##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063044_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686767
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15398381110333	32.0	32.0	32.0	32.0	32.0
2	31.289778046994105	32.0	32.0	32.0	32.0	32.0
3	31.37607514630144	32.0	32.0	32.0	32.0	32.0
4	31.482483870075296	32.0	32.0	32.0	32.0	32.0
5	31.402175701511574	32.0	32.0	32.0	32.0	32.0
6	34.93729751138304	36.0	36.0	36.0	36.0	36.0
7	34.968626914222725	36.0	36.0	36.0	36.0	36.0
8	34.90460665698847	36.0	36.0	36.0	36.0	36.0
9	35.02359315459246	36.0	36.0	36.0	36.0	36.0
10	34.86566331812682	36.0	36.0	36.0	32.0	36.0
11	35.034139671824654	36.0	36.0	36.0	36.0	36.0
12	34.943126271355496	36.0	36.0	36.0	36.0	36.0
13	34.97842208492837	36.0	36.0	36.0	36.0	36.0
14	34.92683544783019	36.0	36.0	36.0	32.0	36.0
15	34.90225214665236	36.0	36.0	36.0	32.0	36.0
16	34.9067252794616	36.0	36.0	36.0	32.0	36.0
17	34.871674090339226	36.0	36.0	36.0	32.0	36.0
18	34.85976029716046	36.0	36.0	36.0	32.0	36.0
19	34.85935695803671	36.0	36.0	36.0	32.0	36.0
20	34.84504933987801	36.0	36.0	36.0	32.0	36.0
21	34.821437256012594	36.0	36.0	36.0	32.0	36.0
22	34.80614677175811	36.0	36.0	36.0	32.0	36.0
23	34.72351467091459	36.0	36.0	36.0	32.0	36.0
24	34.70575027629458	36.0	36.0	36.0	32.0	36.0
25	34.686145373904104	36.0	36.0	36.0	32.0	36.0
26	34.60429374154553	36.0	36.0	36.0	32.0	36.0
27	34.57980217453663	36.0	36.0	36.0	32.0	36.0
28	34.53217466768205	36.0	36.0	36.0	32.0	36.0
29	34.491038445353375	36.0	36.0	36.0	32.0	36.0
30	34.470203140220775	36.0	36.0	36.0	32.0	36.0
31	34.45093605254766	36.0	36.0	36.0	32.0	36.0
32	34.41786370049813	36.0	36.0	36.0	32.0	36.0
33	34.37725021732261	36.0	36.0	36.0	32.0	36.0
34	34.3528096137409	36.0	36.0	36.0	32.0	36.0
35	34.28335811126627	36.0	36.0	36.0	32.0	36.0
36	34.26019013726635	36.0	36.0	36.0	32.0	36.0
37	34.22675958512858	36.0	36.0	36.0	32.0	36.0
38	33.78735582810473	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	12.0
22	58.0
23	160.0
24	449.0
25	1060.0
26	2381.0
27	4707.0
28	8233.0
29	13402.0
30	19907.0
31	29076.0
32	41153.0
33	64854.0
34	155674.0
35	345638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.498729439861364	17.47009268900037	11.980253529535972	26.05092434160229
2	15.312616942864175	20.490501145221014	36.80418540786031	27.392696504054502
3	19.12380763781603	24.421528698961946	27.79589001801193	28.658773645210093
4	12.115370054341811	15.981909360420756	35.52137269862718	36.38134788661025
5	13.772065343850242	37.34512578501879	33.39240237227473	15.490406498856235
6	34.73832426183009	35.033038909905265	16.576242854189054	13.652393974075594
7	30.158598416345594	30.43438376390212	20.359773197857788	19.047244621894503
8	28.764927128094087	32.948672958108666	19.141525410154742	19.1448745036425
9	27.388305943134394	14.480692894857167	17.76067285822041	40.370328303788035
10	15.551083645428655	27.264559756303548	31.789747741555953	25.394608856711848
11	37.85108105589339	21.387257985601455	20.842507164325156	19.919153794180005
12	24.20161802307634	24.0375150706793	28.91750295589169	22.843363950352668
13	30.475198939957625	19.525165085582405	24.772374829818062	25.22726114464191
14	23.864571244687063	19.248158400156093	25.12657131166757	31.760699043489275
15	25.741481463145433	26.434292853325804	23.31620476813825	24.508020915390517
16	25.82986660686958	25.711922675376076	24.16350814759591	24.29470257015844
