##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063050_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	990280
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.167062850910852	32.0	32.0	32.0	32.0	32.0
2	31.260436442218364	32.0	32.0	32.0	32.0	32.0
3	31.346439390879347	32.0	32.0	32.0	32.0	32.0
4	31.444581330532777	32.0	32.0	32.0	32.0	32.0
5	31.367609161045362	32.0	32.0	32.0	32.0	32.0
6	34.887896352546754	36.0	36.0	36.0	36.0	36.0
7	34.911842105263155	36.0	36.0	36.0	36.0	36.0
8	34.85344750979521	36.0	36.0	36.0	32.0	36.0
9	34.96353556569859	36.0	36.0	36.0	32.0	36.0
10	34.80946499979804	36.0	36.0	36.0	32.0	36.0
11	34.98679868320071	36.0	36.0	36.0	36.0	36.0
12	34.8841590257301	36.0	36.0	36.0	32.0	36.0
13	34.94093286747183	36.0	36.0	36.0	36.0	36.0
14	34.88394090560246	36.0	36.0	36.0	32.0	36.0
15	34.85190451185523	36.0	36.0	36.0	32.0	36.0
16	34.8683725814921	36.0	36.0	36.0	32.0	36.0
17	34.83703094074403	36.0	36.0	36.0	32.0	36.0
18	34.828313204346244	36.0	36.0	36.0	32.0	36.0
19	34.82544229914772	36.0	36.0	36.0	32.0	36.0
20	34.8178444480349	36.0	36.0	36.0	32.0	36.0
21	34.80041200468554	36.0	36.0	36.0	32.0	36.0
22	34.785459667972695	36.0	36.0	36.0	32.0	36.0
23	34.725044431877855	36.0	36.0	36.0	32.0	36.0
24	34.70327685099164	36.0	36.0	36.0	32.0	36.0
25	34.68025305974068	36.0	36.0	36.0	32.0	36.0
26	34.62407500908834	36.0	36.0	36.0	32.0	36.0
27	34.60679302823444	36.0	36.0	36.0	32.0	36.0
28	34.5690400694753	36.0	36.0	36.0	32.0	36.0
29	34.54059054004928	36.0	36.0	36.0	32.0	36.0
30	34.51328008240094	36.0	36.0	36.0	32.0	36.0
31	34.50316072221998	36.0	36.0	36.0	32.0	36.0
32	34.470404330088456	36.0	36.0	36.0	32.0	36.0
33	34.43854566385265	36.0	36.0	36.0	32.0	36.0
34	34.42114755422709	36.0	36.0	36.0	32.0	36.0
35	34.3989629195783	36.0	36.0	36.0	32.0	36.0
36	34.36390010906006	36.0	36.0	36.0	32.0	36.0
37	34.346467665710705	36.0	36.0	36.0	32.0	36.0
38	33.91593387728723	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	6.0
21	18.0
22	63.0
23	221.0
24	633.0
25	1611.0
26	3476.0
27	6610.0
28	11994.0
29	18913.0
30	28341.0
31	41272.0
32	58913.0
33	94866.0
34	228640.0
35	494703.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.54053759270929	18.76353272900609	12.160469888668056	26.53545978961656
2	15.372015995476026	21.04061477561902	37.29965262350042	26.287716605404533
3	17.900997697620873	24.889122268449327	29.065314860443507	28.144565173486285
4	11.960770651503264	16.377909659802945	36.492140093852335	35.16917959484145
5	13.90606697095771	37.39104091772024	33.41166134830553	15.29123076301652
6	33.739986407870916	36.34763536336729	16.656518011590673	13.25586021717112
7	29.547508616310857	30.992059765337437	21.055405933515303	18.405025684836403
8	27.621780902427844	33.66694874689728	19.544019663483	19.167250687191874
9	27.382470767276313	14.038069443560822	18.682585991519254	39.89687379764361
10	15.636379426300195	27.032013361958406	31.941967576650505	25.389639635090894
11	37.41269546319374	21.087100540086364	22.523661599738237	18.976542396981657
12	24.609352121323507	24.072028258731386	28.917637269534413	22.400982350410693
13	29.644866683631633	19.59958637793908	25.299209513307403	25.456337425121884
14	23.411358403683806	20.032415074524376	25.316274185078967	31.23995233671285
15	24.853576766167144	27.80536817869693	22.48091448883144	24.86014056630448
16	25.247733214578922	25.97490202256132	24.302242095409476	24.475122667450282
17	23.650684654845094	26.209051985297087	25.56398190410793	24.57628145574989
