##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063046_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752869
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23833628426725	32.0	32.0	32.0	32.0	32.0
2	31.301258253427886	32.0	32.0	32.0	32.0	32.0
3	31.370377847939018	32.0	32.0	32.0	32.0	32.0
4	31.47143792611995	32.0	32.0	32.0	32.0	32.0
5	31.3972576902489	32.0	32.0	32.0	32.0	32.0
6	34.9253681583383	36.0	36.0	36.0	36.0	36.0
7	34.9432570606573	36.0	36.0	36.0	36.0	36.0
8	34.88365705056258	36.0	36.0	36.0	32.0	36.0
9	34.993005423254246	36.0	36.0	36.0	36.0	36.0
10	34.83298820910411	36.0	36.0	36.0	32.0	36.0
11	35.02981926470608	36.0	36.0	36.0	36.0	36.0
12	34.907278689918165	36.0	36.0	36.0	32.0	36.0
13	34.96951926563585	36.0	36.0	36.0	36.0	36.0
14	34.907023665471684	36.0	36.0	36.0	32.0	36.0
15	34.87716986620514	36.0	36.0	36.0	32.0	36.0
16	34.88130471569423	36.0	36.0	36.0	32.0	36.0
17	34.846608108449146	36.0	36.0	36.0	32.0	36.0
18	34.84878777051519	36.0	36.0	36.0	32.0	36.0
19	34.85013727487783	36.0	36.0	36.0	32.0	36.0
20	34.836979607342045	36.0	36.0	36.0	32.0	36.0
21	34.815686394312955	36.0	36.0	36.0	32.0	36.0
22	34.792317122899206	36.0	36.0	36.0	32.0	36.0
23	34.739014357079384	36.0	36.0	36.0	32.0	36.0
24	34.70591298087715	36.0	36.0	36.0	32.0	36.0
25	34.696206112882855	36.0	36.0	36.0	32.0	36.0
26	34.62553113489864	36.0	36.0	36.0	32.0	36.0
27	34.611273674437385	36.0	36.0	36.0	32.0	36.0
28	34.57443990920067	36.0	36.0	36.0	32.0	36.0
29	34.538917129009164	36.0	36.0	36.0	32.0	36.0
30	34.50000996189244	36.0	36.0	36.0	32.0	36.0
31	34.51958043165544	36.0	36.0	36.0	32.0	36.0
32	34.47237700051404	36.0	36.0	36.0	32.0	36.0
33	34.432204008931166	36.0	36.0	36.0	32.0	36.0
34	34.42499956831799	36.0	36.0	36.0	32.0	36.0
35	34.38552258095366	36.0	36.0	36.0	32.0	36.0
36	34.35861883010192	36.0	36.0	36.0	32.0	36.0
37	34.341916057109536	36.0	36.0	36.0	32.0	36.0
38	33.909672200608604	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	12.0
22	59.0
23	175.0
24	489.0
25	1185.0
26	2711.0
27	5068.0
28	8836.0
29	13979.0
30	21342.0
31	30666.0
32	44620.0
33	70781.0
34	172160.0
35	380779.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.93137939920776	18.31587420929164	11.905989059598356	26.846757331902243
2	15.881514579561651	20.67929480427538	36.635988465456805	26.803202150706166
3	17.885847338647228	24.569745865482574	28.908880562222645	28.63552623364755
4	12.114856635085253	16.108114426281332	35.90983291913999	35.86719601949343
5	14.20034561125508	36.90070915391655	33.12010456002306	15.778840674805311
6	34.285558690235206	35.984794147181184	16.328493175430488	13.401153987153128
7	29.98606652206311	30.68074802454099	20.909249093128498	18.423936360267408
8	28.136419001218027	32.93068936066212	19.42480444493443	19.508087193185425
9	27.64001184835697	13.78115340832865	18.506549139466586	40.072285603847796
10	16.120505644468235	26.188632430156428	31.232989887907596	26.457872037467737
11	37.902278849576795	20.636632679627436	22.217986387473502	19.243102083322263
12	25.011954286807246	23.764325220357353	28.290085380172886	22.93363511266251
13	29.818852226026944	18.93922690093589	25.19106985838117	26.050851014655997
14	23.74782332650169	19.913026037730337	24.496691987583496	31.84245864818448
15	25.29457315947396	27.4010485223857	21.971684316926318	25.332694001214023
16	25.940403895503593	25.271495135933524	23.815197351992648	24.972903616570235
17	24.23409650284445	25.885778269526305	25.031180723339652	24.84894450428959
18	25.550925858283446	23.877859229162045	26.39383478400625	24.177380128548258
19	26.011298114280173	24.587278796178353	25.1165873478653	24.284835741676176