17	23.94917053382006	25.705807064113444	25.723134629357553	24.621887772708938
18	24.959120050905184	24.616354600614184	26.90184589533277	23.522679453147866
19	24.95839200194535	26.034593974375586	25.652805099837355	23.35420892384171
20	25.131522044594455	23.849282216530497	26.961837129623294	24.057358609251757
21	26.234516218746677	24.781039275329185	24.59975508432991	24.384689421594224
22	24.905826867045157	24.79749318182149	25.51724238351581	24.779437567617546
23	23.891030980074667	24.62956707107536	25.70460886126821	25.77479308758176
24	23.909419060612986	25.766235127779876	25.627323386243077	24.69702242536406
25	24.964835184036485	24.409624238823703	25.382007845467015	25.243532731672797
26	23.935483213375132	25.17156473738546	26.096623745753654	24.796328303485755
27	25.41065601579575	24.983145666579787	25.536608485847456	24.06958983177701
28	24.300672571629097	24.544568973174307	26.098225453465297	25.056533001731303
29	23.87534636929264	25.39245479179984	26.589221671978997	24.14297716692852
30	24.72119365083063	25.196027182435966	26.3483830760651	23.734396090668305
31	24.20646303622626	25.307418673290943	25.504283112030716	24.98183517845208
32	24.18607766535084	25.429002849583625	25.57490386113485	24.810015623930678
33	23.731047065453055	24.84379709566709	25.92422175206438	25.50093408681547
34	24.135259847954256	25.38925137637656	26.404588455764472	24.070900319904712
35	25.20228840349056	24.931162970847463	26.125891313939082	23.7406573117229
36	24.137880824209667	25.847339781905653	25.72823097207641	24.28654842180827
37	25.15336351338955	25.61538338330176	25.68789705970147	23.54335604360722
38	24.266780824909794	25.35681731477679	25.57858135085313	24.797820509460283
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	40.5
2	57.0
3	57.0
4	256.5
5	456.0
6	456.0
7	636.0
8	816.0
9	859.0
10	902.0
11	902.0
12	1123.0
13	1344.0
14	1722.0
15	2100.0
16	2100.0
17	3063.5
18	4027.0
19	4027.0
20	4601.5
21	5176.0
22	5276.5
23	5377.0
24	5377.0
25	6015.0
26	6653.0
27	6653.0
28	8660.0
29	10667.0
30	13077.0
31	15487.0
32	15487.0
33	18720.0
34	21953.0
35	21953.0
36	24612.5
37	27272.0
38	31459.5
39	35647.0
40	35647.0
41	39046.5
42	42446.0
43	48272.0
44	54098.0
45	54098.0
46	63213.0
47	72328.0
48	72328.0
49	71441.0
50	70554.0
51	67208.5
52	63863.0
53	63863.0
54	60480.5
55	57098.0
56	57098.0
57	54471.5
58	51845.0
59	46664.5
60	41484.0
61	41484.0
62	37944.5
63	34405.0
64	28549.5
65	22694.0
66	22694.0
67	18932.5
68	15171.0
69	15171.0
70	12233.0
71	9295.0
72	7363.0
73	5431.0
74	5431.0
75	4208.0
76	2985.0
77	2985.0
78	2698.0
79	2411.0
80	1814.5
81	1218.0
82	1218.0
83	1097.5
84	977.0
85	977.0
86	627.5
87	278.0
88	219.5
89	161.0
90	161.0
91	101.0
92	41.0
93	29.0
94	17.0
95	17.0
96	11.5
97	6.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009027807101971994
2	0.0
3	0.0
4	4.368293759018707E-4
5	0.0
6	1.4560979196729022E-4
7	1.4560979196729022E-4
8	0.002038537087542063
9	0.0036402447991822555
10	0.0016017077116401925
11	0.004513903550985997
12	4.368293759018707E-4
13	2.9121958393458044E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	4.368293759018707E-4
24	0.0
25	1.4560979196729022E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.4560979196729022E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	686767.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.82785707636877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86618430943282	46.58566945946936
