##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063045_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1883177
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264150422397893	32.0	32.0	32.0	32.0	32.0
2	30.859595247817918	32.0	32.0	32.0	32.0	32.0
3	30.911931804604666	32.0	32.0	32.0	32.0	32.0
4	30.986621013319514	32.0	32.0	32.0	32.0	32.0
5	30.87499794230707	32.0	32.0	32.0	32.0	32.0
6	34.54341466574836	36.0	36.0	36.0	32.0	36.0
7	34.47864486450291	36.0	36.0	36.0	32.0	36.0
8	34.46272973809685	36.0	36.0	36.0	32.0	36.0
9	34.61632284166597	36.0	36.0	36.0	32.0	36.0
10	34.3171167659758	36.0	36.0	36.0	32.0	36.0
11	34.59295647727218	36.0	36.0	36.0	32.0	36.0
12	34.4041600975373	36.0	36.0	36.0	32.0	36.0
13	34.49467097357285	36.0	36.0	36.0	32.0	36.0
14	34.38771448461828	36.0	36.0	36.0	32.0	36.0
15	34.334114106108984	36.0	36.0	36.0	32.0	36.0
16	34.34654522649756	36.0	36.0	36.0	32.0	36.0
17	34.270610250656205	36.0	36.0	36.0	32.0	36.0
18	34.29482358801111	36.0	36.0	36.0	32.0	36.0
19	34.28820817161637	36.0	36.0	36.0	32.0	36.0
20	34.27947983646784	36.0	36.0	36.0	32.0	36.0
21	34.261938734383435	36.0	36.0	36.0	32.0	36.0
22	34.24229905101857	36.0	36.0	36.0	32.0	36.0
23	34.17485079734937	36.0	36.0	36.0	32.0	36.0
24	34.166863762673394	36.0	36.0	36.0	32.0	36.0
25	34.141681849342895	36.0	36.0	36.0	32.0	36.0
26	34.08987524805156	36.0	36.0	36.0	32.0	36.0
27	34.09525445563534	36.0	36.0	36.0	32.0	36.0
28	34.072913486092915	36.0	36.0	36.0	32.0	36.0
29	34.04619215294154	36.0	36.0	36.0	32.0	36.0
30	34.021599138052345	36.0	36.0	36.0	32.0	36.0
31	34.041277054679405	36.0	36.0	36.0	32.0	36.0
32	33.99868891771724	36.0	36.0	36.0	32.0	36.0
33	33.96130422153627	36.0	36.0	36.0	32.0	36.0
34	33.97712004766414	36.0	36.0	36.0	32.0	36.0
35	33.94087597713863	36.0	36.0	36.0	32.0	36.0
36	33.89125291993265	36.0	36.0	36.0	32.0	36.0
37	33.89815827189903	36.0	36.0	36.0	32.0	36.0
38	33.311506034748724	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	2.0
8	4.0
9	8.0
10	13.0
11	21.0
12	8.0
13	11.0
14	146.0
15	374.0
16	525.0
17	625.0
18	728.0
19	1044.0
20	1395.0
21	2096.0
22	3306.0
23	5203.0
24	7834.0
25	12361.0
26	18147.0
27	26334.0
28	36297.0
29	49286.0
30	66676.0
31	89727.0
32	122927.0
33	184907.0
34	407133.0
35	846038.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.992280373434276	16.8531888650235	11.304999681007166	25.84953108053506
2	17.074955131481495	18.852375401532065	37.64120699236943	26.431462474617014
3	19.260409535832128	22.93547237568914	27.893726954514726	29.91039113396401
4	12.571370916351587	14.788648625685033	35.72645397000722	36.913526487956155
5	14.98718123000718	36.23230117631066	33.29773743930466	15.4827801543775
6	35.21867867759432	35.00685544723961	16.490297763244392	13.284168111921682
7	31.302739997355534	29.962770360937924	20.11398822309321	18.620501418613333
8	28.680985422991327	32.72692708203344	18.861218657254174	19.730868837721058
9	27.533872316699192	14.203671990123063	18.111965165213004	40.15049052796474
10	16.003608810467888	26.272150826611952	31.05005445607909	26.674185906841075
11	38.359781897584476	20.93920193377911	21.567935036576973	19.13308113205944
12	25.104958457373538	23.416011461792785	28.170233962039713	23.30879611879396
13	29.570726531019787	18.94088786374483	24.963792150212523	26.524593455022867
14	23.69355040002974	19.516884096262054	24.680547110040706	32.1090183936675
15	25.55994051096829	26.781112232432147	21.871354968927605	25.787592287671963
16	26.391222699724047	25.564593392913665	23.06314353874881	24.98104036861347
17	24.54416872170723	25.763330498855673	24.49057744205476	25.201923337382333
18	25.508280923155517	24.5886090906146	25.524477938337697	24.378632047892186
19	25.79254294030709	24.740669381807212	24.97529236264492	24.491495315240776
20	26.039992735159384	23.938605724142107	24.61607335364798	25.40532818705053
