##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063049_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690489
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.963337576702887	32.0	32.0	32.0	32.0	32.0
2	30.702746893867968	32.0	32.0	32.0	32.0	32.0
3	30.80330461455577	32.0	32.0	32.0	32.0	32.0
4	30.805940427725858	32.0	32.0	32.0	32.0	32.0
5	30.85562695423099	32.0	32.0	32.0	32.0	32.0
6	34.409266476366746	36.0	36.0	36.0	32.0	36.0
7	34.24966943716699	36.0	36.0	36.0	32.0	36.0
8	34.158807743497725	36.0	36.0	36.0	32.0	36.0
9	34.24369396181547	36.0	36.0	36.0	32.0	36.0
10	34.11469263087464	36.0	36.0	36.0	32.0	36.0
11	34.41022087245416	36.0	36.0	36.0	32.0	36.0
12	34.252593451886995	36.0	36.0	36.0	32.0	36.0
13	34.32187768378642	36.0	36.0	36.0	32.0	36.0
14	34.22054080513955	36.0	36.0	36.0	32.0	36.0
15	34.23910301250273	36.0	36.0	36.0	32.0	36.0
16	34.23116805626157	36.0	36.0	36.0	32.0	36.0
17	34.16318869670625	36.0	36.0	36.0	32.0	36.0
18	34.24703217574791	36.0	36.0	36.0	32.0	36.0
19	34.15538553112359	36.0	36.0	36.0	32.0	36.0
20	34.05190234746679	36.0	36.0	36.0	32.0	36.0
21	34.01702271868198	36.0	36.0	36.0	32.0	36.0
22	34.003141252069184	36.0	36.0	36.0	32.0	36.0
23	34.03929968471619	36.0	36.0	36.0	32.0	36.0
24	34.01683299806369	36.0	36.0	36.0	32.0	36.0
25	34.021713597175335	36.0	36.0	36.0	32.0	36.0
26	34.022501444628375	36.0	36.0	36.0	32.0	36.0
27	34.021397878894525	36.0	36.0	36.0	32.0	36.0
28	34.031962855309786	36.0	36.0	36.0	32.0	36.0
29	33.97358683483734	36.0	36.0	36.0	32.0	36.0
30	33.92962523660768	36.0	36.0	36.0	32.0	36.0
31	33.94688257162677	36.0	36.0	36.0	32.0	36.0
32	33.88415745942368	36.0	36.0	36.0	32.0	36.0
33	33.79498442408207	36.0	36.0	36.0	32.0	36.0
34	33.746353671093964	36.0	36.0	36.0	27.0	36.0
35	33.69970991572639	36.0	36.0	36.0	27.0	36.0
36	33.64313406875417	36.0	36.0	36.0	27.0	36.0
37	33.65488660934497	36.0	36.0	36.0	27.0	36.0
38	33.03368192686632	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	8.0
11	11.0
12	4.0
13	1.0
14	274.0
15	515.0
16	531.0
17	670.0
18	970.0
19	1217.0
20	1493.0
21	1776.0
22	2199.0
23	2980.0
24	3855.0
25	5307.0
26	7308.0
27	9846.0
28	13697.0
29	18205.0
30	24533.0
31	32020.0
32	43511.0
33	63897.0
34	143360.0
35	312300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.578801447728644	18.76174539464526	11.91055519465454	23.748897962971554
2	15.822297828931447	20.406276580483713	40.371471089852356	23.39995450073248
3	19.34629768570957	28.717884838776026	28.266990727195303	23.668826748319105
4	11.43726528702871	16.13492818670154	38.18741120191869	34.240395324351056
5	12.244274322723415	40.02586583942325	32.70565949200998	15.024200345843363
6	28.138649766541924	38.99589855290751	18.760354995307665	14.105096685242902
7	27.91499937001169	33.5573774527907	20.38395977343593	18.143663403761682
8	28.837865393750505	32.45284494444508	18.492283718181923	20.217005943622482
9	30.98818373645344	14.28234193448411	18.99885443504531	35.73061989401714
10	17.8002160796888	25.26495830473029	30.73023928073849	26.204586334842418
11	35.091886286646975	24.38299542487541	22.078922709504933	18.446195578972674
12	24.76374753971576	25.826793092810828	29.053440487552663	20.35601887992075
13	28.563544714505056	22.584663518641907	27.329441756713695	21.522350010139345
14	23.42199981170745	22.553391800583707	26.094450439228577	27.93015794848026
15	24.26129194414546	29.042739822075553	25.920152746732185	20.775815487046806
16	23.40505966089322	28.22848137751629	25.684311459120917	22.682147502469576
