##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063043_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1244651
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.210192254696295	32.0	32.0	32.0	32.0	32.0
2	31.276957958495995	32.0	32.0	32.0	32.0	32.0
3	31.352702082752515	32.0	32.0	32.0	32.0	32.0
4	31.459290194600737	32.0	32.0	32.0	32.0	32.0
5	31.37791155914389	32.0	32.0	32.0	32.0	32.0
6	34.90446960633945	36.0	36.0	36.0	36.0	36.0
7	34.92728644415181	36.0	36.0	36.0	36.0	36.0
8	34.86769303202263	36.0	36.0	36.0	32.0	36.0
9	34.97704738115343	36.0	36.0	36.0	36.0	36.0
10	34.81616533470025	36.0	36.0	36.0	32.0	36.0
11	34.99749809384318	36.0	36.0	36.0	36.0	36.0
12	34.88563380417482	36.0	36.0	36.0	32.0	36.0
13	34.95031137242488	36.0	36.0	36.0	36.0	36.0
14	34.89365372301151	36.0	36.0	36.0	32.0	36.0
15	34.85844706668777	36.0	36.0	36.0	32.0	36.0
16	34.865382344126985	36.0	36.0	36.0	32.0	36.0
17	34.81953013334662	36.0	36.0	36.0	32.0	36.0
18	34.8283534902555	36.0	36.0	36.0	32.0	36.0
19	34.822888504488404	36.0	36.0	36.0	32.0	36.0
20	34.81193362637398	36.0	36.0	36.0	32.0	36.0
21	34.79582308615026	36.0	36.0	36.0	32.0	36.0
22	34.77769029229881	36.0	36.0	36.0	32.0	36.0
23	34.72406401473184	36.0	36.0	36.0	32.0	36.0
24	34.685280452110675	36.0	36.0	36.0	32.0	36.0
25	34.6718011715734	36.0	36.0	36.0	32.0	36.0
26	34.61112793867518	36.0	36.0	36.0	32.0	36.0
27	34.59341453949742	36.0	36.0	36.0	32.0	36.0
28	34.56339568280586	36.0	36.0	36.0	32.0	36.0
29	34.51528420416647	36.0	36.0	36.0	32.0	36.0
30	34.49862330886329	36.0	36.0	36.0	32.0	36.0
31	34.49168642454793	36.0	36.0	36.0	32.0	36.0
32	34.465195464431396	36.0	36.0	36.0	32.0	36.0
33	34.42357978260573	36.0	36.0	36.0	32.0	36.0
34	34.41914480444719	36.0	36.0	36.0	32.0	36.0
35	34.37086942444107	36.0	36.0	36.0	32.0	36.0
36	34.339277436004146	36.0	36.0	36.0	32.0	36.0
37	34.34062078446087	36.0	36.0	36.0	32.0	36.0
38	33.89329538963131	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	6.0
21	20.0
22	94.0
23	310.0
24	848.0
25	2115.0
26	4397.0
27	8552.0
28	14778.0
29	23727.0
30	35910.0
31	51436.0
32	74192.0
33	118881.0
34	287794.0
35	621589.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00343737595958	18.500924829296277	11.927646369703524	26.567991425040617
2	16.085151580643892	21.11933385342558	35.99097256982078	26.804541996109755
3	17.65040963290111	25.186819437737967	28.731427524663538	28.43134340469738
4	12.166733084374055	16.187146888116157	35.96044825524968	35.68567177226011
5	14.54850761258185	36.598160125336435	32.55734543847668	16.29598682360503
6	33.91850547303334	35.95811827922433	16.521859995757836	13.601516251984497
7	29.918579389514143	30.576436068671626	21.131597045911775	18.373387495902456
8	28.31952191107019	31.750844834817315	19.633511218667195	20.2961220354453
9	27.91822337921116	13.726066800041782	18.462168872176825	39.893540948570234
10	16.345514950166113	25.896507811527076	30.555544396549994	27.202432841756817
11	38.018399485778566	20.87795275590551	22.084444801542663	19.01920295677326
12	25.481542543020264	23.69010947691151	27.68658719025329	23.141760789814935
13	29.529875113285044	18.84629228504766	25.2063233942448	26.4175092074225
14	24.43624759069008	19.670333290215492	24.451512914061855	31.441906205032577
15	25.630638628820446	26.425479913646477	22.082977477220524	25.860903980312557
16	26.448618930125793	25.21694836544541	22.989898373118248	25.34453433131054
17	24.550737515978373	25.375547040897406	24.622645223440145	25.451070219684073
18	25.793575869862316	23.700057285134548	25.85238753674725	24.653979308255888
19	26.028340474558732	24.34328980573671	25.17283961528171	24.455530104422845
20	26.45729606130554	23.052727230364177	24.512895582777823	25.977081125552466
21	28.141887277547905	23.52765837785723	23.757923914353434	24.572530430241432
22	26.5790382838549	23.477684489615555	24.556622343630742	25.386654882898807
23	24.493632748162135	23.034427349053953	25.414293174788092	27.05764672799582