18	24.72007916952781	24.772084662923618	26.88764793795694	23.62018822959163
19	25.36262471220261	25.064224259805307	25.787050127236743	23.78610090075534
20	25.517227450821988	24.45883992406188	25.534394312719634	24.489538312396494
21	26.6488804153985	24.470300259119156	24.7745582553586	24.106261070123743
22	25.48804378559599	24.84236781516339	25.343539201034048	24.32604919820657
23	23.87039181966258	24.49254097590876	25.983134011998665	25.653933192429996
24	24.51155228824171	25.332835157733168	25.601950963363894	24.55366159066123
25	24.617380942763663	24.7343175667488	25.811790604677466	24.836510885810075
26	24.17033566264087	25.626186533101748	26.22874338570909	23.974734418548287
27	25.066698442255607	24.97753156184425	25.292998531725434	24.662771464174707
28	24.097426990346165	25.012420729490646	26.16239851355172	24.727753766611464
29	24.16710425334249	25.05503493961304	26.076059296360626	24.701801510683847
30	24.027345801187543	25.297390636991558	26.291957830108657	24.383305731712245
31	24.736438179100862	24.9877812335905	25.329502766894212	24.946277820414426
32	24.320394231934404	25.2416488265945	25.254473482247448	25.183483459223655
33	23.90586500787656	24.847012966029812	26.003049642525344	25.244072383568284
34	24.813790039180837	24.920426546027386	26.095851678313203	24.169931736478574
35	25.4217998949792	24.79884477117583	25.954376539968493	23.82497879387648
36	24.156804136203903	25.54035222361352	25.551662156157857	24.75118148402472
37	25.120571959445815	25.313244738861734	25.315769277376095	24.250414024316356
38	24.376842913115482	25.138546673668056	25.803914044512666	24.6806963687038
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	273.0
1	203.5
2	134.0
3	134.0
4	455.0
5	776.0
6	776.0
7	873.5
8	971.0
9	952.0
10	933.0
11	933.0
12	1240.0
13	1547.0
14	1992.0
15	2437.0
16	2437.0
17	3762.5
18	5088.0
19	5088.0
20	6070.5
21	7053.0
22	7693.0
23	8333.0
24	8333.0
25	9898.5
26	11464.0
27	11464.0
28	14627.0
29	17790.0
30	21752.0
31	25714.0
32	25714.0
33	32720.5
34	39727.0
35	39727.0
36	43464.0
37	47201.0
38	52667.5
39	58134.0
40	58134.0
41	61115.5
42	64097.0
43	71152.5
44	78208.0
45	78208.0
46	79913.5
47	81619.0
48	81619.0
49	86084.0
50	90549.0
51	90411.0
52	90273.0
53	90273.0
54	85162.0
55	80051.0
56	80051.0
57	77501.5
58	74952.0
59	66713.5
60	58475.0
61	58475.0
62	55320.5
63	52166.0
64	43054.0
65	33942.0
66	33942.0
67	28603.0
68	23264.0
69	23264.0
70	18704.5
71	14145.0
72	10981.5
73	7818.0
74	7818.0
75	5961.5
76	4105.0
77	4105.0
78	4063.0
79	4021.0
80	3091.5
81	2162.0
82	2162.0
83	2021.0
84	1880.0
85	1880.0
86	1212.0
87	544.0
88	440.5
89	337.0
90	337.0
91	199.0
92	61.0
93	41.0
94	21.0
95	21.0
96	13.5
97	6.0
98	6.0
99	7.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008886375570545705
2	0.0
3	0.0
4	1.00981540574383E-4
5	0.0
6	1.00981540574383E-4
7	7.06870784020681E-4
8	0.0018176677303388942
9	0.003736317001252171
10	6.05889243446298E-4
11	0.006866744759058044
12	2.01963081148766E-4
13	4.03926162297532E-4
14	0.0
15	0.0
16	1.00981540574383E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	2.01963081148766E-4
22	0.0
23	3.02944621723149E-4
24	0.0
25	0.0
26	0.0
27	2.01963081148766E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	990280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.5730681822149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.33911768657059	52.77357361429627
2	10.701679915719724	13.392738940611402
3	2.450973094862362	4.600947197327905
4	0.8795708028858269	2.2014977528024136
