FastQCFastQC Report
Thu 2 Feb 2017
SRR4063049_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063049_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690489
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT84461.2231911007995784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT54950.7958128225069481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT28230.40884069116235017No Hit
CCCTATTAGTGTGTAGTGAACAACGAAGCATGAATGGG28120.40724761726834174No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA27940.40464076907814606No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCG27820.4029028702846823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.3824825594614831No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGT25830.3740827152930749No Hit
CTATTAGTGTGTAGTGAACAACGAAGCATGAATGGGTT25340.3669862952197645No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAA24400.3533727546709651No Hit
ATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTC23780.34439361090473564No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTA19800.28675330092152085No Hit
CACTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTC19490.28226372903840613No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGAT18570.26893983828851725No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAG17190.24895400216368402No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTT16250.23534046161488453No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACA16170.23418186241924202No Hit
GTACATGGGACACGACCGTACCGCAAACCGACACTGGT15260.2210027965688085No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA15240.22071314676989787No Hit
GTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGC15230.22056832187044256No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA14470.2095616295118387No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGG14060.20362380863417087No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCC14020.20304450903634957No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCA13900.20130661024288585No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGG13250.19189299177829044No Hit
AGTGTGTAGTGAACAACGAAGCATGAATGGGTTGCAAA13020.18856201909081824No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCAC12840.1859551709006226No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTT12530.18146559901750786No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA11710.1695899572621722No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATT11480.16625898457469998No Hit
GTATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAAC11080.16046598859648742No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10940.15843844000411303No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA10380.15032824563461547No Hit
CTACACACTAATAGGGCACACCTTCTTCCGAAGTTACG10050.14554902395259012No Hit
GACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGAT9540.13816295408036913No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA9490.13743882958309256No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.13425268179507568No Hit
GTATCAACGCAGAGTACATGGGACACGACCGTACCGCA9120.13208030830324596No Hit
GTAGTGAACAACGAAGCATGAATGGGTTGCAAAAAATC8990.13019758461032688No Hit
CGACCGTACCGCAAACCGACACTGGTGCGCGAGATGAG8910.1290389854146844No Hit
TATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCCG8630.1249838882299356No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT8530.12353563923538245No Hit
CATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGTGTG8400.1216529155424634No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCAGGAGGAG8320.12049431634682088No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACT8300.12020466654791023No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT7750.11223929707786799No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA7720.11180482237950207No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA7630.11050139828440425No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT7460.10803937499366391No Hit
CCTTAGGACCGTTATCGTTACGGCCGCCGTTTACTGGG7460.10803937499366391No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA7450.10789455009420859No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7420.10746007539584265No Hit
AACCGACACTGGTGCGCGAGATGAGTATTCTAAGGCGC7170.10383945290945983No Hit
ACGCAGAGTACATGGGGTCTTTCCGTCTTTCCGCGGGG7110.10297050351272795No Hit
GAGTATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGA7100.10282567861327262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAAC200.003755939231.993994
TACCGAG557.5928256E-826.1806937
GCTTAGT653.8135295E-722.15289317
TATACCG604.7178128E-621.3324175
CTAGGAC453.5375517E-421.330873
TCCTACA3650.020.6137982
CCTACAG3200.020.4976353
GTCCGTC555.830897E-520.362767
CTTAATC555.8341488E-520.3612863
CATAGTC555.8374022E-520.3598144
ACCGAGG801.264234E-719.999148
CTCTCGG400.004482812219.9991415
GTAGACC400.004484691719.9976923
CGGGAGT400.00448657219.9962434
TCGGGTA4000.019.59915725
ACCGTTA6150.019.5113548
GTCCTAT507.145091E-419.2186621
TCTCGAT507.1940373E-419.19917524
CCGACTA507.1940373E-419.1991759
AAGCTTA1003.25781E-919.19917515
Produced by FastQC (version 0.11.5)