2	14.17124268881961	16.956701643624715
3	4.135937573819961	7.423328461298522
4	1.6001428344876854	3.829324672140195
5	0.7222929174572053	2.1606618716451553
6	0.35920931289405883	1.2894434059395832
7	0.23872148771900778	0.9997536533817646
8	0.1408619956702637	0.6741977075562092
9	0.09694113417124782	0.5219802288016654
>10	0.5344780430832199	6.1079797555035755
>50	0.06772746905433394	2.843850873462008
>100	0.060636671147293854	6.841988567188799
>500	0.004156546005923942	1.6769622844969931
>1k	0.0014670162373849203	2.088157415491502
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3383	0.49259792622534276	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3072	0.4473132809235156	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2846	0.4144054679389079	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2079	0.30272275749999633	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1502	0.2187059075348699	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1393	0.20283444021043529	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	953	0.13876613174482758	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	890	0.1295927148508883	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	816	0.11881759024530883	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	795	0.11575978461399572	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	765	0.111391490854977	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	727	0.10585831876021999	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	700	0.10192685437710315	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.4560979196729022E-4	7.280489598364511E-4
13	0.0	0.0	0.0	1.4560979196729022E-4	0.0010192685437710315
14	0.0	0.0	0.0	1.4560979196729022E-4	0.0010192685437710315
15	0.0	0.0	0.0	1.4560979196729022E-4	0.001310488127705612
16	0.0	0.0	0.0	1.4560979196729022E-4	0.001310488127705612
17	0.0	0.0	0.0	1.4560979196729022E-4	0.001310488127705612
18	0.0	0.0	0.0	1.4560979196729022E-4	0.001310488127705612
19	0.0	0.0	1.4560979196729022E-4	2.9121958393458044E-4	0.001310488127705612
20	0.0	0.0	1.4560979196729022E-4	2.9121958393458044E-4	0.0014560979196729022
21	0.0	0.0	1.4560979196729022E-4	5.824391678691609E-4	0.0016017077116401925
22	0.0	0.0	1.4560979196729022E-4	8.736587518037413E-4	0.0016017077116401925
23	0.0	0.0	1.4560979196729022E-4	0.0017473175036074825	0.0016017077116401925
24	0.0	0.0	1.4560979196729022E-4	0.003349025215247675	0.0016017077116401925
25	0.0	0.0	1.4560979196729022E-4	0.004805123134920577	0.0016017077116401925
26	0.0	0.0	1.4560979196729022E-4	0.00655244063852806	0.0016017077116401925
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	0.003752472	32.000004	3
CGTCGTA	100	1.4551915E-10	20.8	10
CGTTATT	65	9.424752E-6	19.692308	2
CGGCGTT	50	7.191906E-4	19.2	30
ACCGGGC	45	0.00884675	17.777779	8
TATTAGG	55	0.0013638878	17.454546	2
TCTAGCG	120	1.6552804E-9	17.333334	28
GTTATTC	65	2.0988529E-4	17.23077	3
CGCGGTT	140	4.0017767E-11	17.142857	10
TTATACT	85	4.978043E-6	16.941177	4
ATACCGT	125	2.8467184E-9	16.64	6
GGCGTTA	60	0.0024405632	16.0	31
CCGTCGT	120	3.1033778E-8	16.0	9
TTTATAC	120	3.1033778E-8	16.0	3
ACCGTCG	120	3.1033778E-8	16.0	8
TACCGTC	120	3.1033778E-8	16.0	7
CTAGCGG	130	4.789399E-9	16.0	29
GTCGTAG	125	5.125912E-8	15.36	11
TCGAGAA	125	5.125912E-8	15.36	24
TTAGGTT	85	9.423387E-5	15.058824	4
>>END_MODULE