FastQCFastQC Report
Thu 2 Feb 2017
SRR4063046_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063046_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752869
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT16300.21650512904635466No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11620.15434292021586757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT11600.15407726975078No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11340.15062381370464184No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10890.14464667824017194No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10640.14132604742657753No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10560.1402634455662273No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10300.13680998952008916No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10230.13588021289228272No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10070.1337550091715823No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9820.1304343783579879No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9250.1228633401029927No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9210.1223320391728176No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG9170.12180073824264248No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8810.11701902987106656No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8490.11276862242966572No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT7980.10599453556993314No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC7950.10559605987230183No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA7920.1051975841746705No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC7910.10506475894212673No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA7530.10001740010546324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA200.003753080531.99933630
CAATAGA401.5773368E-424.0346261
TGTACGT350.002066929622.855155
CCAACGT400.004482604619.99958629
TTAGGAC1650.018.4263083
GTAAGAC802.7905917E-618.0020183
ACTATAC450.00884180217.7797723
TAATACC550.0013648517.4530244
ATATACT550.0013648517.4530244
TAGATCC550.0013648517.4530244
GTGTAGG1201.6243575E-917.3583411
GTGTTAA652.0761872E-417.255631
AAGACGG3550.016.6746035
TAGGACG3000.016.5318934
CAAGACG3500.016.4557084
GTCCTAA2200.016.0230851
GCGCAAG3600.016.0230851
GCTTAAC600.002417477316.0230851
CTAAGGC805.61134E-516.0017953
ATACACT1001.3140907E-615.9986064
Produced by FastQC (version 0.11.5)