FastQCFastQC Report
Thu 2 Feb 2017
SRR4063047_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063047_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989003
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29880.3021224404779359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19420.19635936392508416No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15920.16097018917030584No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13710.13862445311086014No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13160.13306329707796638No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12780.12922104381887617No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12440.12578323827126917No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12400.12537879055978596No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12400.12537879055978596No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12320.1245698951368196No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12150.12285099236301608No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11980.12113208958921258No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11100.11223423993658259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10970.11091978487426225No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10970.11091978487426225No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT10490.1060664123364641No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10460.1057630765528517No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10350.10465084534627296No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10270.1038419499233066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTA308.419056E-426.66502413
CATCGTT3400.021.64572528
ATCGTTT3500.021.48439229
TCGTTTA3750.019.62545830
CGTTTAT3800.019.36722831
CGGCATC4050.018.56769825
GCATCGT4050.018.56675727
GTATAGA450.00877036317.8054921
ATTCGTT450.008852567517.77668232
GGCATCG4250.017.3165826
ATAAGGC652.0993956E-417.231453
TATATAG652.1034556E-417.2270955
CGCATCG4350.016.91849713
GTATTAT600.002416326216.0249441
GGGTATA600.002416326216.0249441
TTAAGGG600.00244093816.0006313
AATAGCG2450.015.6701275
ATCGCCA4700.015.65781816
ATAGCGT2350.015.6562356
CAGTCGG4950.015.51497921
Produced by FastQC (version 0.11.5)