##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063046_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752869
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226715404671996	32.0	32.0	32.0	32.0	32.0
2	30.807286526606887	32.0	32.0	32.0	32.0	32.0
3	30.861395541588244	32.0	32.0	32.0	32.0	32.0
4	30.936529462628958	32.0	32.0	32.0	32.0	32.0
5	30.843848000116886	32.0	32.0	32.0	32.0	32.0
6	34.49969782259596	36.0	36.0	36.0	32.0	36.0
7	34.42956609981285	36.0	36.0	36.0	32.0	36.0
8	34.40033525088694	36.0	36.0	36.0	32.0	36.0
9	34.55061239073464	36.0	36.0	36.0	32.0	36.0
10	34.26000273619979	36.0	36.0	36.0	32.0	36.0
11	34.54381041057608	36.0	36.0	36.0	32.0	36.0
12	34.33781839868556	36.0	36.0	36.0	32.0	36.0
13	34.43898473705253	36.0	36.0	36.0	32.0	36.0
14	34.33709715767285	36.0	36.0	36.0	32.0	36.0
15	34.270534448888185	36.0	36.0	36.0	32.0	36.0
16	34.278093532872255	36.0	36.0	36.0	32.0	36.0
17	34.20788609970659	36.0	36.0	36.0	32.0	36.0
18	34.23341909415848	36.0	36.0	36.0	32.0	36.0
19	34.20930998619946	36.0	36.0	36.0	32.0	36.0
20	34.207425196149664	36.0	36.0	36.0	32.0	36.0
21	34.18449026324633	36.0	36.0	36.0	32.0	36.0
22	34.154190171198444	36.0	36.0	36.0	32.0	36.0
23	34.099150051336956	36.0	36.0	36.0	32.0	36.0
24	34.07068560400282	36.0	36.0	36.0	32.0	36.0
25	34.05477048463943	36.0	36.0	36.0	32.0	36.0
26	34.00248914485787	36.0	36.0	36.0	32.0	36.0
27	34.00782606270148	36.0	36.0	36.0	32.0	36.0
28	33.98229439650191	36.0	36.0	36.0	32.0	36.0
29	33.955903351047795	36.0	36.0	36.0	32.0	36.0
30	33.92418867027332	36.0	36.0	36.0	32.0	36.0
31	33.94018215652391	36.0	36.0	36.0	32.0	36.0
32	33.8910726832955	36.0	36.0	36.0	32.0	36.0
33	33.84683789610145	36.0	36.0	36.0	32.0	36.0
34	33.848794411776815	36.0	36.0	36.0	32.0	36.0
35	33.81553231704321	36.0	36.0	36.0	32.0	36.0
36	33.76302118960935	36.0	36.0	36.0	32.0	36.0
37	33.749588573842196	36.0	36.0	36.0	32.0	36.0
38	33.15193878350683	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	4.0
11	2.0
12	1.0
13	5.0
14	116.0
15	214.0
16	264.0
17	311.0
18	401.0
19	492.0
20	664.0
21	975.0
22	1551.0
23	2290.0
24	3381.0
25	5046.0
26	7446.0
27	10734.0
28	15324.0
29	20737.0
30	27550.0
31	37451.0
32	51049.0
33	76519.0
34	166899.0
35	323436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51069098414505	17.203454521758328	11.20033194049541	26.08552255360121
2	16.894898279225785	19.744913961289225	37.2712146422629	26.088973117222096
3	18.885489632415357	23.7452160594278	28.593650991858166	28.775643316298677
4	12.643986568337832	15.211171444966315	35.86753235712919	36.27730962956666
5	14.716922488404341	36.36294808651716	33.48183214056116	15.438297284517338
6	34.37003565033977	35.36596056679259	16.746494737457297	13.51750904541035
7	30.32081278416298	30.261174254750827	21.063425376791976	18.35458758429421
8	28.23500033870524	32.68213376333403	19.70308168640676	19.379784211553964
9	27.69552206293525	14.038033177086584	18.603236419616163	39.663208340362
10	16.377638576749177	26.008941806531823	31.179569326893958	26.433850289825045
11	37.91236086390564	20.799750285577666	22.17092155247988	19.11696729803682
12	25.2481553552809	23.590067144403637	28.362832152273675	22.798945348041787
13	29.890314550292313	18.89055233272537	25.177174480498522	26.0419586364838
14	23.892335914285297	20.06779814113012	24.49898581738337	31.540880127201216
15	25.332561022107853	27.281096929930026	22.05231965713633	25.334022390825794
16	25.99045444341276	25.246310162843	23.85432731455837	24.908908079185878
17	24.14459175811037	25.73039827828998	25.242314743470523	24.88269522012913
18	25.516743951894092	23.796057324341593	26.5301080310664	24.157090692697906
19	26.06681216268811	24.574835918477948	25.064722705216468	24.29362921361747
20	26.085160419638754	23.755841230118566	24.932322757352843	25.226675592889837
21	27.35991286271228	23.884649358757233	23.810131038009658	24.94530674052083
