FastQCFastQC Report
Thu 2 Feb 2017
SRR4063048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1569080
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31440.20037219262242842No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26120.16646697427792082No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23780.15155377673541184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22370.14256761924184871No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21090.13440997272286945No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20780.13243429270655416No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19890.1267621791113264No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19680.12542381522930635No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17900.1140795880388508No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17410.11095673898080403No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17180.10949091187192495No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17050.10866240089734112No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16870.10751523185560967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3800.017.68357728
CTAGCGG3850.017.4539229
CATCGTT2950.017.3553128
GTATCAA25900.016.8669471
ATCGTTT3150.016.25338629
ATTGGAC908.577339E-616.0014653
TAGCGGC4350.015.447721530
AGGCCCG5900.014.91519510
GCGCAAG5150.014.9144641
TACCGTC4300.014.8850857
ACGGTAT5950.014.7912719
GTTCAAA6600.014.7897041
CGCAAGA5350.014.6541472
ACCGTCG4550.014.4189038
CAAGACG5550.014.4157354
AGGGGCG4000.014.39948432
AGCGGCG4900.014.36683231
CGACGGT5950.014.2534077
GACGGTA5950.014.2534078
CGACAAT901.5352166E-414.22171117
Produced by FastQC (version 0.11.5)