##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063049_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690489
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.893912864650993	32.0	32.0	32.0	32.0	32.0
2	31.28649551260049	32.0	32.0	32.0	32.0	32.0
3	31.359613259588496	32.0	32.0	32.0	32.0	32.0
4	31.480594187597486	32.0	32.0	32.0	32.0	32.0
5	31.37933841089431	32.0	32.0	32.0	32.0	32.0
6	34.84497508287605	36.0	36.0	36.0	32.0	36.0
7	34.921005258592096	36.0	36.0	36.0	36.0	36.0
8	34.830830034946246	36.0	36.0	36.0	32.0	36.0
9	34.95212233648907	36.0	36.0	36.0	36.0	36.0
10	34.82150765616831	36.0	36.0	36.0	32.0	36.0
11	34.99267041183857	36.0	36.0	36.0	36.0	36.0
12	34.90489493677669	36.0	36.0	36.0	32.0	36.0
13	34.95542724069464	36.0	36.0	36.0	36.0	36.0
14	34.861713944755095	36.0	36.0	36.0	32.0	36.0
15	34.865305602261586	36.0	36.0	36.0	32.0	36.0
16	34.88393298082953	36.0	36.0	36.0	32.0	36.0
17	34.84048985573992	36.0	36.0	36.0	32.0	36.0
18	34.8392443616046	36.0	36.0	36.0	32.0	36.0
19	34.78798648494038	36.0	36.0	36.0	32.0	36.0
20	34.75003512003812	36.0	36.0	36.0	32.0	36.0
21	34.73176690722082	36.0	36.0	36.0	32.0	36.0
22	34.718303984567456	36.0	36.0	36.0	32.0	36.0
23	34.72243294245093	36.0	36.0	36.0	32.0	36.0
24	34.683425804031636	36.0	36.0	36.0	32.0	36.0
25	34.68616009813335	36.0	36.0	36.0	32.0	36.0
26	34.63231709701385	36.0	36.0	36.0	32.0	36.0
27	34.618334252971444	36.0	36.0	36.0	32.0	36.0
28	34.59868006586636	36.0	36.0	36.0	32.0	36.0
29	34.5520073455189	36.0	36.0	36.0	32.0	36.0
30	34.5132420646817	36.0	36.0	36.0	32.0	36.0
31	34.48698965515743	36.0	36.0	36.0	32.0	36.0
32	34.459477269007905	36.0	36.0	36.0	32.0	36.0
33	34.376494049869	36.0	36.0	36.0	32.0	36.0
34	34.331492608861254	36.0	36.0	36.0	32.0	36.0
35	34.280004460606904	36.0	36.0	36.0	32.0	36.0
36	34.21649439744876	36.0	36.0	36.0	32.0	36.0
37	34.20782952371435	36.0	36.0	36.0	32.0	36.0
38	33.86311874627981	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	0.0
20	8.0
21	19.0
22	49.0
23	163.0
24	434.0
25	1212.0
26	2661.0
27	4993.0
28	8927.0
29	13978.0
30	20922.0
31	30133.0
32	41807.0
33	64613.0
34	151972.0
35	348596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.783419295874815	19.94635126307724	12.665244337842202	24.604985103205742
2	15.13593989187373	21.223509715578377	39.35167685509835	24.288873537449547
3	18.612461603298534	28.79350721010762	28.79698300769455	23.797048178899303
4	11.101729648784119	16.836088152275135	38.08268656759649	33.97949563134425
5	11.816987671056308	40.20136454020267	32.628180897885414	15.35346689085561
6	28.532718888670043	39.12580667585823	18.11950388710593	14.221970548365793
7	27.66390385832485	33.522718221066185	20.397664816265763	18.415713104343205
8	28.72635320084464	32.65640704791201	18.49323797854807	20.12400177269528
9	30.687914214209577	13.937439171339852	18.977672985123046	36.39697362932752
10	17.595705023020184	25.35420960171243	30.80664058822763	26.24344478703976
11	35.4435751341863	24.212768915705226	21.82884404264995	18.51481190745853
12	24.46621008071115	25.919821806927573	29.143923057204553	20.47004505515672
13	28.815272676715598	22.50567714428057	27.28635399891092	21.392696180092923
14	23.305946944846333	22.493189609103116	26.23560983592787	27.96525361012268
15	24.328265910101393	28.96526954086162	25.967249297237178	20.739215251799813
16	23.568224837759907	27.810290967705498	25.85066525317565	22.77081894135895
17	21.8495877559237	26.171162755670256	26.713242354331495	25.26600713407455