22	26.275842281718724	24.280188513833025	24.466550483770703	24.977418720677544
23	24.0929510936126	24.041810458193527	25.504432631179053	26.36080581701482
24	25.12460214985202	24.93477648659132	25.019660040701595	24.920961322855064
25	25.129288986956194	24.13735385661775	25.141776184257896	25.591580972168167
26	24.47625651939412	25.428891776064955	25.696841199116843	24.398010505424082
27	25.679771952615877	24.629064352187623	24.515093793253687	25.17606990194281
28	24.496038977944732	24.422317742509655	25.517775451758418	25.563867827787195
29	24.53058768363038	24.801309240266043	25.348597176153653	25.319505899949917
30	24.478383533549756	24.789201596064032	25.96087942915739	24.77153544122882
31	25.343832272016027	24.670911935901216	24.360619416129367	25.62463637595339
32	24.632156135557288	24.888786756159735	24.355467945746266	26.12358916253671
33	24.239695120898272	24.36309730175619	25.480889930302343	25.916317647043197
34	25.258238663535217	24.54276878606574	25.471710645171576	24.727281905227475
35	26.130199996014692	24.301740915159446	25.363692520242044	24.204366568583822
36	24.561363889966366	25.087806925721452	24.95722567309787	25.393603511214312
37	25.77874895391932	25.06674991033594	24.45544028373693	24.69906085200781
38	24.72962166958453	24.935510351551603	25.06116886325538	25.27369911560849
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	302.0
1	239.5
2	177.0
3	177.0
4	341.0
5	505.0
6	505.0
7	591.5
8	678.0
9	645.0
10	612.0
11	612.0
12	775.5
13	939.0
14	1217.0
15	1495.0
16	1495.0
17	2170.5
18	2846.0
19	2846.0
20	3445.5
21	4045.0
22	4322.5
23	4600.0
24	4600.0
25	5519.0
26	6438.0
27	6438.0
28	8457.5
29	10477.0
30	13532.0
31	16587.0
32	16587.0
33	21837.5
34	27088.0
35	27088.0
36	29930.0
37	32772.0
38	37493.5
39	42215.0
40	42215.0
41	44172.0
42	46129.0
43	51878.0
44	57627.0
45	57627.0
46	59784.0
47	61941.0
48	61941.0
49	65492.0
50	69043.0
51	69785.5
52	70528.0
53	70528.0
54	66683.0
55	62838.0
56	62838.0
57	61775.5
58	60713.0
59	54219.0
60	47725.0
61	47725.0
62	46035.5
63	44346.0
64	36611.5
65	28877.0
66	28877.0
67	24159.5
68	19442.0
69	19442.0
70	15687.5
71	11933.0
72	9313.5
73	6694.0
74	6694.0
75	5111.5
76	3529.0
77	3529.0
78	3488.5
79	3448.0
80	2711.0
81	1974.0
82	1974.0
83	2104.0
84	2234.0
85	2234.0
86	1448.0
87	662.0
88	568.0
89	474.0
90	474.0
91	352.0
92	230.0
93	200.0
94	170.0
95	170.0
96	159.0
97	148.0
98	148.0
99	268.0
100	388.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12312899056808023
2	0.055388121970754545
3	0.011954270928939829
4	0.0027893298834192936
5	1.3282523254377586E-4
6	1.3282523254377586E-4
7	0.0
8	2.6565046508755173E-4
9	0.0
10	1.3282523254377586E-4
11	0.0011954270928939829
12	0.0018595532556128622
13	0.01062601860350207
14	0.00610996069701369
15	0.019923784881566382
16	0.0077038634875390005
17	0.017134454998147086
18	0.003984756976313277
19	0.005578659766838587
20	0.004117582208857052
21	0.0045160579064883796
22	0.004383232673944603
23	0.005711484999382363
24	0.009696241975695638
25	0.013016872789290037
26	0.015274901742534227
27	0.005711484999382363
28	0.004383232673944603
29	0.008766465347889206
30	0.0022580289532441898
31	0.004117582208857052
32	0.0047817083715759314
33	0.00610996069701369
34	0.010227542905870742
35	0.013813824184552691
36	0.010758843836045846
37	0.007836688720082776
38	0.004648883139032156
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	752869.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.52821259314509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.32731919181167	48.51199945876545
2	9.831772393316369	11.312085848202377
3	2.563330963042542	4.423915457655081
4	1.007230675811348	2.3177672179364968
5	0.5694766418487838	1.6380486659553586
6	0.3388610524659008	1.1696442399477138
7	0.22973426943436867	0.9251341324365864
8	0.15000294942349016	0.6903521251226665
9	0.11280891802341203	0.5840725876608172
>10	0.6639267100011886	7.374293827252696
