##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063051_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	953458
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15294538406516	32.0	32.0	32.0	32.0	32.0
2	31.290694503585893	32.0	32.0	32.0	32.0	32.0
3	31.36775715343518	32.0	32.0	32.0	32.0	32.0
4	31.47001336188904	32.0	32.0	32.0	32.0	32.0
5	31.384315827230985	32.0	32.0	32.0	32.0	32.0
6	34.91554740743693	36.0	36.0	36.0	36.0	36.0
7	34.933884869600966	36.0	36.0	36.0	36.0	36.0
8	34.886107201365974	36.0	36.0	36.0	32.0	36.0
9	34.98640422546143	36.0	36.0	36.0	36.0	36.0
10	34.82509769701445	36.0	36.0	36.0	32.0	36.0
11	35.01388629598787	36.0	36.0	36.0	36.0	36.0
12	34.90318189159879	36.0	36.0	36.0	32.0	36.0
13	34.956119724203894	36.0	36.0	36.0	36.0	36.0
14	34.895208808358625	36.0	36.0	36.0	32.0	36.0
15	34.867404751966	36.0	36.0	36.0	32.0	36.0
16	34.86883952937623	36.0	36.0	36.0	32.0	36.0
17	34.836494108812346	36.0	36.0	36.0	32.0	36.0
18	34.82423976724722	36.0	36.0	36.0	32.0	36.0
19	34.82501588953053	36.0	36.0	36.0	32.0	36.0
20	34.812834964938155	36.0	36.0	36.0	32.0	36.0
21	34.79666854753959	36.0	36.0	36.0	32.0	36.0
22	34.7680464163078	36.0	36.0	36.0	32.0	36.0
23	34.72080783841554	36.0	36.0	36.0	32.0	36.0
24	34.68257437663746	36.0	36.0	36.0	32.0	36.0
25	34.663221662621744	36.0	36.0	36.0	32.0	36.0
26	34.59624965126938	36.0	36.0	36.0	32.0	36.0
27	34.58375932657757	36.0	36.0	36.0	32.0	36.0
28	34.54849925219569	36.0	36.0	36.0	32.0	36.0
29	34.50966272242721	36.0	36.0	36.0	32.0	36.0
30	34.47896603730841	36.0	36.0	36.0	32.0	36.0
31	34.48161534121063	36.0	36.0	36.0	32.0	36.0
32	34.432529802046865	36.0	36.0	36.0	32.0	36.0
33	34.38963121605776	36.0	36.0	36.0	32.0	36.0
34	34.37265196788952	36.0	36.0	36.0	32.0	36.0
35	34.322619349777334	36.0	36.0	36.0	32.0	36.0
36	34.296709451281544	36.0	36.0	36.0	32.0	36.0
37	34.277725919757344	36.0	36.0	36.0	32.0	36.0
38	33.82362830874564	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	25.0
22	70.0
23	270.0
24	693.0
25	1679.0
26	3515.0
27	6821.0
28	11885.0
29	18386.0
30	27590.0
31	39510.0
32	56797.0
33	90395.0
34	218149.0
35	477662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98091496960765	18.661058461344815	12.31102463379713	26.047001935250403
2	15.772692661868692	21.41856274738898	36.082134713852106	26.726609876890222
3	17.25152025574278	25.85347230816669	29.09567070599859	27.799336730091937
4	12.02760061544464	16.354172238496332	35.903034955955015	35.71519219010401
5	14.2098699784049	36.90087754350746	32.72523039843433	16.164022079653304
6	33.432131705991985	36.19953495543061	16.626339730055996	13.741993608521414
7	29.51119874561463	31.066489766166207	21.35622551667357	18.06608597154559
8	28.452191788724047	31.470509573179367	19.898892961838385	20.1784056762582
9	28.203883036912032	13.63919075253637	18.566353656119126	39.59057255443248
10	16.500725261655315	25.872700060517133	30.452074521028393	27.17450015679916
11	37.428584914071145	21.189840623869184	22.21395944011202	19.16761502194765
12	25.315929959987628	24.004698701039903	27.868226607443457	22.81114473152902
13	29.560326265369074	18.998969014858563	25.459564511037204	25.98114020873516
14	24.574443761550064	19.69588592261012	24.717816621183104	31.01185369465671
15	25.496770702013094	26.323655577906948	22.7666032483864	25.412970471693562
16	26.027260770794307	25.37185696695607	23.303176437766528	25.297705824483092
17	24.103945847640905	25.504636806235826	24.984844639197533	25.40657270692574
18	25.68251564305926	23.527098204640374	26.46010626582398	24.330279886476383
19	25.973037092352257	24.427504934669383	25.77334292648444	23.82611504649392
20	26.23219900614395	22.96975850011222	25.239916178793404	25.558126314950425
