FastQCFastQC Report
Thu 2 Feb 2017
SRR4063044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686767
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA33830.49259792622534276No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC30720.4473132809235156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28460.4144054679389079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20790.30272275749999633No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG15020.2187059075348699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13930.20283444021043529No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC9530.13876613174482758No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8900.1295927148508883No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8160.11881759024530883No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7950.11575978461399572No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG7650.111391490854977No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT7270.10585831876021999No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA7000.10192685437710315No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG200.00375247232.0000043
CGTCGTA1001.4551915E-1020.810
CGTTATT659.424752E-619.6923082
CGGCGTT507.191906E-419.230
ACCGGGC450.0088467517.7777798
TATTAGG550.001363887817.4545462
TCTAGCG1201.6552804E-917.33333428
GTTATTC652.0988529E-417.230773
CGCGGTT1404.0017767E-1117.14285710
TTATACT854.978043E-616.9411774
ATACCGT1252.8467184E-916.646
GGCGTTA600.002440563216.031
CCGTCGT1203.1033778E-816.09
TTTATAC1203.1033778E-816.03
ACCGTCG1203.1033778E-816.08
TACCGTC1203.1033778E-816.07
CTAGCGG1304.789399E-916.029
GTCGTAG1255.125912E-815.3611
TCGAGAA1255.125912E-815.3624
TTAGGTT859.423387E-515.0588244
Produced by FastQC (version 0.11.5)