FastQCFastQC Report
Thu 2 Feb 2017
SRR4063043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244651
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43340.34821006049085246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28380.22801572488994906No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23830.19145929260491495No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21450.1723374664865894No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20630.16574927429456127No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19760.1587593630664339No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19600.15747386215091622No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19580.15731317453647647No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19540.15699179930759707No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19430.15610801742817865No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19260.15474217270544113No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA18880.15168910803108662No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18550.1490377623928314No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17230.13843237983981052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17110.13746825415317226No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16090.12927318581674704No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15730.12638080875683222No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15310.12300636885359832No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15030.12075674225144237No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14540.11681989569766946No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14170.11384717483053483No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14040.11280270533667672No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT14000.11248133010779728No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA13990.11240098630057743No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT13980.11232064249335758No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13900.11167789203559873No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC13580.10910689020456336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.10147422851867713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGC400.004484466319.999432
GCGTCAT450.0088522117.77724317
TCTGCGG652.0997734E-417.23163610
CTAGCGG4500.017.06615329
GTATTAC854.980311E-616.9427071
TCTAGCG4350.016.91903128
TAGCGGC4650.016.17155630
ACCGTCG5100.016.0014468
AGGGGCG4200.015.61857832
ATACCGT5250.015.5442636
TACCGTC5250.015.5442637
TCAGATA5300.015.3957622
GTTATAT1255.131733E-815.3613891
CGTCGTA5350.015.25310210
CGGTCCA5250.015.23886110
ACCGGTC756.2405865E-414.9346848
GCTTAAC756.2405865E-414.9346841
TTGCGTC756.2405865E-414.9346849
GTATCAA31250.014.8493421
GTGTTAG1308.301504E-814.7705661
Produced by FastQC (version 0.11.5)