18	21.533579825312206	25.304675382229114	30.22654959021795	22.93519520224073
19	23.753166234364343	25.88325085555309	30.111993094748797	20.25158981533377
20	24.26381882984378	24.85108379713507	28.917477324041368	21.96762004897978
21	27.084033321361122	22.7434799735839	27.161659579891325	23.01082712516365
22	24.624288004588053	24.125655875763407	28.4318794361677	22.818176683480836
23	23.64747830519864	25.07530905678303	29.859316888924937	21.417895749093393
24	22.39499832727241	26.183762521922873	28.038969483945436	23.382269666859283
25	23.96374163817237	25.957111554275304	28.148457107933655	21.93068969961868
26	22.59587046281693	25.53291942377069	29.92849994713891	21.942710166273464
27	23.24541013687401	26.444736990741347	28.746004643086277	21.563848229298365
28	22.59934626040386	25.735529458108676	28.848251022101728	22.81687325938574
29	22.427004630052036	25.34218503118804	29.415385328368735	22.815425010391184
30	22.795728824065264	25.584766737775695	29.6964904582115	21.923013979947545
31	22.044087595892186	27.29254195215275	29.33399373487485	21.329376717080216
32	21.98586798631115	27.250687556210163	28.04736932811384	22.71607512936484
33	22.49058276091292	26.961617056897357	28.250413837150194	22.29738634503953
34	23.029910686484506	26.133363456912416	29.341814279445437	21.494911577157637
35	22.63352493667531	26.202444933952606	30.317789276874795	20.84624085249729
36	23.394000483715164	26.04878571563052	28.34512932139397	22.212084479260348
37	23.389510911832048	27.21853642853109	27.39971237774968	21.992240281887184
38	22.839610768600224	27.493848562395634	27.988425594035533	21.67811507496861
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	86.0
1	107.0
2	128.0
3	128.0
4	588.5
5	1049.0
6	1049.0
7	1676.0
8	2303.0
9	2689.0
10	3075.0
11	3075.0
12	3807.5
13	4540.0
14	5361.0
15	6182.0
16	6182.0
17	8834.0
18	11486.0
19	11486.0
20	12605.0
21	13724.0
22	11971.0
23	10218.0
24	10218.0
25	10200.5
26	10183.0
27	10183.0
28	13413.0
29	16643.0
30	20346.0
31	24049.0
32	24049.0
33	25769.0
34	27489.0
35	27489.0
36	28700.0
37	29911.0
38	36031.0
39	42151.0
40	42151.0
41	44359.0
42	46567.0
43	52762.5
44	58958.0
45	58958.0
46	61768.5
47	64579.0
48	64579.0
49	67534.5
50	70490.0
51	60891.5
52	51293.0
53	51293.0
54	52075.0
55	52857.0
56	52857.0
57	49424.5
58	45992.0
59	36440.5
60	26889.0
61	26889.0
62	25655.0
63	24421.0
64	20407.0
65	16393.0
66	16393.0
67	13921.5
68	11450.0
69	11450.0
70	9399.5
71	7349.0
72	5761.5
73	4174.0
74	4174.0
75	3384.5
76	2595.0
77	2595.0
78	2041.5
79	1488.0
80	1165.5
81	843.0
82	843.0
83	676.5
84	510.0
85	510.0
86	387.5
87	265.0
88	181.0
89	97.0
90	97.0
91	64.5
92	32.0
93	26.5
94	21.0
95	21.0
96	13.0
97	5.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010427392760782575
2	0.0
3	0.0
4	2.896497989106271E-4
5	0.0
6	1.4482489945531356E-4
7	0.0011585991956425084
8	0.0021723734918297033
9	0.003620622486382839
10	0.0011585991956425084
11	0.004489571883114721
12	2.896497989106271E-4
13	1.4482489945531356E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.4482489945531356E-4
22	0.0
23	1.4482489945531356E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	690489.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.641555453443573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.09627250269739	19.790247205122554
2	14.910566142432275	8.541236140214243
3	5.705784438194557	4.902676241758344
4	2.824356373103488	3.235758387221213
5	1.6153123786147954	2.3132529533364754
6	1.0066530468403678	1.7299265438073796
