FastQCFastQC Report
Thu 2 Feb 2017
SRR4063045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883177
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29790.1581901223305085No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28410.15086208040986057No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26990.14332163147701996No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26100.1385955754557325No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25010.1328074843734816No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT23870.12675388452598987No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23340.12393949161443668No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23070.12250574428213598No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22000.11682385670598143No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21310.11315983574565747No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20860.11077025685848968No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC20670.10976132355057437No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20160.10705313414511752No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19260.10227397637078194No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC19050.10115883955677028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5600.020.28522728
CTAGCGG5650.020.10571129
TAGCGGC6300.018.28527530
GTATTAG3550.017.1268021
CAAGACG5800.017.1039454
AAGACGG5900.017.086155
GCGCAAG5350.016.7477051
ATCGTTT4050.016.1971429
AGCGGCG7150.016.11150231
GTATCAA28500.015.9438991
CGCAAGA5700.015.7193372
CGCCGGT7150.015.6656237
GCGGCGC7700.015.58404232
CATCGTT4450.015.46030128
ACCCGTC859.4306684E-515.06006058
ATACCGT4700.014.9799556
CGGTCCA7700.014.96147410
GACGGAC6550.014.9019877
TACCGTC4300.014.8849447
TTTTCGG6050.014.80956129
Produced by FastQC (version 0.11.5)