17	21.764216759957815	26.350838461449317	26.533944071666145	25.351000706926722
18	21.546692466116497	26.062996231465096	29.716244673794346	22.674066628624065
19	23.60424949488366	26.47302063192046	29.562522178047175	20.36020769514871
20	24.22192386737403	25.20301888759988	28.593691388117655	21.981365856908436
21	27.4284886668115	23.11753204431892	26.446520385255994	23.007458903613585
22	24.97313268542792	24.47822371565546	27.696218298741364	22.852425300175252
23	23.795736520562784	25.48151710113525	29.387244323037216	21.33550205526475
24	22.57269248262536	26.617601473983665	27.636324658808125	23.17338138458285
25	23.88241309830245	26.640148678422072	27.633848966247506	21.84358925702797
26	22.812323907447567	26.00397199612361	29.346561731801902	21.837142364626928
27	23.083619025272693	27.134723483515323	28.12686025644925	21.654797234762736
28	22.657042053611256	26.549709465683353	28.15822099146646	22.635027489238936
29	22.34250728204207	26.069203661081957	28.82312640408345	22.765162652792522
30	22.74367133064627	26.406543957802597	28.915418360563617	21.934366350987517
31	22.12417065317987	28.152242078751193	28.597457031832725	21.12613023623621
32	21.823959399979433	28.192913824892134	27.39125460030734	22.59187217482109
33	22.399164581015942	27.721919030404152	27.49510451427512	22.383811874304783
34	23.167594625755356	26.906275166424297	28.69551740719926	21.230612800621085
35	22.496664827070738	27.072188930847123	29.684270296030096	20.74687594605204
36	23.358025835870663	27.027281497425975	27.617844654512584	21.996848012190775
37	23.279463054586746	28.198179669304658	26.696638587302875	21.825718688805722
38	22.537344993294266	28.672149049755667	27.312463972980094	21.478041983969966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	251.0
1	338.0
2	425.0
3	425.0
4	997.5
5	1570.0
6	1570.0
7	2093.0
8	2616.0
9	2810.5
10	3005.0
11	3005.0
12	3695.0
13	4385.0
14	5199.5
15	6014.0
16	6014.0
17	8300.5
18	10587.0
19	10587.0
20	11700.5
21	12814.0
22	11284.0
23	9754.0
24	9754.0
25	10248.5
26	10743.0
27	10743.0
28	14003.0
29	17263.0
30	21048.0
31	24833.0
32	24833.0
33	26611.0
34	28389.0
35	28389.0
36	29449.0
37	30509.0
38	36546.0
39	42583.0
40	42583.0
41	44693.0
42	46803.0
43	52574.0
44	58345.0
45	58345.0
46	60968.5
47	63592.0
48	63592.0
49	66290.5
50	68989.0
51	59623.5
52	50258.0
53	50258.0
54	51523.5
55	52789.0
56	52789.0
57	49411.5
58	46034.0
59	36501.5
60	26969.0
61	26969.0
62	25732.0
63	24495.0
64	20538.0
65	16581.0
66	16581.0
67	14102.0
68	11623.0
69	11623.0
70	9510.0
71	7397.0
72	5791.5
73	4186.0
74	4186.0
75	3448.5
76	2711.0
77	2711.0
78	2134.5
79	1558.0
80	1242.0
81	926.0
82	926.0
83	755.0
84	584.0
85	584.0
86	433.0
87	282.0
88	216.5
89	151.0
90	151.0
91	112.0
92	73.0
93	70.5
94	68.0
95	68.0
96	56.0
97	44.0
98	44.0
99	167.0
100	290.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12411493883320371
2	0.05329556299955539
3	0.012020466654791026
4	0.0026068481901956437
5	4.3447469836594067E-4
6	1.4482489945531356E-4
7	0.0
8	1.4482489945531356E-4
9	0.0
10	4.3447469836594067E-4
11	0.002317198391285017
12	0.002896497989106271
13	0.01578591404062918
14	0.010717042559693203
15	0.028385680293241455
16	0.012599766252612278
17	0.0256340072035905
18	0.005068871480935974
19	0.007820544570586932
20	0.005213696380391287
21	0.005648171078757229
22	0.008544669067863499
23	0.009703268263506008
24	0.01665486343736106
25	0.020275485923743897
26	0.022592684315028914
27	0.008689493967318813
28	0.005358521279846601
29	0.013323890749888847
30	0.002896497989106271
