##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063048_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569080
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289310296479467	32.0	32.0	32.0	32.0	32.0
2	30.920853621230275	32.0	32.0	32.0	32.0	32.0
3	30.97315560710735	32.0	32.0	32.0	32.0	32.0
4	31.03644556045581	32.0	32.0	32.0	32.0	32.0
5	30.954570831315166	32.0	32.0	32.0	32.0	32.0
6	34.62291215234405	36.0	36.0	36.0	32.0	36.0
7	34.55828192316517	36.0	36.0	36.0	32.0	36.0
8	34.540427511662884	36.0	36.0	36.0	32.0	36.0
9	34.676341550462695	36.0	36.0	36.0	32.0	36.0
10	34.40917990159839	36.0	36.0	36.0	32.0	36.0
11	34.64288181609606	36.0	36.0	36.0	32.0	36.0
12	34.474565987712545	36.0	36.0	36.0	32.0	36.0
13	34.5625213500905	36.0	36.0	36.0	32.0	36.0
14	34.45899826650012	36.0	36.0	36.0	32.0	36.0
15	34.41153159813394	36.0	36.0	36.0	32.0	36.0
16	34.42257883600581	36.0	36.0	36.0	32.0	36.0
17	34.35235488311622	36.0	36.0	36.0	32.0	36.0
18	34.38397532311928	36.0	36.0	36.0	32.0	36.0
19	34.36655747316899	36.0	36.0	36.0	32.0	36.0
20	34.35238547429067	36.0	36.0	36.0	32.0	36.0
21	34.3450531521656	36.0	36.0	36.0	32.0	36.0
22	34.317941086496546	36.0	36.0	36.0	32.0	36.0
23	34.27103270706401	36.0	36.0	36.0	32.0	36.0
24	34.24803451704183	36.0	36.0	36.0	32.0	36.0
25	34.22716177632753	36.0	36.0	36.0	32.0	36.0
26	34.18459351976955	36.0	36.0	36.0	32.0	36.0
27	34.18694967751804	36.0	36.0	36.0	32.0	36.0
28	34.17434483901395	36.0	36.0	36.0	32.0	36.0
29	34.143110612588266	36.0	36.0	36.0	32.0	36.0
30	34.11828332526066	36.0	36.0	36.0	32.0	36.0
31	34.13772337930507	36.0	36.0	36.0	32.0	36.0
32	34.092314604736536	36.0	36.0	36.0	32.0	36.0
33	34.06147232773345	36.0	36.0	36.0	32.0	36.0
34	34.071371759247455	36.0	36.0	36.0	32.0	36.0
35	34.03020559818492	36.0	36.0	36.0	32.0	36.0
36	33.9922228312132	36.0	36.0	36.0	32.0	36.0
37	33.993458587197594	36.0	36.0	36.0	32.0	36.0
38	33.415632090142	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	5.0
9	4.0
10	15.0
11	12.0
12	7.0
13	12.0
14	128.0
15	315.0
16	437.0
17	539.0
18	642.0
19	871.0
20	1183.0
21	1768.0
22	2595.0
23	3904.0
24	6051.0
25	9528.0
26	14190.0
27	20087.0
28	28633.0
29	38513.0
30	52287.0
31	71074.0
32	97617.0
33	147876.0
34	337560.0
35	733224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.63823698132856	17.024885253705285	11.241850668058575	26.095027096907575
2	17.15326954409078	19.09372937504185	36.98447568367252	26.768525397194846
3	19.239560254648456	22.816570506364936	28.2539192865356	29.689949952451006
4	12.693135980859596	14.729926700262643	35.680165604762664	36.89677171411509
5	14.945483838896267	36.07078305958411	33.51972753391168	15.464005567607941
6	35.26837752385638	35.0356929583443	16.29569485755001	13.400234660249305
7	31.1019195961965	30.046524077803554	20.29424885920412	18.55730746679583
8	28.60744617526006	32.6413540725294	19.065576257079016	19.685623495131523
9	27.328585313002883	14.159042748115928	18.514921211541832	39.997450727339356
10	15.997618973265071	26.273317141161872	31.261300476524006	26.46776340904905
11	38.31601716703186	20.962885917246954	21.560379693751777	19.160717221969417
12	24.817339608115066	23.51813092403469	28.26851205074211	23.396017417108126
13	29.501685652164767	18.88185391779474	25.15991226577275	26.456548164267744
14	23.56714489586102	19.538686976269556	24.657007749951717	32.237160377917704
15	25.41868297263476	26.926649280377184	21.966648158406905	25.688019588581145
16	26.25578037905901	25.663193924347542	23.24319500791964	24.837830688673805
17	24.315146647287932	25.873796000586463	24.774690354618066	25.036366997507535
18	25.456458682418713	24.4883781256752	25.676219151156072	24.378944040750014