21	27.38643925043559	23.95097997044184	24.060800810592507	24.601779968530064
22	26.324985223213126	23.80578867681242	24.811566095120554	25.057660004853904
23	24.654100475361247	23.826692561666583	25.319372550769014	26.19983441220315
24	24.9653589366508	24.693328999549095	24.88171052764354	25.459601536156562
25	25.187139304724504	24.290718931476263	24.82374860714396	25.698393156655275
26	24.948189142823757	25.287256812955107	25.234835166586112	24.529718877635023
27	25.817319743555974	24.35055210240767	24.450287416143738	25.38184073789262
28	24.924710591816435	24.383094175618815	25.365746412634284	25.326448819930462
29	24.76559550028333	24.72576465920998	25.33618557560611	25.172454264900583
30	24.717370189956334	24.910666729682752	25.548810828004846	24.823152252356067
31	25.35785996158399	24.771742299405915	24.32189155044239	25.548506188567703
32	25.15609043447229	24.525196716166334	24.26566998204502	26.05304286731636
33	24.64243304846936	24.240150313010485	24.987201301749757	26.130215336770394
34	25.636376575606263	24.437922679914134	24.946811505072425	24.978889239407177
35	26.22548655119596	24.00055448034994	25.245426599335897	24.528532369118206
36	24.757350906571535	24.937608312447587	24.68485492571685	25.620185855264033
37	25.96332521323878	24.676908925890462	24.512860710903922	24.846905149966837
38	24.83760474971589	24.464116916083395	25.008815438700836	25.689462895499876
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	36.0
1	68.5
2	101.0
3	101.0
4	286.5
5	472.0
6	472.0
7	586.0
8	700.0
9	704.0
10	708.0
11	708.0
12	1020.5
13	1333.0
14	1932.0
15	2531.0
16	2531.0
17	3971.5
18	5412.0
19	5412.0
20	6841.5
21	8271.0
22	9593.5
23	10916.0
24	10916.0
25	13408.0
26	15900.0
27	15900.0
28	20775.0
29	25650.0
30	31250.0
31	36850.0
32	36850.0
33	47900.0
34	58950.0
35	58950.0
36	67243.0
37	75536.0
38	87381.0
39	99226.0
40	99226.0
41	107178.5
42	115131.0
43	131875.0
44	148619.0
45	148619.0
46	152990.5
47	157362.0
48	157362.0
49	167777.5
50	178193.0
51	181014.0
52	183835.0
53	183835.0
54	176208.5
55	168582.0
56	168582.0
57	163005.5
58	157429.0
59	141551.0
60	125673.0
61	125673.0
62	117425.0
63	109177.0
64	91394.5
65	73612.0
66	73612.0
67	61554.5
68	49497.0
69	49497.0
70	39163.5
71	28830.0
72	22557.0
73	16284.0
74	16284.0
75	12229.5
76	8175.0
77	8175.0
78	7710.0
79	7245.0
80	5727.5
81	4210.0
82	4210.0
83	4146.0
84	4082.0
85	4082.0
86	2778.5
87	1475.0
88	1253.0
89	1031.0
90	1031.0
91	775.0
92	519.0
93	450.0
94	381.0
95	381.0
96	351.0
97	321.0
98	321.0
99	621.5
100	922.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11985065662972733
2	0.053685872331703285
3	0.01146997865840545
4	0.0030267999237458825
5	5.3101753048173385E-5
6	1.5930525914452015E-4
7	0.0
8	3.186105182890403E-4
9	1.0620350609634677E-4
10	4.248140243853871E-4
11	0.0013275438262043344
12	0.0027081894054568423
13	0.015824322408355667
14	0.01019553658524929
15	0.025329536203978705
16	0.012160301448031704
17	0.02166551524365474
18	0.006053599847491765
19	0.008283873475515048
20	0.007168736661503406
21	0.005841192835299072
22	0.008283873475515048
23	0.009983129573056596
24	0.01571811890225932
25	0.01885112233210155
26	0.01948834336867963
27	0.010248638338297462
28	0.005841192835299072
29	0.011363775152309102
30	0.0031861051828904027
31	0.005734989329202725
32	0.007115634908455233
33	0.009186603277333994
34	0.01332854001509152
35	0.017682883765041735
36	0.01667395045712644
37	0.010301740091345636
38	0.006212905106636285
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1883177.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.043402235584594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04258704670528	42.17686923270717
2	13.174594717313257	14.239998431971362
3	4.147303680754834	6.724032030364619
4	1.6853784577293407	3.643343436410239
5	0.8466771266976203	2.2878656260894257
6	0.4988255416397313	1.6174937635331608
