##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063050_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	990280
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21212384376136	32.0	32.0	32.0	32.0	32.0
2	30.812789312113747	32.0	32.0	32.0	32.0	32.0
3	30.86072827887062	32.0	32.0	32.0	32.0	32.0
4	30.93361271559559	32.0	32.0	32.0	32.0	32.0
5	30.83405602455871	32.0	32.0	32.0	32.0	32.0
6	34.48741870985984	36.0	36.0	36.0	32.0	36.0
7	34.413077109504385	36.0	36.0	36.0	32.0	36.0
8	34.387459102476065	36.0	36.0	36.0	32.0	36.0
9	34.54716847760229	36.0	36.0	36.0	32.0	36.0
10	34.24849436523004	36.0	36.0	36.0	32.0	36.0
11	34.537117784868926	36.0	36.0	36.0	32.0	36.0
12	34.34322009936584	36.0	36.0	36.0	32.0	36.0
13	34.438283111847156	36.0	36.0	36.0	32.0	36.0
14	34.33097103849416	36.0	36.0	36.0	32.0	36.0
15	34.27018822959163	36.0	36.0	36.0	32.0	36.0
16	34.28611705780183	36.0	36.0	36.0	32.0	36.0
17	34.21705174294139	36.0	36.0	36.0	32.0	36.0
18	34.25134608393586	36.0	36.0	36.0	32.0	36.0
19	34.23060447550188	36.0	36.0	36.0	32.0	36.0
20	34.21592074968696	36.0	36.0	36.0	32.0	36.0
21	34.20529345235691	36.0	36.0	36.0	32.0	36.0
22	34.16608837904431	36.0	36.0	36.0	32.0	36.0
23	34.115104818839114	36.0	36.0	36.0	32.0	36.0
24	34.0942339540332	36.0	36.0	36.0	32.0	36.0
25	34.083019953952416	36.0	36.0	36.0	32.0	36.0
26	34.034175182776586	36.0	36.0	36.0	32.0	36.0
27	34.03879003918084	36.0	36.0	36.0	32.0	36.0
28	34.02903926162298	36.0	36.0	36.0	32.0	36.0
29	33.97643797713778	36.0	36.0	36.0	32.0	36.0
30	33.95383931817264	36.0	36.0	36.0	32.0	36.0
31	33.96306600153492	36.0	36.0	36.0	32.0	36.0
32	33.92907763460839	36.0	36.0	36.0	32.0	36.0
33	33.88073979076625	36.0	36.0	36.0	32.0	36.0
34	33.895843599789956	36.0	36.0	36.0	32.0	36.0
35	33.86231368905764	36.0	36.0	36.0	32.0	36.0
36	33.79998384295351	36.0	36.0	36.0	32.0	36.0
37	33.79780668093873	36.0	36.0	36.0	32.0	36.0
38	33.19764713010462	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	2.0
9	4.0
10	7.0
11	10.0
12	3.0
13	5.0
14	163.0
15	352.0
16	423.0
17	472.0
18	602.0
19	719.0
20	962.0
21	1310.0
22	1825.0
23	2908.0
24	4355.0
25	6550.0
26	9537.0
27	13620.0
28	19305.0
29	26417.0
30	35896.0
31	48952.0
32	67193.0
33	100810.0
34	222432.0
35	425443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28704788731825	17.565626039506558	11.416398143270671	25.730927929904524
2	16.415499069930515	20.010386870510686	38.07778230210637	25.496331757452428
3	18.90678858795714	24.117429028215	28.78724068244412	28.188541701383734
4	12.523137979611313	15.44763722475524	36.39688767034754	35.632337125285915
5	14.359791533497127	36.74469518186289	33.83783761949915	15.057675665140835
6	33.706022539079854	35.85834309488226	17.102940582461525	13.332693783576364
7	29.834188310376863	30.80401502605324	21.10312234923456	18.25867431433534
8	27.74363459002256	33.38853690998653	19.72938802795994	19.138440472030975
9	27.57056863887579	14.29226367975829	18.86260107020561	39.27456661116031
10	15.870036101083032	26.77862209992174	31.927292923682813	25.424048875312412
11	37.334891179654555	21.218452602155402	22.5100378084051	18.936618409784945
12	24.767888772420925	23.949314219122694	28.959018760767396	22.323778247688985
13	29.65061788742693	19.54616885556503	25.279352177654253	25.523861079353793
14	23.364532228647374	20.13106363726336	25.37502297501732	31.12938115907195
15	24.931115285942266	27.666303052340265	22.63802193806436	24.76455972365311
16	25.39166844242552	25.825020930389915	24.41064162627867	24.372669000905887
