FastQCFastQC Report
Thu 2 Feb 2017
SRR4063048_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063048_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569080
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30770.19610217452265022No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24910.15875544905294822No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23730.15123511866826422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20370.12982129655594363No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20020.12759069008591023No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19500.12427664618757488No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18650.11885945904606521No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18510.11796721645805186No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18350.11694751064317944No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18300.11662885257603182No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17960.11446197771942794No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17330.11044688607336783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA507.1968185E-419.2002859
CTAGCGG4900.017.63179229
TCTAGCG4950.017.45369328
TAGCGGC5300.016.30109230
CGCCGGT5400.016.2949817
GTATCAA26150.016.2340551
ATACCGT3750.016.2094426
ACCGTCG3900.015.9987118
CGCAAGA5450.015.8620422
CGGTCCA5650.015.85864410
TACCGTC3750.015.7853947
GGCATCG4000.015.59874226
GTCCTAT5550.015.58667851
CAAGACG5750.015.5793664
CCTATTC5350.015.5511393
TACGCTA3200.015.5002329
GCGCAAG5700.015.4575491
ACGCTAT3350.015.2838110
ATCGTTT4100.015.21876929
GTCTTAG2450.015.0388481
Produced by FastQC (version 0.11.5)