19	25.57382691498719	24.948245312121557	25.208484709421647	24.269443063469602
20	25.905282714621237	24.130823430434326	24.687552780937995	25.276341074006442
21	27.19721141184704	24.04290469009526	24.194915632333654	24.564968265724048
22	26.020933665443774	23.972820142821085	24.946142530447	25.060103661288142
23	24.494776628359816	23.845472334295785	25.516250135444352	26.143500901900047
24	24.862429589382682	24.83323591484881	25.02669181487131	25.2776426808972
25	25.0397915857927	24.34155044718922	25.08842751684569	25.530230450172393
26	24.794742867633374	25.403693608471382	25.324395511370735	24.47716801252451
27	25.62960347346334	24.526618242821193	24.598387174904584	25.245391108810878
28	24.68568791738847	24.47351212442208	25.57052917114409	25.270270787045355
29	24.473852111953384	24.944435698064186	25.572199173562677	25.009513016419753
30	24.545564051234102	24.979398138853675	25.786583201245605	24.688454608666614
31	25.21197094900141	24.883155455985314	24.478047630125594	25.426825964887676
32	24.946413377056704	24.63340248764305	24.436392375817025	25.98379175948322
33	24.486655856737848	24.31258795814722	25.180314201798936	26.020441983315994
34	25.44576897139455	24.589543248545763	25.16792426612411	24.79676351393558
35	26.0894923776329	24.240567974074516	25.27543275004398	24.3945068982486
36	24.597085707729377	25.222332708660012	24.757715990362183	25.422865593248428
37	25.748178194939037	24.856413142619306	24.78311389237503	24.612294770066626
38	24.672189189206414	24.493154421789086	25.318983357861118	25.515673031143383
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	90.5
2	130.0
3	130.0
4	336.0
5	542.0
6	542.0
7	704.5
8	867.0
9	885.5
10	904.0
11	904.0
12	1203.0
13	1502.0
14	2125.0
15	2748.0
16	2748.0
17	4079.5
18	5411.0
19	5411.0
20	6797.0
21	8183.0
22	9159.0
23	10135.0
24	10135.0
25	12202.5
26	14270.0
27	14270.0
28	18337.0
29	22404.0
30	27231.0
31	32058.0
32	32058.0
33	41317.0
34	50576.0
35	50576.0
36	57917.5
37	65259.0
38	74631.0
39	84003.0
40	84003.0
41	90452.5
42	96902.0
43	110052.5
44	123203.0
45	123203.0
46	126517.5
47	129832.0
48	129832.0
49	137914.0
50	145996.0
51	149054.5
52	152113.0
53	152113.0
54	144308.5
55	136504.0
56	136504.0
57	132454.5
58	128405.0
59	115084.0
60	101763.0
61	101763.0
62	96010.5
63	90258.0
64	75252.5
65	60247.0
66	60247.0
67	50589.0
68	40931.0
69	40931.0
70	32699.0
71	24467.0
72	19257.5
73	14048.0
74	14048.0
75	10712.0
76	7376.0
77	7376.0
78	6945.5
79	6515.0
80	5144.5
81	3774.0
82	3774.0
83	3803.5
84	3833.0
85	3833.0
86	2547.0
87	1261.0
88	1099.5
89	938.0
90	938.0
91	663.5
92	389.0
93	342.0
94	295.0
95	295.0
96	273.5
97	252.0
98	252.0
99	493.5
100	735.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1228108190786958
2	0.0570397940194254
3	0.0109618375098784
4	0.0027404593774696
5	2.549264537181023E-4
6	1.9119484028857676E-4
7	0.0
8	3.823896805771535E-4
9	3.186580671476279E-4
10	5.735845208657302E-4
11	0.001657021949167665
12	0.003059117444617228
13	0.01599663497081092
14	0.010643179442730771
15	0.026193693119535014
16	0.012427664618757488
17	0.022306064700333954
18	0.006309429729523032
19	0.008922425880133582
20	0.00605450327580493
21	0.0072654039309659165
22	0.007902720065261173
23	0.010834374283019348
24	0.01574170851709282
25	0.01790858337369669
26	0.020139189843730084
27	0.00994213169500599
28	0.006245698116093506
29	0.01128049557702603
30	0.002995385831187702
31	0.007329135544395442
32	0.007966451678690697
33	0.00968720524128789
34	0.013893491727636577
35	0.01758992530654906
36	0.01657021949167665
37	0.010770642669589823
38	0.0070742090906773395
