FastQCFastQC Report
Thu 2 Feb 2017
SRR4063049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences690489
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT99161.436083702998889No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT65820.9532374882148738No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT39810.5765479247316032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31090.4502606124065698No Hit
CCCTATTAGTGTGTAGTGAACAACGAAGCATGAATGGG28870.4181094847274902No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGT27770.4021787457874057No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA26600.38523423255113404No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCG26530.38422045825494683No Hit
CTATTAGTGTGTAGTGAACAACGAAGCATGAATGGGTT26230.37987571127128744No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAA25990.3763999136843599No Hit
ATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTC24720.3580071514535351No Hit
CACTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTC21260.3078977362419966No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTA20470.29645656918502683No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACA18360.2658985153999557No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA16370.2370783604083483No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGG15650.2266509676475657No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAG15570.2254923684519232No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGG14900.21578910018841718No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGAT14870.21535462549005127No Hit
GTACATGGGACACGACCGTACCGCAAACCGACACTGGT14300.20709960622109835No Hit
GTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGC14230.2060858319249112No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCAC14080.20391345843308148No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCC13600.19696186325922643No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTT13570.19652738856086047No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA13320.19290676607447765No Hit
AGTGTGTAGTGAACAACGAAGCATGAATGGGTTGCAAA13270.19218264157720108No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA13210.1913136921804692No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTT13180.19087921748210326No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT12850.1860999958000779No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCA12270.17770015163166974No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.1697347821616275No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATT11450.16582450987633401No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA10730.15539711711555143No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA10210.14786622234387514No Hit
CATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGTGTG10120.14656279824877733No Hit
GTATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAAC9800.14192840146620728No Hit
CTACACACTAATAGGGCACACCTTCTTCCGAAGTTACG9630.13946637817546695No Hit
CGACCGTACCGCAAACCGACACTGGTGCGCGAGATGAG9410.13628023038745007No Hit
GTAGTGAACAACGAAGCATGAATGGGTTGCAAAAAATC9350.13541128099071817No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCAGGAGGAG9320.13497680629235223No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.1348319813928969No Hit
TATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCCG9110.13193548340379063No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA9020.13063205930869282No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT8600.12454941353156966No Hit
GACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGAT8550.12382528903429309No Hit
GTATCAACGCAGAGTACATGGGACACGACCGTACCGCA8350.12092879104518683No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8140.11788746815662524No Hit
CCTTAGGACCGTTATCGTTACGGCCGCCGTTTACTGGG8060.11672886896098272No Hit
ACGCAGAGTACATGGGGTCTTTCCGTCTTTCCGCGGGG7720.11180482237950207No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACT7720.11180482237950207No Hit
AACCGACACTGGTGCGCGAGATGAGTATTCTAAGGCGC7540.10919797418930642No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA7480.10832902479257454No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC7360.10659112599911078No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT7340.10630147620020015No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC7230.10470840230619169No Hit
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGG7010.10152225451817479No Hit
GCATGAATGGGTTGCAAAAAATCGGTGGCTGCGACTGT7000.10137742961871948No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA6950.10065330512144292No Hit
GAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG6920.10021883042307697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA4450.024.80820525
GGTAAGT4700.024.16944528
GGGTAAG4900.023.1829427
AAGTTCC4900.023.1829431
TATGCGC350.002064351722.8597310
TCGGATT350.00206609122.85641728
GTAAGTT5050.022.81115729
AAATGTC854.292815E-1022.590797
GCCAGAT502.7962033E-522.3992926
ATGTCCA1001.4370016E-1020.8023559
TAAGTTC5700.020.77127330
AGTTCCG5800.020.68900132
GATATAC1800.020.4437961
TGTCGGG5450.020.25623923
TTGAATA400.00448064220.00081411
ATTTAGA801.2640885E-719.9993671
AATGTCC1052.7830538E-1019.8117668
TAGAAAT902.033812E-819.556354
GTCGGGT5700.019.36780724
TAGCGGG507.1934913E-419.19939212
Produced by FastQC (version 0.11.5)