7	0.6926223838875964	1.3886447691489012
8	0.5159646980508579	1.1822425208994334
9	0.4122950508471137	1.062789440581615
>10	2.5486844249783798	14.520370429349311
>50	0.3220714361876642	6.4634756057245015
>100	0.30181537101837747	18.08171913611629
>500	0.029877696124302463	5.73333768414436
>1k	0.016711253764440364	8.661431518228358
>5k	0.001012803258450931	2.392891424347028
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9916	1.436083702998889	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6582	0.9532374882148738	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3981	0.5765479247316032	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3109	0.4502606124065698	No Hit
CCCTATTAGTGTGTAGTGAACAACGAAGCATGAATGGG	2887	0.4181094847274902	No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGT	2777	0.4021787457874057	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2660	0.38523423255113404	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCG	2653	0.38422045825494683	No Hit
CTATTAGTGTGTAGTGAACAACGAAGCATGAATGGGTT	2623	0.37987571127128744	No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAA	2599	0.3763999136843599	No Hit
ATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTC	2472	0.3580071514535351	No Hit
CACTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTC	2126	0.3078977362419966	No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTA	2047	0.29645656918502683	No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACA	1836	0.2658985153999557	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1637	0.2370783604083483	No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGG	1565	0.2266509676475657	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAG	1557	0.2254923684519232	No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGG	1490	0.21578910018841718	No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGAT	1487	0.21535462549005127	No Hit
GTACATGGGACACGACCGTACCGCAAACCGACACTGGT	1430	0.20709960622109835	No Hit
GTACATGGGGTCTTTCCGTCTTTCCGCGGGGAGATTGC	1423	0.2060858319249112	No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCAC	1408	0.20391345843308148	No Hit
GTATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCC	1360	0.19696186325922643	No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTT	1357	0.19652738856086047	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1332	0.19290676607447765	No Hit
AGTGTGTAGTGAACAACGAAGCATGAATGGGTTGCAAA	1327	0.19218264157720108	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1321	0.1913136921804692	No Hit
CTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTT	1318	0.19087921748210326	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1285	0.1860999958000779	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCA	1227	0.17770015163166974	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1172	0.1697347821616275	No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATT	1145	0.16582450987633401	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	1073	0.15539711711555143	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	1021	0.14786622234387514	No Hit
CATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGTGTG	1012	0.14656279824877733	No Hit
GTATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAAC	980	0.14192840146620728	No Hit
CTACACACTAATAGGGCACACCTTCTTCCGAAGTTACG	963	0.13946637817546695	No Hit
CGACCGTACCGCAAACCGACACTGGTGCGCGAGATGAG	941	0.13628023038745007	No Hit
