##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063048_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1569080
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243048155607106	32.0	32.0	32.0	32.0	32.0
2	31.318798276697173	32.0	32.0	32.0	32.0	32.0
3	31.396401075789633	32.0	32.0	32.0	32.0	32.0
4	31.487778825808753	32.0	32.0	32.0	32.0	32.0
5	31.417176307135392	32.0	32.0	32.0	32.0	32.0
6	34.95526295663701	36.0	36.0	36.0	36.0	36.0
7	34.98789545466133	36.0	36.0	36.0	36.0	36.0
8	34.92099191883142	36.0	36.0	36.0	36.0	36.0
9	35.031002243352795	36.0	36.0	36.0	36.0	36.0
10	34.879825757768884	36.0	36.0	36.0	32.0	36.0
11	35.050246641343975	36.0	36.0	36.0	36.0	36.0
12	34.951728401356206	36.0	36.0	36.0	36.0	36.0
13	35.00388890305147	36.0	36.0	36.0	36.0	36.0
14	34.951305223443036	36.0	36.0	36.0	32.0	36.0
15	34.92310844571341	36.0	36.0	36.0	32.0	36.0
16	34.93459160782114	36.0	36.0	36.0	36.0	36.0
17	34.898680118285874	36.0	36.0	36.0	32.0	36.0
18	34.8981454100492	36.0	36.0	36.0	32.0	36.0
19	34.89720791801565	36.0	36.0	36.0	32.0	36.0
20	34.88369490402019	36.0	36.0	36.0	32.0	36.0
21	34.885195146200324	36.0	36.0	36.0	32.0	36.0
22	34.863673617661306	36.0	36.0	36.0	32.0	36.0
23	34.80664147143549	36.0	36.0	36.0	32.0	36.0
24	34.783698090600865	36.0	36.0	36.0	32.0	36.0
25	34.767770285772556	36.0	36.0	36.0	32.0	36.0
26	34.706419685420755	36.0	36.0	36.0	32.0	36.0
27	34.693717337548115	36.0	36.0	36.0	32.0	36.0
28	34.66596158258343	36.0	36.0	36.0	32.0	36.0
29	34.63114245290234	36.0	36.0	36.0	32.0	36.0
30	34.60803464450506	36.0	36.0	36.0	32.0	36.0
31	34.60996953628878	36.0	36.0	36.0	32.0	36.0
32	34.58770553445331	36.0	36.0	36.0	32.0	36.0
33	34.55479516659444	36.0	36.0	36.0	32.0	36.0
34	34.54901789583705	36.0	36.0	36.0	32.0	36.0
35	34.51596604379637	36.0	36.0	36.0	32.0	36.0
36	34.49956662502868	36.0	36.0	36.0	32.0	36.0
37	34.49931297320723	36.0	36.0	36.0	32.0	36.0
38	34.07165281566268	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	26.0
22	83.0
23	308.0
24	961.0
25	2404.0
26	4923.0
27	9527.0
28	17116.0
29	27258.0
30	41178.0
31	60076.0
32	86830.0
33	140963.0
34	351239.0
35	826183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.038541063811884	18.27794080529076	11.908352475964419	26.775165654932938
2	16.158768195375632	20.088204552986465	36.32370561093124	27.429321640706654
3	18.24954750554465	23.545580849924796	28.56419048104622	29.640681163484334
4	12.084939107513526	15.516255735059529	35.699012859152226	36.69979229827472
5	14.362756750934786	36.737538390355105	33.191445427540614	15.708259431169495
6	35.3402218753804	35.41746464008504	15.99568919092028	13.246624293614278
7	30.864446163788323	30.28531477801557	20.250988799763427	18.599250258432683
8	28.55959246651953	32.88635345358877	18.846065355511524	19.707988724380183
9	27.299878012871748	13.991936357645354	18.277546847773575	40.43063878170932
10	15.869776643055896	26.567150481371627	31.202670646974173	26.360402228598307
11	38.50570720014175	20.912463678701425	21.411130649599137	19.17069847155769
12	24.64689364555145	23.777336186821017	28.150425027755222	23.425345139872306
13	29.376914737881265	18.87476666248162	25.243789509642276	26.504529089994833
14	23.513984955505745	19.50571003754432	24.6535069298614	32.32679807708854
15	25.425153593188366	27.05783006602595	21.76536569199786	25.751650648787827
16	26.22997312436149	25.773335823116618	23.068309498756914	24.928381553764982
17	24.34318199199531	25.92831468121447	24.66254110689066	25.065962219899557
