FastQCFastQC Report
Thu 2 Feb 2017
SRR4063047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989003
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33670.3404438611409672No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23800.2406463883324924No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15770.15945351025224394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14710.14873564589793964No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14560.1472189669798777No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13790.1394333485338265No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13230.13377108057306195No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12900.1304343869533257No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12720.1286143722516514No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12570.12709769333358947No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12370.1250754547761736No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12230.12365988778598246No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12220.12355877585811166No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12000.12133431344495416No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11750.11880651524818428No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11380.11506537391696486No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10700.1081897628217508No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10690.10808865089388No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10540.10657197197581807No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG10480.1059653004085933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA308.4187463E-426.66519230
ACGTAAC552.2190798E-623.271441
GTACCGT400.00447792320.0039526
ATGCGAT400.0044844819.99889426
CTCTTAC601.1384608E-418.6656341
CCGATAA1251.4733814E-1017.9235389
GTCTAAC1006.8155714E-817.5990261
ATACTGT1302.582965E-1017.2341736
GTTAAGA854.985137E-616.9402391
GTATAAT854.985137E-616.9402391
CGATAAC1354.4201443E-1016.59251410
TCCTATT3900.016.409352
CGTTTAT2700.015.99911631
TAACGAA1304.800313E-915.99911513
GTATAGG703.697302E-415.9991151
ATCGTTT2950.015.72794329
GTATTAG2650.015.6972451
CCTATTC3800.015.5812373
ATAACGA1451.2205419E-915.44742112
ACGAACG1357.910785E-915.40655615
Produced by FastQC (version 0.11.5)