24	25.154681914850023	24.49738922798439	24.856204671028266	25.491724186137322
25	25.329691077813038	23.570963724741894	25.053790222150806	26.04555497529426
26	25.242578039948548	24.788956904385245	25.34654292649104	24.621922129175168
27	26.15054340534013	23.855763583526628	24.328506545208256	25.66518646592499
28	24.867211772617384	23.8022546079182	25.670087438165396	25.66044618129901
29	24.866408334545188	24.078155241911187	25.51301529505058	25.542421128493046
30	24.66964635066376	24.290021861549945	25.964065428782845	25.076266359003448
31	25.57568346468207	24.163400021371455	24.161311082383737	26.099605431562743
32	25.287891947220547	24.158177673902163	24.10876623246195	26.44516414641534
33	24.73070764415085	23.503214957445902	25.13580112015336	26.630276278249887
34	25.731952169724686	23.736131654576262	25.29785457931581	25.234061596383246
35	26.775136162667284	23.43186965663467	25.183203966413075	24.609790214284967
36	25.044289523729944	24.595247985178176	24.509922861910688	25.850539629181192
37	26.308499330334367	24.408207601970354	24.474330555312292	24.80896251238299
38	25.024725846403285	23.90127658480423	25.244868231927235	25.829129336865254
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	269.0
1	214.0
2	159.0
3	159.0
4	546.5
5	934.0
6	934.0
7	1131.5
8	1329.0
9	1243.5
10	1158.0
11	1158.0
12	1481.5
13	1805.0
14	2359.0
15	2913.0
16	2913.0
17	4460.5
18	6008.0
19	6008.0
20	6978.5
21	7949.0
22	8028.0
23	8107.0
24	8107.0
25	8873.5
26	9640.0
27	9640.0
28	12420.0
29	15200.0
30	18788.0
31	22376.0
32	22376.0
33	30724.0
34	39072.0
35	39072.0
36	43349.5
37	47627.0
38	55114.5
39	62602.0
40	62602.0
41	67665.0
42	72728.0
43	84303.5
44	95879.0
45	95879.0
46	97510.5
47	99142.0
48	99142.0
49	107933.0
50	116724.0
51	117976.5
52	119229.0
53	119229.0
54	113794.0
55	108359.0
56	108359.0
57	106826.5
58	105294.0
59	93929.5
60	82565.0
61	82565.0
62	80688.0
63	78811.0
64	64700.5
65	50590.0
66	50590.0
67	42653.0
68	34716.0
69	34716.0
70	28115.5
71	21515.0
72	16724.5
73	11934.0
74	11934.0
75	8736.0
76	5538.0
77	5538.0
78	5810.5
79	6083.0
80	4690.5
81	3298.0
82	3298.0
83	3349.0
84	3400.0
85	3400.0
86	2148.5
87	897.0
88	752.0
89	607.0
90	607.0
91	361.5
92	116.0
93	75.5
94	35.0
95	35.0
96	27.5
97	20.0
98	20.0
99	21.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007793349300325956
2	0.0
3	0.0
4	2.4103142165956558E-4
5	8.034380721985521E-5
6	2.4103142165956558E-4
7	2.4103142165956558E-4
8	0.002329970409375801
9	0.0032940960960140637
10	0.0012855009155176833
11	0.004097534168212616
12	4.01719036099276E-4
13	2.4103142165956558E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	8.034380721985521E-5
22	8.034380721985521E-5
23	8.034380721985521E-5
24	0.0
25	8.034380721985521E-5
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.6068761443971042E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1244651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.10621905623816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.46466421397585	39.02209208121908
2	12.624366888096631	12.398698517063856
3	3.5296779449447797	5.199874150872928
4	1.4516186786198026	2.8513401927372404
5	0.7422064617174483	1.8223476547026232
6	0.451806837141242	1.3311915309458369
7	0.3002484728054062	1.0320847093818268
8	0.2068909659578789	0.8127706476067451
9	0.17227078113729857	0.76136100439657
>10	0.8332760685601348	7.398658681724961
>50	0.08591252673539619	3.005876806244457
>100	0.11089861930363677	12.164925054565549
>500	0.018592793292504274	6.414987986376174
>1k	0.0075687477119928915	5.783790982162172
