##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063043_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1244651
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230189024875248	32.0	32.0	32.0	32.0	32.0
2	30.812996574943497	32.0	32.0	32.0	32.0	32.0
3	30.857590601702807	32.0	32.0	32.0	32.0	32.0
4	30.937475645783437	32.0	32.0	32.0	32.0	32.0
5	30.836793607203948	32.0	32.0	32.0	32.0	32.0
6	34.49499096533888	36.0	36.0	36.0	32.0	36.0
7	34.40997114853883	36.0	36.0	36.0	32.0	36.0
8	34.39796778374018	36.0	36.0	36.0	32.0	36.0
9	34.55064351372393	36.0	36.0	36.0	32.0	36.0
10	34.24546238262774	36.0	36.0	36.0	32.0	36.0
11	34.54182015681504	36.0	36.0	36.0	32.0	36.0
12	34.3257973520288	36.0	36.0	36.0	32.0	36.0
13	34.43916728464445	36.0	36.0	36.0	32.0	36.0
14	34.32187416392225	36.0	36.0	36.0	32.0	36.0
15	34.25304523115315	36.0	36.0	36.0	32.0	36.0
16	34.283590339781995	36.0	36.0	36.0	32.0	36.0
17	34.190851893422334	36.0	36.0	36.0	32.0	36.0
18	34.223937473235466	36.0	36.0	36.0	32.0	36.0
19	34.212245038970764	36.0	36.0	36.0	32.0	36.0
20	34.200171775059836	36.0	36.0	36.0	32.0	36.0
21	34.17074826597978	36.0	36.0	36.0	32.0	36.0
22	34.15002840153585	36.0	36.0	36.0	32.0	36.0
23	34.09047355443413	36.0	36.0	36.0	32.0	36.0
24	34.06747513961745	36.0	36.0	36.0	32.0	36.0
25	34.05430116554761	36.0	36.0	36.0	32.0	36.0
26	33.994837910386124	36.0	36.0	36.0	32.0	36.0
27	34.01644798421405	36.0	36.0	36.0	32.0	36.0
28	33.99852970832788	36.0	36.0	36.0	32.0	36.0
29	33.96176036495371	36.0	36.0	36.0	32.0	36.0
30	33.93025273751437	36.0	36.0	36.0	32.0	36.0
31	33.95818747584664	36.0	36.0	36.0	32.0	36.0
32	33.90228184446885	36.0	36.0	36.0	32.0	36.0
33	33.86564908556696	36.0	36.0	36.0	32.0	36.0
34	33.867913977492485	36.0	36.0	36.0	32.0	36.0
35	33.82502082913202	36.0	36.0	36.0	32.0	36.0
36	33.768879790399076	36.0	36.0	36.0	32.0	36.0
37	33.78328382815745	36.0	36.0	36.0	32.0	36.0
38	33.16046425865564	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	7.0
9	3.0
10	9.0
11	4.0
12	4.0
13	3.0
14	165.0
15	361.0
16	434.0
17	515.0
18	670.0
19	852.0
20	1209.0
21	1653.0
22	2453.0
23	3748.0
24	5536.0
25	8339.0
26	12245.0
27	17528.0
28	24809.0
29	33852.0
30	45403.0
31	61643.0
32	84669.0
33	128430.0
34	280379.0
35	529725.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.613368994711124	17.161220262633982	11.265429243670441	25.95998149898445
2	17.255785275772784	20.018858869577173	36.57840940789098	26.146946446759063
3	18.76848137720009	24.37822218793793	28.330424525269667	28.522871909592318
4	12.692529571417325	15.35930015249708	35.91306220241778	36.03510807366782
5	15.11332503113325	36.007713011690036	32.983408990479255	15.895552966697466
6	33.94582243333055	35.488989658120204	16.93016820820023	13.635019700349012
7	30.263664272153402	30.33878573190396	21.188670559056312	18.208879436886324
8	28.506935294155454	31.487778070587026	19.843441679896646	20.161844955360873
9	28.057410609264625	13.960975311895412	18.551188769836934	39.43042530900303
10	16.62488771907836	25.518982907589788	30.498230018816596	27.357899354515258
11	37.79242555837745	21.080752318153223	22.100651356664976	19.026170766804352
12	25.71959078634551	23.510984380109445	27.747420721482914	23.02200411206213
13	29.477635026713262	18.88658455154747	25.15481959609904	26.48096082564023
14	24.410493998275694	19.768689150132698	24.428010242985668	31.392806608605937
15	25.63968425019869	26.401575695333424	22.233552956719006	25.72518709774888
16	26.451861732045572	25.21847429572372	23.094878428977776	25.23478554325293
17	24.51923965494381	25.308754575369548	24.71547328707405	25.456532482612594
18	25.856764650348108	23.738967294581773	25.81715337117697	24.587114683893155
19	26.096839405548554	24.360746149692748	25.11465752191911	24.427756922839585
20	26.52811490379555	23.22003488701893	24.37366873722972	25.878181471955802
21	28.263192655596924	23.48741838704027	23.63027621859016	24.619112738772646
22	26.807239080234424	23.44288586171377	24.300847355489168	25.44902770256264
23	24.62314020767162	23.259366154009324	25.236609535410476	26.880884102908574
24	25.260143382193416	24.630897721666265	24.7404190899843	25.368539806156015
