FastQCFastQC Report
Thu 2 Feb 2017
SRR4063044_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063044_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686767
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA31710.4617286503282773No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC25470.3708681401406882No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT24170.3519388671849405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15460.22511273838143067No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG14210.2069115143855194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT9500.1383293023689257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.11590539440596301No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7740.11270197898268262No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7370.10731441667989289No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT7270.10585831876021999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1350.023.70240828
CGGTCCA1350.023.70240810
CTAGCGG1450.022.06936829
GCGTAAC902.0359948E-819.55448711
TAGCGGC1750.019.2003530
CGGGTAA507.1945484E-419.19895224
CGCGTAA852.393881E-718.822510
CGCCGGT1700.018.82257
GCCGGTC1900.018.5253058
TCTTGCG1850.018.1677862
TAGAGCG450.00884953417.7768065
TCGCGTA904.3629007E-717.7768069
GTCGCGT1006.8064764E-817.599048
TAGACTG1201.6552804E-917.3323885
CCGGTCC1850.017.2963529
CAAGACG2050.017.1697944
GTTTAGG854.932499E-616.9575351
AAGACGG2000.016.7990825
TTAGGAC1051.18676326E-716.762213
GTATAGA600.00242488816.015451
Produced by FastQC (version 0.11.5)