18	25.497680169271163	24.624365870446375	25.569250771152525	24.308703189129936
19	25.65471486476152	24.903765263721418	25.21101537206516	24.230504499451907
20	26.069862594641446	24.023886608713386	24.584214953985775	25.32203584265939
21	27.229748935362043	24.11180323731516	24.209248998456417	24.44919882886638
22	26.02955366144959	24.070250172394232	24.843825482225867	25.056370683930307
23	24.37359750528813	23.835638613479627	25.490940864035526	26.29982301719671
24	24.89873046626048	24.832385856680347	24.963609248731743	25.30527442832743
25	25.084651569487576	24.359895199668085	25.05769307982581	25.49776015101853
26	24.92423899625194	25.37870942125922	25.21511026532125	24.48194131716759
27	25.66902346473534	24.440212659918757	24.58800645984457	25.302757415501333
28	24.683126418028397	24.460448160705635	25.594679684910904	25.261745736355063
29	24.62258138527035	24.840416039972467	25.495768220868282	25.041234353888903
30	24.65336375455681	24.91644785479389	25.699709383842762	24.730479006806537
31	25.209294618502565	24.807211869375685	24.515703469549035	25.46779004257272
32	25.091837254951948	24.570066535804422	24.412075866112627	25.926020343131007
33	24.569238024829836	24.308766920743366	25.149896754786244	25.972098299640557
34	25.423815229306346	24.488490070614628	25.142631350855275	24.945063349223748
35	26.049149820276853	24.18334310551406	25.25326943176893	24.514237642440158
36	24.713271471180565	25.059716521783464	24.813521299105208	25.413490707930762
37	25.834947867540215	24.76349198256303	24.73130751778112	24.670252632115634
38	24.826092249019965	24.330196248882306	25.314021792401785	25.52968970969594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	28.0
2	44.0
3	44.0
4	235.5
5	427.0
6	427.0
7	567.5
8	708.0
9	776.0
10	844.0
11	844.0
12	1124.5
13	1405.0
14	2004.0
15	2603.0
16	2603.0
17	4052.0
18	5501.0
19	5501.0
20	6749.0
21	7997.0
22	9142.5
23	10288.0
24	10288.0
25	12145.5
26	14003.0
27	14003.0
28	18186.0
29	22369.0
30	27474.0
31	32579.0
32	32579.0
33	41922.5
34	51266.0
35	51266.0
36	58113.5
37	64961.0
38	74626.5
39	84292.0
40	84292.0
41	90490.5
42	96689.0
43	110166.5
44	123644.0
45	123644.0
46	126445.0
47	129246.0
48	129246.0
49	137935.0
50	146624.0
51	149616.5
52	152609.0
53	152609.0
54	144554.0
55	136499.0
56	136499.0
57	132791.0
58	129083.0
59	115982.0
60	102881.0
61	102881.0
62	97178.0
63	91475.0
64	75827.0
65	60179.0
66	60179.0
67	50715.0
68	41251.0
69	41251.0
70	32860.5
71	24470.0
72	19057.0
73	13644.0
74	13644.0
75	10187.5
76	6731.0
77	6731.0
78	6585.5
79	6440.0
80	4951.5
81	3463.0
82	3463.0
83	3327.5
84	3192.0
85	3192.0
86	2036.5
87	881.0
88	746.0
89	611.0
90	611.0
91	358.0
92	105.0
93	72.5
94	40.0
95	40.0
96	24.0
97	8.0
98	8.0
99	12.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00994213169500599
2	0.0
3	0.0
4	1.9119484028857676E-4
5	6.373161342952558E-5
6	6.373161342952558E-5
7	3.823896805771535E-4
8	0.0019756800163152934
9	0.003951360032630587
10	0.0014020954954495628
11	0.006181966502663982
12	3.823896805771535E-4
13	6.373161342952558E-5
14	6.373161342952558E-5
15	0.0
16	6.373161342952558E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2746322685905115E-4
22	1.2746322685905115E-4
23	6.373161342952558E-5
24	0.0
25	6.373161342952558E-5
26	6.373161342952558E-5
27	1.2746322685905115E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.373161342952558E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1569080.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.14360098101165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.25879899341504	45.06017985901514