25	25.3906290805622	23.725578902453822	24.854817363578142	26.028974653405832
26	25.138815898377924	24.94097813177506	25.230341981302644	24.689863988544374
27	26.065677829915796	24.079272996078934	24.136883717940478	25.718165456064796
28	24.837155338581105	23.916451267926572	25.50146996081444	25.744923432677886
29	24.80181528043158	24.392750448758022	25.31268430415589	25.492749966654504
30	24.614902540534462	24.683758898298276	25.745368064148096	24.95597049701917
31	25.58432521987019	24.45689290839291	23.904907448027394	26.053874423709505
32	25.14498797190391	24.33178583079032	24.027347246393578	26.49587895091219
33	24.744930344972772	23.814723573433866	24.844322741655297	26.596023339938068
34	25.883410752553388	23.93165895688549	24.975352002333423	25.209578288227696
35	26.77892148590989	23.630402815611216	25.029530168984888	24.56114552949401
36	24.93832874515268	24.81932532048964	24.39023802292001	25.852107911437667
37	26.282968611270697	24.55327406181326	24.352077650244464	24.81167967667158
38	24.98196198921094	24.232560979725115	25.040856697043374	25.744620334020574
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	629.0
1	472.0
2	315.0
3	315.0
4	601.0
5	887.0
6	887.0
7	1099.0
8	1311.0
9	1205.5
10	1100.0
11	1100.0
12	1448.5
13	1797.0
14	2265.5
15	2734.0
16	2734.0
17	4029.5
18	5325.0
19	5325.0
20	6322.0
21	7319.0
22	7504.0
23	7689.0
24	7689.0
25	8675.0
26	9661.0
27	9661.0
28	12429.0
29	15197.0
30	19074.5
31	22952.0
32	22952.0
33	30856.5
34	38761.0
35	38761.0
36	43320.0
37	47879.0
38	55244.5
39	62610.0
40	62610.0
41	67444.5
42	72279.0
43	84146.5
44	96014.0
45	96014.0
46	97752.5
47	99491.0
48	99491.0
49	107694.0
50	115897.0
51	117806.5
52	119716.0
53	119716.0
54	113801.0
55	107886.0
56	107886.0
57	106326.5
58	104767.0
59	93493.5
60	82220.0
61	82220.0
62	80479.0
63	78738.0
64	64682.0
65	50626.0
66	50626.0
67	42473.0
68	34320.0
69	34320.0
70	27823.0
71	21326.0
72	16540.0
73	11754.0
74	11754.0
75	8853.5
76	5953.0
77	5953.0
78	5963.0
79	5973.0
80	4687.0
81	3401.0
82	3401.0
83	3743.5
84	4086.0
85	4086.0
86	2657.0
87	1228.0
88	1111.5
89	995.0
90	995.0
91	725.5
92	456.0
93	403.0
94	350.0
95	350.0
96	327.5
97	305.0
98	305.0
99	504.5
100	704.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11858745945650627
2	0.05415172606618241
3	0.012453290119077557
4	0.0029727208671346426
5	8.034380721985521E-5
6	2.4103142165956558E-4
7	0.0
8	2.4103142165956558E-4
9	2.4103142165956558E-4
10	4.01719036099276E-4
11	0.0014461885299573937
12	0.001606876144397104
13	0.011087445396340018
14	0.0073916302642266785
15	0.01992526419052409
16	0.008918162601403927
17	0.017354262359488722
18	0.004499253204311892
19	0.0060257855414891405
20	0.004981316047631023
21	0.004579597011531747
22	0.00586509792704943
23	0.007230942649786968
24	0.011649852046879005
25	0.014783260528453357
26	0.015827730022311474
27	0.007311286457006823
28	0.004820628433191312
29	0.008436099758084797
30	0.0024906580238155114
31	0.004258221782652326
32	0.005222347469290589
33	0.0071505988425671135
34	0.010284007324141467
35	0.012935352962396687
36	0.011971227275758426
37	0.007713005493106099
38	0.004579597011531747
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1244651.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.177897679549844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.91866458786824	41.41247136643734
2	11.775152810990525	12.052551314438736
3	3.22348012975371	4.949128087577923
4	1.3014516883846494	2.664222453721079
5	0.7200097517631276	1.8424292702005713
6	0.4191938432445126	1.287207577047895
7	0.294666230633605	1.055627874068969
8	0.20934780657623112	0.8571184499517237
9	0.15002482703414685	0.6910159722611157
>10	0.7763154958688335	7.184584479644866
>50	0.08501013448123093	3.093969316095017
>100	0.1032345956564145	11.82708656903671
>500	0.017153306403918763	6.126567254278462