20	26.142529444033425	23.687520670926816	24.976722377996705	25.19322750704306
21	27.38823723180854	23.924013139106908	23.79583644920019	24.891913179884362
22	26.061373226949176	24.303165623767214	24.556994643158372	25.078466506125235
23	24.017224799035155	23.82183862244112	25.632116121285538	26.52882045723819
24	25.072489370660765	24.800064818713484	25.06637940996375	25.061066400662003
25	25.01487644580458	24.147128049007264	25.24864916559078	25.58934633959738
26	24.613445366989474	25.1827343136721	25.641778317343388	24.562042001995035
27	25.700122730677887	24.430046170112156	24.578013675704106	25.291817423505847
28	24.51754554909287	24.210187960986573	25.69172060477985	25.580545885140708
29	24.637619559312444	24.503997375373405	25.60910331013762	25.24927975517653
30	24.433334351660115	24.65183185919463	26.035339481370595	24.87949430777466
31	25.406146354810733	24.385782918409443	24.460165048633957	25.747905678145866
32	24.832474175454163	24.675209100122334	24.404511276198118	26.08780544822539
33	24.207531456335694	24.210586436684203	25.651341734086543	25.930540372893557
34	25.253264512152846	24.4038471500354	25.690923653384584	24.65196468442717
35	26.262736279485544	24.13726690832004	25.42009300422783	24.17990380796659
36	24.719705553024497	24.97632390229907	24.961580301486713	25.342390243189715
37	25.847923078251327	24.714658194187834	24.608132357687726	24.829286369873113
38	24.733850733263644	24.55480184415043	25.340863658521357	25.370483764064566
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	124.0
1	108.5
2	93.0
3	93.0
4	319.0
5	545.0
6	545.0
7	643.0
8	741.0
9	702.0
10	663.0
11	663.0
12	842.5
13	1022.0
14	1281.0
15	1540.0
16	1540.0
17	2324.0
18	3108.0
19	3108.0
20	3719.5
21	4331.0
22	4585.0
23	4839.0
24	4839.0
25	5603.5
26	6368.0
27	6368.0
28	8354.0
29	10340.0
30	13403.0
31	16466.0
32	16466.0
33	21896.0
34	27326.0
35	27326.0
36	30196.0
37	33066.0
38	37285.0
39	41504.0
40	41504.0
41	43404.5
42	45305.0
43	51103.0
44	56901.0
45	56901.0
46	59545.0
47	62189.0
48	62189.0
49	66080.5
50	69972.0
51	70418.0
52	70864.0
53	70864.0
54	67157.0
55	63450.0
56	63450.0
57	62110.0
58	60770.0
59	54280.0
60	47790.0
61	47790.0
62	46262.5
63	44735.0
64	36859.5
65	28984.0
66	28984.0
67	24343.0
68	19702.0
69	19702.0
70	15967.0
71	12232.0
72	9464.0
73	6696.0
74	6696.0
75	4963.0
76	3230.0
77	3230.0
78	3302.0
79	3374.0
80	2597.5
81	1821.0
82	1821.0
83	1837.5
84	1854.0
85	1854.0
86	1170.0
87	486.0
88	410.5
89	335.0
90	335.0
91	195.0
92	55.0
93	37.5
94	20.0
95	20.0
96	15.5
97	11.0
98	11.0
99	14.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008367989650257882
2	0.0
3	0.0
4	0.0
5	0.0
6	1.3282523254377586E-4
7	7.969513952626552E-4
8	0.0015939027905253105
9	0.002922155115963069
10	0.001062601860350207
11	0.004383232673944603
12	1.3282523254377586E-4
13	3.984756976313276E-4
14	0.0
15	0.0
16	1.3282523254377586E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	2.6565046508755173E-4
22	0.0
23	1.3282523254377586E-4
24	0.0
25	1.3282523254377586E-4
26	0.0
27	1.3282523254377586E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.6565046508755173E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	752869.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.64050932392336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.18629125192226	46.28527614025184
2	10.601957336912138	11.797966121125949
3	2.6668184383342086	4.451494085500357
4	1.1270019795407207	2.508278566028622
5	0.583834550861573	1.6242425885420981
6	0.3566642146065469	1.1906987134995222
7	0.24368268460572595	0.9491040079418499
8	0.17124310072937887	0.7622442674232441