21	27.98427197031434	23.5461064316482	24.219970066820004	24.249651531217452
22	26.439339750675963	23.26877534196577	24.92474760293584	25.367137304422428
23	24.24417226558485	23.008879258446623	25.909688733011837	26.837259742956693
24	24.915727803427103	24.67911538840725	25.235720923207943	25.169435884957704
25	25.14227160504186	23.65285099081449	25.45859387618542	25.74628352795823
26	24.961665852087876	24.577380440459883	25.93360168984895	24.52735201760329
27	25.82987399549849	23.992981337405528	24.678905625627976	25.498239041468
28	24.564060503976055	23.690083884135433	26.368439931281713	25.377415680606802
29	24.68918400181235	24.013747852553546	26.111690289451662	25.185377856182445
30	24.535952291553482	24.286544346997978	26.347044127795876	24.830459233652665
31	25.235720923207943	24.245430842260486	24.686457085681802	25.832391148849766
32	24.859196734413054	24.163728239733683	24.694952478242353	26.28212254761091
33	24.420268118784467	23.64362142852648	25.498343922857643	26.437766529831414
34	25.30274013118564	23.802202089656806	25.869833804949984	25.025223974207567
35	26.52355950655404	23.66082197642686	25.516803047433655	24.298815469585445
36	24.95432415481332	24.707223600829824	24.89034650713508	25.448105737221777
37	26.09826547157819	24.58608559579971	24.862343176102147	24.453305756519953
38	24.796608551827717	24.009158252548357	25.725543994118244	25.46868920150568
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	398.0
1	316.0
2	234.0
3	234.0
4	791.0
5	1348.0
6	1348.0
7	1508.5
8	1669.0
9	1612.5
10	1556.0
11	1556.0
12	1947.0
13	2338.0
14	2845.5
15	3353.0
16	3353.0
17	4983.0
18	6613.0
19	6613.0
20	7423.5
21	8234.0
22	7888.0
23	7542.0
24	7542.0
25	7670.0
26	7798.0
27	7798.0
28	9933.5
29	12069.0
30	14872.0
31	17675.0
32	17675.0
33	23884.5
34	30094.0
35	30094.0
36	33005.0
37	35916.0
38	41655.0
39	47394.0
40	47394.0
41	51133.0
42	54872.0
43	64255.0
44	73638.0
45	73638.0
46	75710.0
47	77782.0
48	77782.0
49	83538.0
50	89294.0
51	89368.0
52	89442.0
53	89442.0
54	84673.5
55	79905.0
56	79905.0
57	78872.0
58	77839.0
59	70061.5
60	62284.0
61	62284.0
62	60749.5
63	59215.0
64	48554.5
65	37894.0
66	37894.0
67	31989.5
68	26085.0
69	26085.0
70	21189.5
71	16294.0
72	12711.0
73	9128.0
74	9128.0
75	6784.0
76	4440.0
77	4440.0
78	4578.5
79	4717.0
80	3652.5
81	2588.0
82	2588.0
83	2574.0
84	2560.0
85	2560.0
86	1597.0
87	634.0
88	547.5
89	461.0
90	461.0
91	273.5
92	86.0
93	60.5
94	35.0
95	35.0
96	23.0
97	11.0
98	11.0
99	17.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009753969236190792
2	0.0
3	0.0
4	1.0488138963646013E-4
5	1.0488138963646013E-4
6	1.0488138963646013E-4
7	3.146441689093804E-4
8	0.0013634580652739817
9	0.003041560299457344
10	9.439325067281412E-4
11	0.005558713650732386
12	3.146441689093804E-4
13	1.0488138963646013E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0488138963646013E-4
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0488138963646013E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	953458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.527275837188576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.24831780503135	37.18929384115718
2	13.056006790270505	12.410328721067867
3	3.8589081646349053	5.502101783129468
4	1.6242684737656492	3.087882231452372
5	0.8328851000746879	1.979237994596705
6	0.50672124291307	1.4449848170695507
7	0.3319948460061858	1.1045167435862655
8	0.22177453415427917	0.843227156673154
9	0.1860417051857781	0.7957849895626852
>10	0.864587925773355	7.681721199659046
>50	0.10859760396779511	3.618525644221051
>100	0.13568935948732502	13.753669479512443
>500	0.017322045883854296	5.532160971941709
>1k	0.00666232533994396	4.527771995542325
