FastQCFastQC Report
Thu 2 Feb 2017
SRR4063045_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063045_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883177
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30550.16222585556216967No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27560.14634843140076584No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26870.14268441044044186No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24870.1320640598308072No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24260.1288248528948686No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC23290.12367398284919578No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22910.12165611623336521No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22110.11740797598951133No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21550.11443427781881363No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21240.11278812347432025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19660.10439804649270887No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC19500.10354841844393808No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18940.10057472027324038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA400.00448577419.9990379
GTATCAA28950.017.9253921
CAAGACG6500.017.71944
CGCAAGA6400.017.5052012
AAGACGG6800.017.1724515
GAACCGT753.241228E-517.0631266
CTTATAC1601.8189894E-1216.998283
GCGCAAG6800.016.4877471
TCGCGTA3150.016.2531859
TACCGTC4650.016.1699777
ACGGATA703.6985707E-415.99965524
CGGACCA7300.015.7800629
CGCGTAA3300.015.51440510
ACCGTCG4850.015.5031758
ACGGACC7450.015.4611118
CCGTCGT4900.015.34629
GCGTAAC3350.015.28365911
ATACCGT4950.015.1887686
CGTCGTA4850.015.17452810
TCTAGCG5300.015.09361328
Produced by FastQC (version 0.11.5)