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1569080.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.816447125460535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.40432276738895	47.06508723064666
2	11.927417648329227	13.792018236158231
3	3.1943094762645625	5.540508748104229
4	1.210996074661438	2.800619620792132
5	0.6268267106737896	1.8120446687248752
6	0.36086887130781237	1.2518493610315655
7	0.2245501706916517	0.9087885149564913
8	0.17443888973788535	0.8068349476123591
9	0.12745068229623496	0.6631871070675726
>10	0.6052886504534031	6.355013701189123
>50	0.05995495810598701	2.472154314797036
>100	0.06894409215888846	8.767246256127866
>500	0.010086528194277064	3.8893408783773316
>1k	0.004544479735883073	3.875306414414559
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3077	0.19610217452265022	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2491	0.15875544905294822	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2373	0.15123511866826422	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2037	0.12982129655594363	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2002	0.12759069008591023	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1950	0.12427664618757488	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1865	0.11885945904606521	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1851	0.11796721645805186	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1835	0.11694751064317944	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1830	0.11662885257603182	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1796	0.11446197771942794	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1733	0.11044688607336783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.373161342952558E-5
2	0.0	0.0	0.0	0.0	6.373161342952558E-5
3	0.0	0.0	0.0	0.0	6.373161342952558E-5
4	0.0	0.0	0.0	0.0	6.373161342952558E-5
5	0.0	0.0	0.0	0.0	6.373161342952558E-5
6	0.0	0.0	0.0	0.0	6.373161342952558E-5
7	0.0	0.0	0.0	0.0	1.2746322685905115E-4
8	0.0	0.0	0.0	6.373161342952558E-5	1.2746322685905115E-4
9	0.0	0.0	0.0	6.373161342952558E-5	1.2746322685905115E-4
10	0.0	0.0	0.0	6.373161342952558E-5	1.2746322685905115E-4
11	0.0	0.0	0.0	6.373161342952558E-5	1.2746322685905115E-4
12	0.0	0.0	0.0	6.373161342952558E-5	3.823896805771535E-4
13	0.0	0.0	0.0	6.373161342952558E-5	5.098529074362046E-4
14	0.0	0.0	0.0	6.373161342952558E-5	7.64779361154307E-4
15	0.0	0.0	0.0	1.2746322685905115E-4	9.559742014428837E-4
16	0.0	0.0	0.0	1.9119484028857676E-4	0.0010197058148724092
17	0.0	0.0	0.0	1.9119484028857676E-4	0.001083437428301935
18	0.0	0.0	0.0	1.9119484028857676E-4	0.001083437428301935
19	0.0	0.0	0.0	2.549264537181023E-4	0.001083437428301935
20	0.0	0.0	0.0	2.549264537181023E-4	0.0011471690417314605
21	0.0	0.0	0.0	3.823896805771535E-4	0.001210900655160986
22	0.0	0.0	0.0	5.098529074362046E-4	0.0014020954954495628
23	0.0	0.0	0.0	6.373161342952558E-4	0.001529558722308614
24	0.0	0.0	0.0	7.010477477247814E-4	0.001529558722308614
25	0.0	0.0	0.0	0.001083437428301935	0.001529558722308614
26	0.0	0.0	0.0	0.001210900655160986	0.0015932903357381396
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATGTA	50	7.1968185E-4	19.200285	9
CTAGCGG	490	0.0	17.631792	29
TCTAGCG	495	0.0	17.453693	28
TAGCGGC	530	0.0	16.301092	30
CGCCGGT	540	0.0	16.294981	7
GTATCAA	2615	0.0	16.234055	1
ATACCGT	375	0.0	16.209442	6
ACCGTCG	390	0.0	15.998711	8
CGCAAGA	545	0.0	15.862042	2
CGGTCCA	565	0.0	15.858644	10
TACCGTC	375	0.0	15.785394	7
GGCATCG	400	0.0	15.598742	26
GTCCTAT	555	0.0	15.5866785	1
CAAGACG	575	0.0	15.579366	4
CCTATTC	535	0.0	15.551139	3
TACGCTA	320	0.0	15.500232	9
GCGCAAG	570	0.0	15.457549	1
ACGCTAT	335	0.0	15.28381	10
ATCGTTT	410	0.0	15.218769	29
GTCTTAG	245	0.0	15.038848	1
>>END_MODULE