5	0.43454482006996176	1.359540132723301
6	0.2516898227348051	0.9449402663252723
7	0.16435462997080777	0.7198921427058237
8	0.10740922297527183	0.5376739706104326
9	0.0739133000877263	0.41624837693656264
>10	0.4447536507951834	5.474226535149878
>50	0.06674600616917266	2.969803313477959
>100	0.07615402909784809	9.914036108644787
>500	0.007144514190686988	2.9958725823314265
>1k	0.0019485038701873606	1.699009066056641
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3082	0.31122510805024844	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2286	0.23084380175303953	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1355	0.13682998747828898	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1258	0.12703477804257382	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1221	0.12329846104132164	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1176	0.1187542917154744	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1096	0.11067576846952376	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1080	0.10906006382033365	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1077	0.1087571191986105	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1043	0.10532374681908148	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1032	0.10421294987276326	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1016	0.10259724522357312	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	992	0.10017368824978795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.02944621723149E-4
2	0.0	0.0	0.0	0.0	3.02944621723149E-4
3	0.0	0.0	0.0	0.0	3.02944621723149E-4
4	0.0	0.0	0.0	0.0	3.02944621723149E-4
5	0.0	0.0	0.0	0.0	3.02944621723149E-4
6	0.0	0.0	0.0	0.0	3.02944621723149E-4
7	0.0	0.0	0.0	1.00981540574383E-4	3.02944621723149E-4
8	0.0	0.0	0.0	1.00981540574383E-4	3.02944621723149E-4
9	0.0	0.0	0.0	1.00981540574383E-4	3.02944621723149E-4
10	0.0	0.0	0.0	1.00981540574383E-4	3.02944621723149E-4
11	0.0	0.0	0.0	1.00981540574383E-4	3.02944621723149E-4
12	0.0	0.0	0.0	1.00981540574383E-4	3.02944621723149E-4
13	0.0	0.0	0.0	1.00981540574383E-4	3.02944621723149E-4
14	0.0	0.0	0.0	1.00981540574383E-4	4.03926162297532E-4
15	0.0	0.0	0.0	3.02944621723149E-4	4.03926162297532E-4
16	0.0	0.0	0.0	3.02944621723149E-4	4.03926162297532E-4
17	0.0	0.0	0.0	4.03926162297532E-4	5.04907702871915E-4
18	0.0	0.0	0.0	5.04907702871915E-4	5.04907702871915E-4
19	0.0	0.0	0.0	6.05889243446298E-4	5.04907702871915E-4
20	0.0	0.0	0.0	7.06870784020681E-4	5.04907702871915E-4
21	0.0	0.0	0.0	0.00100981540574383	5.04907702871915E-4
22	0.0	0.0	0.0	0.00201963081148766	5.04907702871915E-4
23	0.0	0.0	0.0	0.002928464676657107	7.06870784020681E-4
24	0.0	0.0	0.0	0.003736317001252171	7.06870784020681E-4
25	0.0	0.0	0.0	0.004241224704124086	7.06870784020681E-4
26	0.0	0.0	0.0	0.005251040109867916	7.06870784020681E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTTC	40	0.004479121	20.003029	8
TAGCGGC	280	0.0	19.42759	30
TACCGTC	175	0.0	19.20291	7
TCTAGCG	275	0.0	19.19903	28
CTAGCGG	275	0.0	19.19903	29
ATACCGT	195	0.0	18.874655	6
ATCGTTT	205	0.0	18.73076	29
ACCGTCG	185	0.0	18.164913	8
CGTCGTA	195	0.0	18.052195	10
CCGTCGT	195	0.0	17.23338	9
CGGCATC	245	0.0	16.978733	25
GCATCGT	220	0.0	16.726429	27
CCAACGA	135	4.4383341E-10	16.591755	29
CGGTCCA	340	0.0	16.47142	10
CCGGTCC	350	0.0	16.002424	9
TAGTATC	60	0.0024391178	16.002424	7
GCATATA	90	8.566067E-6	16.002424	1
AGCGGCG	355	0.0	15.773851	31
CATCGTT	235	0.0	15.658784	28
CAAGACG	320	0.0	15.501565	4
>>END_MODULE