>1k	0.0062947913408876194	4.95602001523964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3441	0.2764630406435218	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2302	0.18495144422010668	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2180	0.17514949973928434	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2087	0.1676775256678378	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2024	0.16261586581298693	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1973	0.15851833164477433	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1895	0.15225151468162562	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1877	0.1508053261516682	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1848	0.14847535574229243	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1839	0.1477522614773137	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1814	0.14574366629681734	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1698	0.13642378465931415	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1694	0.1361024094304347	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1692	0.135941721815995	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1568	0.12597908972073296	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1482	0.11906952229982541	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1473	0.11834642803484671	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1470	0.11810539661318716	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1469	0.11802505280596728	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1450	0.11649852046879004	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1421	0.11416855005941424	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1408	0.11312408056555613	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1335	0.10725898263850671	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1319	0.10597348172298902	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1287	0.10340247989195366	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1277	0.10259904181975509	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1263	0.10147422851867713	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1253	0.10067079044647857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	8.034380721985521E-5	0.0	8.034380721985521E-5	0.0
5	0.0	8.034380721985521E-5	0.0	1.6068761443971042E-4	0.0
6	0.0	8.034380721985521E-5	0.0	1.6068761443971042E-4	0.0
7	0.0	8.034380721985521E-5	0.0	1.6068761443971042E-4	0.0
8	0.0	8.034380721985521E-5	0.0	1.6068761443971042E-4	0.0
9	0.0	8.034380721985521E-5	0.0	1.6068761443971042E-4	0.0
10	0.0	8.034380721985521E-5	0.0	2.410314216595656E-4	0.0
11	0.0	8.034380721985521E-5	0.0	2.410314216595656E-4	0.0
12	0.0	8.034380721985521E-5	0.0	2.410314216595656E-4	0.0
13	0.0	1.6068761443971042E-4	0.0	2.410314216595656E-4	0.0
14	0.0	1.6068761443971042E-4	0.0	2.410314216595656E-4	0.0
15	0.0	1.6068761443971042E-4	0.0	2.410314216595656E-4	0.0
16	0.0	1.6068761443971042E-4	0.0	4.01719036099276E-4	0.0
17	0.0	2.410314216595656E-4	0.0	4.01719036099276E-4	0.0
18	0.0	2.410314216595656E-4	0.0	4.01719036099276E-4	0.0
19	0.0	2.410314216595656E-4	0.0	4.01719036099276E-4	0.0
20	0.0	2.410314216595656E-4	0.0	4.820628433191312E-4	0.0
21	0.0	2.410314216595656E-4	0.0	6.427504577588417E-4	0.0
22	0.0	2.410314216595656E-4	0.0	9.641256866382624E-4	0.0
23	0.0	2.410314216595656E-4	0.0	0.001526532337177249	8.034380721985521E-5
24	0.0	2.410314216595656E-4	0.0	0.0024906580238155114	8.034380721985521E-5
25	0.0	2.410314216595656E-4	0.0	0.003053064674354498	8.034380721985521E-5
26	0.0	2.410314216595656E-4	0.0	0.003535127517673629	8.034380721985521E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGG	95	3.7807695E-8	18.530342	2
TAGGACG	80	2.802568E-6	17.995955	4
TATAACG	45	0.0088396035	17.781641	2
TCTATAC	110	1.0657459E-8	17.451324	3
TACCGTG	65	2.1028976E-4	17.228281	7
GTACCGT	65	2.1035432E-4	17.227589	6
TTAGGAC	75	3.2391654E-5	17.063517	3
ACCGTCG	470	0.0	16.678442	8
TACCGTC	490	0.0	16.324173	7
ATACCGT	505	0.0	16.155434	6
AATCGTA	70	3.6973757E-4	15.999619	26
TCTAGCG	450	0.0	15.999618	28
ATATATC	80	5.6303412E-5	15.997046	3
CTAGCGG	460	0.0	15.6518	29
CGCTTCG	435	0.0	15.447907	32
TATACTT	105	2.190296E-6	15.234671	5
CGTCTTA	95	1.4329053E-5	15.157533	32
CCGTCGT	535	0.0	14.952915	9
TCAGATA	560	0.0	14.860371	2
CATCGTT	420	0.0	14.856788	28
>>END_MODULE