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1265	0.13267495789012207	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1263	0.13246519511084914	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1261	0.13225543233157622	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1254	0.131521262604121	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1238	0.12984316036993765	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1172	0.12292098865393127	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1132	0.11872573306847287	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1125	0.11799156334101764	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1115	0.11694274944465304	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1111	0.1165232238861072	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1105	0.11589393554828843	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1103	0.11568417276901552	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1095	0.11484512165192383	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1040	0.10907664522191853	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1030	0.10802783132555394	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1027	0.10771318715664456	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1002	0.10509115241573305	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.0488138963646013E-4
10	0.0	0.0	0.0	0.0	1.0488138963646013E-4
11	0.0	0.0	0.0	0.0	1.0488138963646013E-4
12	0.0	0.0	0.0	0.0	1.0488138963646013E-4
13	0.0	0.0	0.0	0.0	1.0488138963646013E-4
14	0.0	0.0	0.0	0.0	1.0488138963646013E-4
15	0.0	0.0	0.0	0.0	1.0488138963646013E-4
16	0.0	0.0	0.0	0.0	1.0488138963646013E-4
17	0.0	0.0	0.0	0.0	1.0488138963646013E-4
18	0.0	0.0	0.0	1.0488138963646013E-4	1.0488138963646013E-4
19	0.0	0.0	0.0	1.0488138963646013E-4	1.0488138963646013E-4
20	0.0	0.0	0.0	1.0488138963646013E-4	1.0488138963646013E-4
21	0.0	0.0	0.0	4.195255585458405E-4	1.0488138963646013E-4
22	0.0	0.0	0.0	6.292883378187608E-4	1.0488138963646013E-4
23	0.0	0.0	0.0	0.001678102234183362	1.0488138963646013E-4
24	0.0	0.0	0.0	0.0023073905720021226	1.0488138963646013E-4
25	0.0	0.0	0.0	0.003565967247639644	1.0488138963646013E-4
26	0.0	0.0	0.0	0.004824543923277166	1.0488138963646013E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAG	90	8.6401997E-10	21.359701	1
ATCGTTT	250	0.0	17.919558	29
GTATTAG	235	0.0	17.724007	1
GTATTAT	65	2.0801998E-4	17.252066	1
CATCGTT	260	0.0	17.230347	28
TGTTAGG	75	3.22036E-5	17.074308	2
TAACCCG	95	7.690978E-7	16.841692	28
CGGCATC	290	0.0	16.551317	25
GTCTAGA	90	8.477724E-6	16.019775	1
GTGCTAA	80	5.5601828E-5	16.019775	1
GTATAAT	70	3.6609746E-4	16.019775	1
GTTATAT	70	3.6609746E-4	16.019775	1
GTTCTAA	120	3.0609954E-8	16.019773	1
CGCAATC	60	0.0024410416	16.000444	12
AACCCGT	90	8.579724E-6	15.999607	29
CGTTTAT	300	0.0	15.998768	31
CCCGTTG	100	1.3148492E-6	15.998767	31
ACCGTCG	295	0.0	15.726777	8
AGGCCCG	430	0.0	15.628343	10
TCGTTTA	300	0.0	15.465476	30
>>END_MODULE