17	23.692271953701415	26.065377564779133	25.678646998520342	24.563703482999106
18	24.757759521729692	24.808454558766353	26.860694683585212	23.573091235918746
19	25.367721685808895	25.18099531517775	25.724814409741683	23.726468589271665
20	25.496197853024043	24.52844288700605	25.55234642456803	24.423012835401874
21	26.609534812325293	24.3999337523631	24.798429446266702	24.192101989044904
22	25.629463708634955	24.789135211995863	25.245100019389866	24.336301059979316
23	24.00370432834811	24.58258137555002	25.89969834163643	25.514015954465442
24	24.529319444696927	25.326663717654284	25.62217091748623	24.521845920162562
25	24.623108472648788	24.860044902786377	25.658417656510853	24.858428968053982
26	24.077240604746383	25.85322103502572	26.18561136692115	23.88392699330674
27	25.03602832983065	25.133989030421343	25.318902476587894	24.511080163160113
28	24.064570205202777	25.171372596542252	26.03732428502181	24.726732913233153
29	24.208197120312832	25.24437550054182	25.93091942147717	24.616507957668173
30	24.015680841158773	25.442184976970534	26.273487684460434	24.268646497410256
31	24.857734640643002	25.136862557777157	25.03345192566957	24.97195087591027
32	24.205178232759927	25.41773460900687	25.12113445267829	25.25595270555492
33	23.870746870367153	25.039486493801327	25.88284125015653	25.20692538567499
34	24.753375184817124	25.131189858690643	25.992962810351372	24.122472146140858
35	25.412290597582764	25.00083321634639	25.83738242812373	23.749493757947114
36	24.06969392763579	25.738316511102266	25.51380688738565	24.67818267387629
37	24.93932988686021	25.536588395572167	25.23806013183315	24.286021585734467
38	24.290441907881913	25.37806911330611	25.76919076294473	24.56229821586724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	563.0
1	400.0
2	237.0
3	237.0
4	537.0
5	837.0
6	837.0
7	930.5
8	1024.0
9	1002.0
10	980.0
11	980.0
12	1225.5
13	1471.0
14	1956.5
15	2442.0
16	2442.0
17	3644.0
18	4846.0
19	4846.0
20	5857.5
21	6869.0
22	7426.5
23	7984.0
24	7984.0
25	9641.5
26	11299.0
27	11299.0
28	14546.5
29	17794.0
30	21973.5
31	26153.0
32	26153.0
33	32509.5
34	38866.0
35	38866.0
36	43029.5
37	47193.0
38	53037.0
39	58881.0
40	58881.0
41	61587.0
42	64293.0
43	71055.5
44	77818.0
45	77818.0
46	79574.0
47	81330.0
48	81330.0
49	85685.0
50	90040.0
51	90222.0
52	90404.0
53	90404.0
54	85151.5
55	79899.0
56	79899.0
57	77071.5
58	74244.0
59	66052.0
60	57860.0
61	57860.0
62	54871.0
63	51882.0
64	43066.5
65	34251.0
66	34251.0
67	28744.5
68	23238.0
69	23238.0
70	18750.5
71	14263.0
72	11177.0
73	8091.0
74	8091.0
75	6249.0
76	4407.0
77	4407.0
78	4197.5
79	3988.0
80	3123.0
81	2258.0
82	2258.0
83	2253.5
84	2249.0
85	2249.0
86	1488.0
87	727.0
88	636.5
89	546.0
90	546.0
91	404.0
92	262.0
93	228.0
94	194.0
95	194.0
96	171.5
97	149.0
98	149.0
99	298.5
100	448.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1241063133659167
2	0.05745849658682393
3	0.013430544896392938
4	0.002524538514359575
5	1.00981540574383E-4
6	0.0
7	0.0
8	6.05889243446298E-4
9	3.02944621723149E-4
10	5.04907702871915E-4
11	0.002423556973785192
12	0.002221593892636426
13	0.013329563355818556
14	0.007472634002504342
15	0.02221593892636426
16	0.009391283273417619
17	0.018681585006260857
18	0.004544169325847235
19	0.006159873975037363
20	0.00504907702871915
21	0.005654966272165448
22	0.007270670921355577
23	0.00878539402997132
24	0.011915821787777194
25	0.014238397220988004
26	0.018075695762814557
27	0.00878539402997132
28	0.00403926162297532
29	0.010401098679161449