2	12.59364517900298	14.141051796527687
3	3.418766987199068	5.758256687290795
4	1.347393794186869	3.0259015818047565
5	0.6506067295093606	1.8263702308567262
6	0.3846705019811426	1.2958072303436836
7	0.2387872350474634	0.9384462670704681
8	0.18292532050709648	0.8216068963099276
9	0.12230856507142533	0.6180158946537183
>10	0.6509605241156631	6.619840383116412
>50	0.06239890761317143	2.5142986724720107
>100	0.07284248048413876	9.042795355607891
>500	0.011092042022013662	4.2951579305753205
>1k	0.004802739844583235	4.042271214355534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3144	0.20037219262242842	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2612	0.16646697427792082	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2378	0.15155377673541184	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2237	0.14256761924184871	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2109	0.13440997272286945	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2078	0.13243429270655416	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1989	0.1267621791113264	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1968	0.12542381522930635	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1790	0.1140795880388508	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1741	0.11095673898080403	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1718	0.10949091187192495	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1705	0.10866240089734112	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1687	0.10751523185560967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.373161342952558E-5
2	0.0	0.0	0.0	0.0	6.373161342952558E-5
3	0.0	0.0	0.0	0.0	6.373161342952558E-5
4	0.0	0.0	0.0	0.0	6.373161342952558E-5
5	0.0	0.0	0.0	0.0	6.373161342952558E-5
6	0.0	0.0	0.0	0.0	1.9119484028857676E-4
7	0.0	0.0	0.0	0.0	1.9119484028857676E-4
8	0.0	0.0	0.0	0.0	1.9119484028857676E-4
9	0.0	0.0	0.0	0.0	1.9119484028857676E-4
10	0.0	0.0	0.0	0.0	1.9119484028857676E-4
11	0.0	0.0	0.0	0.0	2.549264537181023E-4
12	0.0	0.0	0.0	0.0	4.461212940066791E-4
13	0.0	0.0	0.0	0.0	4.461212940066791E-4
14	0.0	0.0	0.0	0.0	6.373161342952558E-4
15	0.0	0.0	0.0	6.373161342952558E-5	7.64779361154307E-4
16	0.0	0.0	0.0	1.9119484028857676E-4	8.285109745838325E-4
17	0.0	0.0	0.0	2.549264537181023E-4	8.285109745838325E-4
18	0.0	0.0	0.0	2.549264537181023E-4	8.922425880133582E-4
19	0.0	0.0	0.0	3.186580671476279E-4	8.922425880133582E-4
20	0.0	0.0	0.0	3.186580671476279E-4	0.0010197058148724092
21	0.0	0.0	0.0	4.461212940066791E-4	0.0011471690417314605
22	0.0	0.0	0.0	5.735845208657302E-4	0.001210900655160986
23	0.0	0.0	0.0	8.285109745838325E-4	0.0014020954954495628
24	0.0	0.0	0.0	8.922425880133582E-4	0.0014658271088790883
25	0.0	0.0	0.0	0.0012746322685905115	0.0014658271088790883
26	0.0	0.0	0.0	0.0014020954954495628	0.0014658271088790883
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	380	0.0	17.683577	28
CTAGCGG	385	0.0	17.45392	29
CATCGTT	295	0.0	17.35531	28
GTATCAA	2590	0.0	16.866947	1
ATCGTTT	315	0.0	16.253386	29
ATTGGAC	90	8.577339E-6	16.001465	3
TAGCGGC	435	0.0	15.4477215	30
AGGCCCG	590	0.0	14.915195	10
GCGCAAG	515	0.0	14.914464	1
TACCGTC	430	0.0	14.885085	7
ACGGTAT	595	0.0	14.791271	9
GTTCAAA	660	0.0	14.789704	1
CGCAAGA	535	0.0	14.654147	2
ACCGTCG	455	0.0	14.418903	8
CAAGACG	555	0.0	14.415735	4
AGGGGCG	400	0.0	14.399484	32
AGCGGCG	490	0.0	14.366832	31
CGACGGT	595	0.0	14.253407	7
GACGGTA	595	0.0	14.253407	8
CGACAAT	90	1.5352166E-4	14.221711	17
>>END_MODULE