>50	0.08951537787567657	3.635389562917451
>100	0.105093040760326	12.47497704165888
>500	0.008602748911580294	3.4235095037546253
>1k	0.002325067273400079	1.5188103307338843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1630	0.21650512904635466	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1162	0.15434292021586757	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1160	0.15407726975078	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1134	0.15062381370464184	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1089	0.14464667824017194	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1064	0.14132604742657753	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1056	0.1402634455662273	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1030	0.13680998952008916	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1023	0.13588021289228272	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1007	0.1337550091715823	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	982	0.1304343783579879	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	925	0.1228633401029927	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	921	0.1223320391728176	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	917	0.12180073824264248	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	881	0.11701902987106656	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	849	0.11276862242966572	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	798	0.10599453556993314	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	795	0.10559605987230183	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	792	0.1051975841746705	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	791	0.10506475894212673	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	753	0.10001740010546324	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	1.3282523254377586E-4	0.0	0.0
7	0.0	0.0	1.3282523254377586E-4	0.0	0.0
8	0.0	0.0	1.3282523254377586E-4	0.0	0.0
9	0.0	0.0	1.3282523254377586E-4	0.0	0.0
10	0.0	0.0	1.3282523254377586E-4	0.0	0.0
11	0.0	0.0	1.3282523254377586E-4	0.0	0.0
12	0.0	0.0	1.3282523254377586E-4	0.0	1.3282523254377586E-4
13	0.0	0.0	1.3282523254377586E-4	0.0	1.3282523254377586E-4
14	0.0	0.0	1.3282523254377586E-4	0.0	1.3282523254377586E-4
15	0.0	0.0	1.3282523254377586E-4	0.0	5.313009301751035E-4
16	0.0	0.0	1.3282523254377586E-4	1.3282523254377586E-4	7.969513952626552E-4
17	0.0	0.0	1.3282523254377586E-4	1.3282523254377586E-4	7.969513952626552E-4
18	0.0	0.0	1.3282523254377586E-4	1.3282523254377586E-4	7.969513952626552E-4
19	0.0	0.0	1.3282523254377586E-4	2.6565046508755173E-4	7.969513952626552E-4
20	0.0	0.0	1.3282523254377586E-4	2.6565046508755173E-4	7.969513952626552E-4
21	0.0	0.0	1.3282523254377586E-4	3.984756976313276E-4	9.297766278064311E-4
22	0.0	0.0	1.3282523254377586E-4	5.313009301751035E-4	9.297766278064311E-4
23	0.0	0.0	1.3282523254377586E-4	6.641261627188794E-4	9.297766278064311E-4
24	0.0	0.0	1.3282523254377586E-4	0.0013282523254377588	9.297766278064311E-4
25	0.0	0.0	1.3282523254377586E-4	0.0019923784881566383	9.297766278064311E-4
26	0.0	0.0	1.3282523254377586E-4	0.003054980348506845	0.001062601860350207
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTA	20	0.0037530805	31.999336	30
CAATAGA	40	1.5773368E-4	24.034626	1
TGTACGT	35	0.0020669296	22.85515	5
CCAACGT	40	0.0044826046	19.999586	29
TTAGGAC	165	0.0	18.426308	3
GTAAGAC	80	2.7905917E-6	18.002018	3
ACTATAC	45	0.008841802	17.779772	3
TAATACC	55	0.00136485	17.453024	4
ATATACT	55	0.00136485	17.453024	4
TAGATCC	55	0.00136485	17.453024	4
GTGTAGG	120	1.6243575E-9	17.358341	1
GTGTTAA	65	2.0761872E-4	17.25563	1
AAGACGG	355	0.0	16.674603	5
TAGGACG	300	0.0	16.531893	4
CAAGACG	350	0.0	16.455708	4
GTCCTAA	220	0.0	16.023085	1
GCGCAAG	360	0.0	16.023085	1
GCTTAAC	60	0.0024174773	16.023085	1
CTAAGGC	80	5.61134E-5	16.001795	3
ATACACT	100	1.3140907E-6	15.998606	4
>>END_MODULE