9	0.1340067163333986	0.6710581754655136
>10	0.70688593214842	7.5078761871061666
>50	0.09820169609392845	3.869412852272272
>100	0.10996642187483602	12.590797751055682
>500	0.011524865174510736	4.404314410582359
>1k	0.001920810862418456	1.387236133204538
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2059	0.27348715380763455	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1527	0.20282413009434577	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1248	0.16576589021463228	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1186	0.15753072579691818	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1179	0.15660094916911177	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1095	0.14544362963543458	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1068	0.14185734835675262	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1022	0.13574738765973893	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1000	0.13282523254377587	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	980	0.13016872789290035	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	954	0.12671527184676218	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	949	0.1260511456840433	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	944	0.12538701952132442	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	914	0.12140226254501116	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	904	0.1200740102195734	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	897	0.11914423359176696	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	894	0.11874575789413563	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	878	0.11662055417343523	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	872	0.11582360277817257	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	797	0.10586171033738936	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	795	0.10559605987230183	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	787	0.10453345801195162	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	759	0.10081435150072589	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.6565046508755173E-4
13	0.0	0.0	0.0	0.0	2.6565046508755173E-4
14	0.0	0.0	0.0	0.0	2.6565046508755173E-4
15	0.0	0.0	0.0	0.0	2.6565046508755173E-4
16	0.0	0.0	0.0	1.3282523254377586E-4	5.313009301751035E-4
17	0.0	0.0	0.0	1.3282523254377586E-4	5.313009301751035E-4
18	0.0	0.0	0.0	1.3282523254377586E-4	6.641261627188794E-4
19	0.0	0.0	0.0	2.6565046508755173E-4	6.641261627188794E-4
20	0.0	0.0	0.0	2.6565046508755173E-4	9.297766278064311E-4
21	0.0	0.0	0.0	3.984756976313276E-4	9.297766278064311E-4
22	0.0	0.0	0.0	5.313009301751035E-4	9.297766278064311E-4
23	0.0	0.0	0.0	6.641261627188794E-4	9.297766278064311E-4
24	0.0	0.0	0.0	0.0015939027905253105	9.297766278064311E-4
25	0.0	0.0	0.0	0.0022580289532441898	9.297766278064311E-4
26	0.0	0.0	0.0	0.003586281278681949	9.297766278064311E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTATA	40	1.5930411E-4	23.999554	2
TAGTGTC	35	0.0020661065	22.856716	4
CGTGATT	155	0.0	19.612535	28
GTATAAT	85	2.3904067E-7	18.82568	1
CGTCTTA	60	1.1376827E-4	18.666319	15
TCTAGCG	235	0.0	18.382635	28
CTAGCGG	235	0.0	18.382635	29
CCGTGAT	170	0.0	17.882019	27
TCTAGAT	215	0.0	17.860132	2
GTATAGG	45	0.008841704	17.779806	1
TAGCGGC	245	0.0	17.632324	30
CAGATAG	55	0.001364224	17.454218	5
GATACTG	75	3.2318152E-5	17.066349	5
ATACAGC	85	4.980211E-6	16.94086	6
TCCGTGA	180	0.0	16.888573	26
AGGGGCG	155	1.0913936E-11	16.51582	32
ATTTAGA	175	0.0	16.45902	1
GTCTTAC	70	3.6910712E-4	16.001827	1
GTCCTAG	90	8.563646E-6	16.001825	1
CTATTAT	60	0.0024389965	16.001825	1
>>END_MODULE