FastQCFastQC Report
Thu 2 Feb 2017
SRR4063050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990280
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30820.31122510805024844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22860.23084380175303953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13550.13682998747828898No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12580.12703477804257382No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12210.12329846104132164No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11760.1187542917154744No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10960.11067576846952376No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10800.10906006382033365No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10770.1087571191986105No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10430.10532374681908148No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10320.10421294987276326No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10160.10259724522357312No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9920.10017368824978795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC400.00447912120.0030298
TAGCGGC2800.019.4275930
TACCGTC1750.019.202917
TCTAGCG2750.019.1990328
CTAGCGG2750.019.1990329
ATACCGT1950.018.8746556
ATCGTTT2050.018.7307629
ACCGTCG1850.018.1649138
CGTCGTA1950.018.05219510
CCGTCGT1950.017.233389
CGGCATC2450.016.97873325
GCATCGT2200.016.72642927
CCAACGA1354.4383341E-1016.59175529
CGGTCCA3400.016.4714210
CCGGTCC3500.016.0024249
TAGTATC600.002439117816.0024247
GCATATA908.566067E-616.0024241
AGCGGCG3550.015.77385131
CATCGTT2350.015.65878428
CAAGACG3200.015.5015654
Produced by FastQC (version 0.11.5)