30	0.002726501595508341
31	0.005352021650442299
32	0.005856929353314214
33	0.006866744759058044
34	0.011309932544330896
35	0.014238397220988004
36	0.012319747950074726
37	0.008987357111120088
38	0.005150058569293533
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	990280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.0726919184366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.19776076644216	54.58849877728913
2	10.127234880030164	12.97758400907636
3	2.2955859767575695	4.412531191832134
4	0.8280865440111566	2.122309360649189
5	0.4103550348800673	1.314627586352493
6	0.23007510791309063	0.8844918904449905
7	0.14062851527890724	0.6307313274088805
8	0.10736599159246517	0.550338248145718
9	0.0828562659671034	0.47779416025400334
>10	0.43969792590709805	5.538054844572337
>50	0.06171665050965705	2.8500776019778966
>100	0.07104168441140303	9.593821973989067
>500	0.006170658243132956	2.8174723243355184
>1k	0.0014239980561076054	1.2416667036723312
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2772	0.2799208304721897	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1823	0.18408934846710023	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1154	0.11653269782283798	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1151	0.11622975320111484	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1112	0.1122914731187139	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1087	0.10976693460435433	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1079	0.10895908227975926	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1041	0.1051217837379327	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1029	0.1039100052510401	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1000	0.10098154057438301	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	998	0.10077957749323424	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.00981540574383E-4	0.0
8	0.0	0.0	0.0	2.01963081148766E-4	0.0
9	0.0	0.0	0.0	2.01963081148766E-4	0.0
10	0.0	0.0	0.0	3.02944621723149E-4	0.0
11	0.0	0.0	0.0	3.02944621723149E-4	0.0
12	0.0	0.0	0.0	3.02944621723149E-4	0.0
13	0.0	0.0	0.0	3.02944621723149E-4	0.0
14	0.0	0.0	0.0	5.04907702871915E-4	1.00981540574383E-4
15	0.0	0.0	0.0	6.05889243446298E-4	1.00981540574383E-4
16	0.0	0.0	0.0	6.05889243446298E-4	1.00981540574383E-4
17	0.0	0.0	0.0	7.06870784020681E-4	2.01963081148766E-4
18	0.0	0.0	0.0	8.07852324595064E-4	2.01963081148766E-4
19	0.0	0.0	0.0	9.08833865169447E-4	2.01963081148766E-4
20	0.0	0.0	0.0	0.00100981540574383	2.01963081148766E-4
21	0.0	0.0	0.0	0.001312760027466979	2.01963081148766E-4
22	0.0	0.0	0.0	0.002221593892636426	2.01963081148766E-4
23	0.0	0.0	0.0	0.003130427757805873	3.02944621723149E-4
24	0.0	0.0	0.0	0.003837298541826554	3.02944621723149E-4
25	0.0	0.0	0.0	0.004241224704124086	3.02944621723149E-4
26	0.0	0.0	0.0	0.005352021650442299	3.02944621723149E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	165	0.0	21.343437	1
AAGACGG	280	0.0	18.854643	5
ATCGTTT	280	0.0	18.285137	29
GTCCTAA	225	0.0	17.7862	1
CATCGTT	270	0.0	17.777216	28
TCGTTTA	245	0.0	17.632097	30
GCGCAAG	260	0.0	17.238932	1
CGCAAGA	260	0.0	17.231966	2
CGGCATC	285	0.0	16.842424	25
GCATCGT	285	0.0	16.841574	27
CGGACCA	300	0.0	16.533646	9
CGTTTAT	265	0.0	16.301373	31
ACGGACC	305	0.0	16.260962	8
GTCTAGT	90	8.539913E-6	16.007578	1
CCTTATA	70	3.6937982E-4	16.00111	2
GTAGAAC	250	0.0	15.999495	3
CAAGACG	300	0.0	15.997878	4
ATACACT	235	0.0	15.657499	4
GGCATCG	320	0.0	15.500293	26
GACGGAC	320	0.0	15.498727	7
>>END_MODULE