FastQCFastQC Report
Thu 2 Feb 2017
SRR4063046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752869
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20590.27348715380763455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15270.20282413009434577No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12480.16576589021463228No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11860.15753072579691818No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11790.15660094916911177No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10950.14544362963543458No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10680.14185734835675262No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10220.13574738765973893No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10000.13282523254377587No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9800.13016872789290035No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9540.12671527184676218No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9490.1260511456840433No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9440.12538701952132442No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9140.12140226254501116No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9040.1200740102195734No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8970.11914423359176696No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8940.11874575789413563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT8780.11662055417343523No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8720.11582360277817257No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG7970.10586171033738936No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA7950.10559605987230183No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG7870.10453345801195162No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG7590.10081435150072589No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA401.5930411E-423.9995542
TAGTGTC350.002066106522.8567164
CGTGATT1550.019.61253528
GTATAAT852.3904067E-718.825681
CGTCTTA601.1376827E-418.66631915
TCTAGCG2350.018.38263528
CTAGCGG2350.018.38263529
CCGTGAT1700.017.88201927
TCTAGAT2150.017.8601322
GTATAGG450.00884170417.7798061
TAGCGGC2450.017.63232430
CAGATAG550.00136422417.4542185
GATACTG753.2318152E-517.0663495
ATACAGC854.980211E-616.940866
TCCGTGA1800.016.88857326
AGGGGCG1551.0913936E-1116.5158232
ATTTAGA1750.016.459021
GTCTTAC703.6910712E-416.0018271
GTCCTAG908.563646E-616.0018251
CTATTAT600.002438996516.0018251
Produced by FastQC (version 0.11.5)