FastQCFastQC Report
Thu 2 Feb 2017
SRR4063051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences953458
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50100.5254557620786652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34920.36624581261051875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20840.21857281600238287No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17900.18773768744926364No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17020.17850812516125514No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16330.17127130927633938No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15320.1606782889230569No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15210.15952459363705584No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14720.1543854055448693No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14690.15407076137595993No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14640.15354635442777761No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14490.15197313358323072No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13850.14526072464649728No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13660.14326797824340454No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13440.1409605876714024No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13310.1395971296061284No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13270.1391776040475826No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12950.13582139957921585No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12780.13403841595539603No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12730.13351400900721372No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12630.13246519511084914No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG12590.13204566955230332No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT12210.1280601767461178No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT12110.12701136284975323No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT11950.12533326061556985No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT11730.12302587004356773No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC11710.1228161072642948No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA10840.11369142636592279No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT10780.11306213802810403No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG10250.10750342437737163No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10110.10603508492246119No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTT350.00206439422.860749
TATGGGT555.8343096E-520.3625683
TAAGACT659.435322E-619.6912754
CGATAAC1450.018.76157210
ACGAACG1450.018.75763715
CGAACGA1401.8189894E-1218.28475616
CCGATAA1550.017.5511479
TAACGAA1551.0913936E-1116.51526313
ATAACGA1651.8189894E-1216.48484812
ACCCCGC600.002438933116.0025186
ACTATAC805.621767E-515.9991613
TATTAGA1304.800313E-915.9991612
CGGTCCA4050.015.80495610
ATACCGT4050.015.8049566
CGCCGGT3950.015.7999557
TACCGTC3750.015.7891517
CGTCGTA3600.015.55800210
TCAGATA3600.015.554742
GATAACG1753.6379788E-1215.54530311
CCGGTCC4250.015.4377239
Produced by FastQC (version 0.11.5)