31	0.005213696380391287
32	0.0066619453749444234
33	0.008255019268952873
34	0.012310116453701652
35	0.017378987934637626
36	0.018972061828646077
37	0.009413618464595382
38	0.005068871480935974
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	690489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.486876284729004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26013407881207	22.75245902057486
2	13.38372800445075	8.428235758092075
3	5.168452412269751	4.882152650659404
4	2.505762169735763	3.1559449374969635
5	1.4247441694121967	2.2430371699835407
6	0.952848094943887	1.800132605018273
7	0.6239522991012761	1.3752416194561876
8	0.47863788495558346	1.2056649495024645
9	0.34850435944554686	0.9875982285495595
>10	2.2735856962970864	14.222997766756615
>50	0.2679908280958862	5.9233806429228775
>100	0.2716682322504458	17.680838914332963
>500	0.024362802553762483	5.1497589070872705
>1k	0.014709616636233951	8.174771868248708
>5k	9.193510397646219E-4	2.0177849613182586
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8446	1.2231911007995784	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5495	0.7958128225069481	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2823	0.40884069116235017	No Hit
CCCTATTAGTGTGTAGTGAACAACGAAGCATGAATGGG	2812	0.40724761726834174	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2794	0.40464076907814606	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCG	2782	0.4029028702846823	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2641	0.3824825594614831	No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGT	2583	0.3740827152930749	No Hit
CTATTAGTGTGTAGTGAACAACGAAGCATGAATGGGTT	2534	0.3669862952197645	No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAA	2440	0.3533727546709651	No Hit
ATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTC	2378	0.34439361090473564	No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTA	1980	0.28675330092152085	No Hit
CACTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTC	1949	0.28226372903840613	No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGAT	1857	0.26893983828851725	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAG	1719	0.24895400216368402	No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTT	1625	0.23534046161488453	No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACA	1617	0.23418186241924202	No Hit
GTACATGGGACACGACCGTACCGCAAACCGACACTGGT	1526	0.2210027965688085	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1524	0.22071314676989787	No Hit
GTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGC	1523	0.22056832187044256	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1447	0.2095616295118387	No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGG	1406	0.20362380863417087	No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCC	1402	0.20304450903634957	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCA	1390	0.20130661024288585	No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGG	1325	0.19189299177829044	No Hit
AGTGTGTAGTGAACAACGAAGCATGAATGGGTTGCAAA	1302	0.18856201909081824	No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCAC	1284	0.1859551709006226	No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTT	1253	0.18146559901750786	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	1171	0.1695899572621722	No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATT	1148	0.16625898457469998	No Hit
GTATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAAC	1108	0.16046598859648742	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1094	0.15843844000411303	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1038	0.15032824563461547	No Hit
CTACACACTAATAGGGCACACCTTCTTCCGAAGTTACG	1005	0.14554902395259012	No Hit
GACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGAT	954	0.13816295408036913	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	949	0.13743882958309256	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	927	0.13425268179507568	No Hit
GTATCAACGCAGAGTACATGGGACACGACCGTACCGCA	912	0.13208030830324596	No Hit
GTAGTGAACAACGAAGCATGAATGGGTTGCAAAAAATC	899	0.13019758461032688	No Hit
CGACCGTACCGCAAACCGACACTGGTGCGCGAGATGAG	891	0.1290389854146844	No Hit
TATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCCG	863	0.1249838882299356	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	853	0.12353563923538245	No Hit
CATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGTGTG	840	0.1216529155424634	No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCAGGAGGAG	832	0.12049431634682088	No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACT	830	0.12020466654791023	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT	775	0.11223929707786799	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	772	0.11180482237950207	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	763	0.11050139828440425	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	746	0.10803937499366391	No Hit
CCTTAGGACCGTTATCGTTACGGCCGCCGTTTACTGGG	746	0.10803937499366391	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	745	0.10789455009420859	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	742	0.10746007539584265	No Hit
AACCGACACTGGTGCGCGAGATGAGTATTCTAAGGCGC	717	0.10383945290945983	No Hit
ACGCAGAGTACATGGGGTCTTTCCGTCTTTCCGCGGGG	711	0.10297050351272795	No Hit
GAGTATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGA	710	0.10282567861327262	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.4482489945531356E-4	0.0
8	0.0	0.0	0.0	1.4482489945531356E-4	0.0
9	0.0	0.0	0.0	2.896497989106271E-4	0.0
10	0.0	0.0	0.0	2.896497989106271E-4	0.0
11	0.0	0.0	0.0	4.3447469836594067E-4	0.0
12	0.0	0.0	0.0	5.792995978212542E-4	0.0
13	0.0	0.0	0.0	8.689493967318813E-4	0.0
14	0.0	0.0	0.0	8.689493967318813E-4	0.0
15	0.0	0.0	0.0	8.689493967318813E-4	0.0
16	0.0	0.0	0.0	0.0010137742961871948	0.0
17	0.0	0.0	0.0	0.0010137742961871948	0.0
18	0.0	0.0	0.0	0.0011585991956425084	0.0
19	0.0	0.0	0.0	0.001303424095097822	0.0
20	0.0	0.0	0.0	0.0018827236929190763	0.0
21	0.0	0.0	0.0	0.0024620232907403305	0.0
22	0.0	0.0	0.0	0.0034757975869275253	0.0
23	0.0	0.0	0.0	0.006082645777123169	0.0
24	0.0	0.0	0.0	0.008255019268952873	0.0
25	0.0	0.0	0.0	0.009992918062416634	5.792995978212542E-4
26	0.0	0.0	0.0	0.012744591152067592	5.792995978212542E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGAAC	20	0.0037559392	31.99399	4
TACCGAG	55	7.5928256E-8	26.180693	7
GCTTAGT	65	3.8135295E-7	22.152893	17
TATACCG	60	4.7178128E-6	21.332417	5
CTAGGAC	45	3.5375517E-4	21.33087	3
TCCTACA	365	0.0	20.613798	2
CCTACAG	320	0.0	20.497635	3
GTCCGTC	55	5.830897E-5	20.36276	7
CTTAATC	55	5.8341488E-5	20.361286	3
CATAGTC	55	5.8374022E-5	20.359814	4
ACCGAGG	80	1.264234E-7	19.99914	8
CTCTCGG	40	0.0044828122	19.99914	15
GTAGACC	40	0.0044846917	19.997692	3
CGGGAGT	40	0.004486572	19.996243	4
TCGGGTA	400	0.0	19.599157	25
ACCGTTA	615	0.0	19.511354	8
GTCCTAT	50	7.145091E-4	19.218662	1
TCTCGAT	50	7.1940373E-4	19.199175	24
CCGACTA	50	7.1940373E-4	19.199175	9
AAGCTTA	100	3.25781E-9	19.199175	15
>>END_MODULE