FastQCFastQC Report
Thu 2 Feb 2017
SRR4063050_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063050_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990280
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27720.2799208304721897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18230.18408934846710023No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11540.11653269782283798No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11510.11622975320111484No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11120.1122914731187139No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10870.10976693460435433No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10790.10895908227975926No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10410.1051217837379327No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10290.1039100052510401No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10000.10098154057438301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9980.10077957749323424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC1650.021.3434371
AAGACGG2800.018.8546435
ATCGTTT2800.018.28513729
GTCCTAA2250.017.78621
CATCGTT2700.017.77721628
TCGTTTA2450.017.63209730
GCGCAAG2600.017.2389321
CGCAAGA2600.017.2319662
CGGCATC2850.016.84242425
GCATCGT2850.016.84157427
CGGACCA3000.016.5336469
CGTTTAT2650.016.30137331
ACGGACC3050.016.2609628
GTCTAGT908.539913E-616.0075781
CCTTATA703.6937982E-416.001112
GTAGAAC2500.015.9994953
CAAGACG3000.015.9978784
ATACACT2350.015.6574994
GGCATCG3200.015.50029326
GACGGAC3200.015.4987277
Produced by FastQC (version 0.11.5)