GTAGTGAACAACGAAGCATGAATGGGTTGCAAAAAATC	935	0.13541128099071817	No Hit
ATCACAAACATTTCAACTTCGCTGAGTCTCAGGAGGAG	932	0.13497680629235223	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	931	0.1348319813928969	No Hit
TATCAACGCAGAGTACATGGGGTCTTTCCGTCTTTCCG	911	0.13193548340379063	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	902	0.13063205930869282	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	860	0.12454941353156966	No Hit
GACGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGAT	855	0.12382528903429309	No Hit
GTATCAACGCAGAGTACATGGGACACGACCGTACCGCA	835	0.12092879104518683	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	814	0.11788746815662524	No Hit
CCTTAGGACCGTTATCGTTACGGCCGCCGTTTACTGGG	806	0.11672886896098272	No Hit
ACGCAGAGTACATGGGGTCTTTCCGTCTTTCCGCGGGG	772	0.11180482237950207	No Hit
GTGTGGCCATCGTTACGCCATTCGTGCAGGTCGGAACT	772	0.11180482237950207	No Hit
AACCGACACTGGTGCGCGAGATGAGTATTCTAAGGCGC	754	0.10919797418930642	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	748	0.10832902479257454	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	736	0.10659112599911078	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT	734	0.10630147620020015	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	723	0.10470840230619169	No Hit
CCTTAGGACCGTTATAGTTACGGCCGCCGTTTACTGGG	701	0.10152225451817479	No Hit
GCATGAATGGGTTGCAAAAAATCGGTGGCTGCGACTGT	700	0.10137742961871948	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	695	0.10065330512144292	No Hit
GAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG	692	0.10021883042307697	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.4482489945531356E-4
6	0.0	0.0	0.0	0.0	1.4482489945531356E-4
7	0.0	0.0	0.0	0.0	1.4482489945531356E-4
8	0.0	0.0	0.0	0.0	1.4482489945531356E-4
9	0.0	0.0	0.0	0.0	2.896497989106271E-4
10	0.0	0.0	0.0	0.0	2.896497989106271E-4
11	0.0	0.0	0.0	1.4482489945531356E-4	2.896497989106271E-4
12	0.0	0.0	0.0	2.896497989106271E-4	2.896497989106271E-4
13	0.0	0.0	0.0	5.792995978212542E-4	2.896497989106271E-4
14	0.0	0.0	0.0	5.792995978212542E-4	2.896497989106271E-4
15	0.0	0.0	0.0	5.792995978212542E-4	2.896497989106271E-4
16	0.0	0.0	0.0	7.241244972765677E-4	2.896497989106271E-4
17	0.0	0.0	0.0	7.241244972765677E-4	2.896497989106271E-4
18	0.0	0.0	0.0	8.689493967318813E-4	2.896497989106271E-4
19	0.0	0.0	0.0	0.0010137742961871948	2.896497989106271E-4
20	0.0	0.0	0.0	0.001303424095097822	2.896497989106271E-4
21	0.0	0.0	0.0	0.0020275485923743897	2.896497989106271E-4
22	0.0	0.0	0.0	0.003186147788016898	2.896497989106271E-4
23	0.0	0.0	0.0	0.005792995978212542	2.896497989106271E-4
24	0.0	0.0	0.0	0.007965369470042245	2.896497989106271E-4
25	0.0	0.0	0.0	0.009703268263506008	4.3447469836594067E-4
26	0.0	0.0	0.0	0.012744591152067592	4.3447469836594067E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGTA	445	0.0	24.808205	25
GGTAAGT	470	0.0	24.169445	28
GGGTAAG	490	0.0	23.18294	27
AAGTTCC	490	0.0	23.18294	31
TATGCGC	35	0.0020643517	22.85973	10
TCGGATT	35	0.002066091	22.856417	28
GTAAGTT	505	0.0	22.811157	29
AAATGTC	85	4.292815E-10	22.59079	7
GCCAGAT	50	2.7962033E-5	22.39929	26
ATGTCCA	100	1.4370016E-10	20.802355	9
TAAGTTC	570	0.0	20.771273	30
AGTTCCG	580	0.0	20.689001	32
GATATAC	180	0.0	20.443796	1
TGTCGGG	545	0.0	20.256239	23
TTGAATA	40	0.004480642	20.000814	11
ATTTAGA	80	1.2640885E-7	19.999367	1
AATGTCC	105	2.7830538E-10	19.811766	8
TAGAAAT	90	2.033812E-8	19.55635	4
GTCGGGT	570	0.0	19.367807	24
TAGCGGG	50	7.1934913E-4	19.199392	12
>>END_MODULE