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4334	0.34821006049085246	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2838	0.22801572488994906	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2383	0.19145929260491495	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2145	0.1723374664865894	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2063	0.16574927429456127	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1976	0.1587593630664339	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1960	0.15747386215091622	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1958	0.15731317453647647	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1954	0.15699179930759707	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1943	0.15610801742817865	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1926	0.15474217270544113	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1888	0.15168910803108662	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1855	0.1490377623928314	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1723	0.13843237983981052	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1711	0.13746825415317226	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1609	0.12927318581674704	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1573	0.12638080875683222	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1531	0.12300636885359832	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1503	0.12075674225144237	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1454	0.11681989569766946	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1417	0.11384717483053483	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1404	0.11280270533667672	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1400	0.11248133010779728	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1399	0.11240098630057743	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1398	0.11232064249335758	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1390	0.11167789203559873	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1358	0.10910689020456336	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1263	0.10147422851867713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.034380721985521E-5	0.0
4	0.0	0.0	0.0	8.034380721985521E-5	0.0
5	0.0	0.0	0.0	1.6068761443971042E-4	0.0
6	0.0	0.0	0.0	1.6068761443971042E-4	8.034380721985521E-5
7	0.0	0.0	0.0	2.410314216595656E-4	8.034380721985521E-5
8	0.0	0.0	0.0	2.410314216595656E-4	8.034380721985521E-5
9	0.0	0.0	0.0	4.01719036099276E-4	8.034380721985521E-5
10	0.0	0.0	0.0	4.820628433191312E-4	8.034380721985521E-5
11	0.0	0.0	0.0	4.820628433191312E-4	8.034380721985521E-5
12	0.0	0.0	0.0	4.820628433191312E-4	2.410314216595656E-4
13	0.0	0.0	0.0	4.820628433191312E-4	3.2137522887942083E-4
14	0.0	0.0	0.0	4.820628433191312E-4	3.2137522887942083E-4
15	0.0	0.0	0.0	5.624066505389864E-4	3.2137522887942083E-4
16	0.0	0.0	0.0	7.230942649786968E-4	3.2137522887942083E-4
17	0.0	0.0	0.0	7.230942649786968E-4	3.2137522887942083E-4
18	0.0	0.0	0.0	8.03438072198552E-4	3.2137522887942083E-4
19	0.0	0.0	0.0	8.03438072198552E-4	3.2137522887942083E-4
20	0.0	0.0	0.0	8.837818794184073E-4	3.2137522887942083E-4
21	0.0	0.0	0.0	9.641256866382624E-4	4.01719036099276E-4
22	0.0	0.0	0.0	0.0012855009155176833	4.01719036099276E-4
23	0.0	0.0	0.0	0.0018479075660566696	4.820628433191312E-4
24	0.0	0.0	0.0	0.002812033252694932	4.820628433191312E-4
25	0.0	0.0	0.0	0.0033744399032339186	4.820628433191312E-4
26	0.0	0.0	0.0	0.0036958151321133392	5.624066505389864E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACGC	40	0.0044844663	19.9994	32
GCGTCAT	45	0.00885221	17.777243	17
TCTGCGG	65	2.0997734E-4	17.231636	10
CTAGCGG	450	0.0	17.066153	29
GTATTAC	85	4.980311E-6	16.942707	1
TCTAGCG	435	0.0	16.919031	28
TAGCGGC	465	0.0	16.171556	30
ACCGTCG	510	0.0	16.001446	8
AGGGGCG	420	0.0	15.618578	32
ATACCGT	525	0.0	15.544263	6
TACCGTC	525	0.0	15.544263	7
TCAGATA	530	0.0	15.395762	2
GTTATAT	125	5.131733E-8	15.361389	1
CGTCGTA	535	0.0	15.253102	10
CGGTCCA	525	0.0	15.238861	10
ACCGGTC	75	6.2405865E-4	14.934684	8
GCTTAAC	75	6.2405865E-4	14.934684	1
TTGCGTC	75	6.2405865E-4	14.934684	9
GTATCAA	3125	0.0	14.849342	1
GTGTTAG	130	8.301504E-8	14.770566	1
>>END_MODULE