>5k	2.2207751133146534E-4	0.528792430828175
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5010	0.5254557620786652	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3492	0.36624581261051875	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2084	0.21857281600238287	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1790	0.18773768744926364	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1702	0.17850812516125514	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1633	0.17127130927633938	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1532	0.1606782889230569	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1521	0.15952459363705584	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1472	0.1543854055448693	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1469	0.15407076137595993	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1464	0.15354635442777761	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1449	0.15197313358323072	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1385	0.14526072464649728	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1366	0.14326797824340454	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1344	0.1409605876714024	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1331	0.1395971296061284	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1327	0.1391776040475826	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1295	0.13582139957921585	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1278	0.13403841595539603	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1273	0.13351400900721372	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1263	0.13246519511084914	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1259	0.13204566955230332	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1221	0.1280601767461178	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1211	0.12701136284975323	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1195	0.12533326061556985	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1173	0.12302587004356773	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1171	0.1228161072642948	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1084	0.11369142636592279	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1078	0.11306213802810403	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1025	0.10750342437737163	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1011	0.10603508492246119	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.0488138963646013E-4	0.0
16	0.0	0.0	0.0	2.0976277927292026E-4	0.0
17	0.0	0.0	0.0	2.0976277927292026E-4	0.0
18	0.0	0.0	0.0	3.146441689093804E-4	0.0
19	0.0	0.0	0.0	4.195255585458405E-4	0.0
20	0.0	0.0	0.0	4.195255585458405E-4	0.0
21	0.0	0.0	0.0	7.341697274552209E-4	0.0
22	0.0	0.0	0.0	9.439325067281412E-4	0.0
23	0.0	0.0	0.0	0.0019927464030927424	0.0
24	0.0	0.0	0.0	0.0026220347409115032	0.0
25	0.0	0.0	0.0	0.0037757300269125648	0.0
26	0.0	0.0	0.0	0.004929425312913626	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGTT	35	0.002064394	22.86074	9
TATGGGT	55	5.8343096E-5	20.362568	3
TAAGACT	65	9.435322E-6	19.691275	4
CGATAAC	145	0.0	18.761572	10
ACGAACG	145	0.0	18.757637	15
CGAACGA	140	1.8189894E-12	18.284756	16
CCGATAA	155	0.0	17.551147	9
TAACGAA	155	1.0913936E-11	16.515263	13
ATAACGA	165	1.8189894E-12	16.484848	12
ACCCCGC	60	0.0024389331	16.002518	6
ACTATAC	80	5.621767E-5	15.999161	3
TATTAGA	130	4.800313E-9	15.999161	2
CGGTCCA	405	0.0	15.804956	10
ATACCGT	405	0.0	15.804956	6
CGCCGGT	395	0.0	15.799955	7
TACCGTC	375	0.0	15.789151	7
CGTCGTA	360	0.0	15.558002	10
TCAGATA	360	0.0	15.55474	2
GATAACG	175	3.6379788E-12	15.545303	11
CCGGTCC	425	0.0	15.437723	9
>>END_MODULE