FastQCFastQC Report
Thu 2 Feb 2017
SRR4063043_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063043_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244651
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34410.2764630406435218No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23020.18495144422010668No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21800.17514949973928434No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20870.1676775256678378No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20240.16261586581298693No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19730.15851833164477433No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18950.15225151468162562No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18770.1508053261516682No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18480.14847535574229243No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18390.1477522614773137No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18140.14574366629681734No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16980.13642378465931415No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16940.1361024094304347No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16920.135941721815995No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15680.12597908972073296No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14820.11906952229982541No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14730.11834642803484671No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14700.11810539661318716No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14690.11802505280596728No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14500.11649852046879004No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT14210.11416855005941424No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14080.11312408056555613No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC13350.10725898263850671No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13190.10597348172298902No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT12870.10340247989195366No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT12770.10259904181975509No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG12630.10147422851867713No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT12530.10067079044647857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGG953.7807695E-818.5303422
TAGGACG802.802568E-617.9959554
TATAACG450.008839603517.7816412
TCTATAC1101.0657459E-817.4513243
TACCGTG652.1028976E-417.2282817
GTACCGT652.1035432E-417.2275896
TTAGGAC753.2391654E-517.0635173
ACCGTCG4700.016.6784428
TACCGTC4900.016.3241737
ATACCGT5050.016.1554346
AATCGTA703.6973757E-415.99961926
TCTAGCG4500.015.99961828
ATATATC805.6303412E-515.9970463
CTAGCGG4600.015.651829
CGCTTCG4350.015.44790732
TATACTT1052.190296E-615.2346715
CGTCTTA951.4329053E-515.15753332
CCGTCGT5350.014.9529159
TCAGATA5600.014.8603712
CATCGTT4200.014.85678828
Produced by FastQC (version 0.11.5)