7	0.308720412797736	1.1679011013014635
8	0.21910652369291952	0.9473009593901676
9	0.1666193486568453	0.81042088317237
>10	0.7620355510819928	7.239772938388637
>50	0.06696069747944175	2.5576330612160123
>100	0.0646767622701114	7.763734655517553
>500	0.010877572753889246	4.014128946621833
>1k	0.005636560427015337	4.809504933316006
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3055	0.16222585556216967	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2756	0.14634843140076584	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2687	0.14268441044044186	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2487	0.1320640598308072	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2426	0.1288248528948686	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2329	0.12367398284919578	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2291	0.12165611623336521	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2211	0.11740797598951133	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2155	0.11443427781881363	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2124	0.11278812347432025	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1966	0.10439804649270887	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1950	0.10354841844393808	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1894	0.10057472027324038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.310175304817338E-5
2	0.0	0.0	0.0	0.0	5.310175304817338E-5
3	0.0	0.0	0.0	0.0	5.310175304817338E-5
4	0.0	0.0	0.0	0.0	5.310175304817338E-5
5	0.0	0.0	0.0	5.310175304817338E-5	5.310175304817338E-5
6	0.0	0.0	0.0	1.0620350609634676E-4	5.310175304817338E-5
7	0.0	5.310175304817338E-5	0.0	1.5930525914452013E-4	5.310175304817338E-5
8	0.0	5.310175304817338E-5	0.0	1.5930525914452013E-4	5.310175304817338E-5
9	0.0	5.310175304817338E-5	0.0	1.5930525914452013E-4	5.310175304817338E-5
10	0.0	5.310175304817338E-5	0.0	1.5930525914452013E-4	5.310175304817338E-5
11	0.0	5.310175304817338E-5	0.0	1.5930525914452013E-4	5.310175304817338E-5
12	0.0	1.0620350609634676E-4	0.0	1.5930525914452013E-4	1.0620350609634676E-4
13	0.0	1.0620350609634676E-4	0.0	1.5930525914452013E-4	1.0620350609634676E-4
14	0.0	1.0620350609634676E-4	0.0	1.5930525914452013E-4	2.124070121926935E-4
15	0.0	1.0620350609634676E-4	0.0	2.124070121926935E-4	2.124070121926935E-4
16	0.0	1.0620350609634676E-4	0.0	2.124070121926935E-4	2.124070121926935E-4
17	0.0	1.0620350609634676E-4	0.0	2.655087652408669E-4	2.124070121926935E-4
18	0.0	1.0620350609634676E-4	0.0	3.1861051828904025E-4	2.124070121926935E-4
19	0.0	1.0620350609634676E-4	0.0	3.7171227133721366E-4	2.124070121926935E-4
20	0.0	1.0620350609634676E-4	0.0	4.24814024385387E-4	2.124070121926935E-4
21	0.0	1.0620350609634676E-4	0.0	4.7791577743356043E-4	3.1861051828904025E-4
22	0.0	1.0620350609634676E-4	0.0	4.7791577743356043E-4	4.24814024385387E-4
23	0.0	1.0620350609634676E-4	0.0	6.372210365780805E-4	4.24814024385387E-4
24	0.0	1.0620350609634676E-4	0.0	0.0010089333079152942	4.24814024385387E-4
25	0.0	1.0620350609634676E-4	0.0	0.0011682385670598143	4.24814024385387E-4
26	0.0	1.0620350609634676E-4	0.0	0.0014337473323006813	4.24814024385387E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGA	40	0.004485774	19.999037	9
GTATCAA	2895	0.0	17.925392	1
CAAGACG	650	0.0	17.7194	4
CGCAAGA	640	0.0	17.505201	2
AAGACGG	680	0.0	17.172451	5
GAACCGT	75	3.241228E-5	17.063126	6
CTTATAC	160	1.8189894E-12	16.99828	3
GCGCAAG	680	0.0	16.487747	1
TCGCGTA	315	0.0	16.253185	9
TACCGTC	465	0.0	16.169977	7
ACGGATA	70	3.6985707E-4	15.999655	24
CGGACCA	730	0.0	15.780062	9
CGCGTAA	330	0.0	15.514405	10
ACCGTCG	485	0.0	15.503175	8
ACGGACC	745	0.0	15.461111	8
CCGTCGT	490	0.0	15.3462	9
GCGTAAC	335	0.0	15.283659	11
ATACCGT	495	0.0	15.188768	6
CGTCGTA	485	0.0	15.174528	10
TCTAGCG	530	0.0	15.093613	28
>>END_MODULE